CORE--DNA SEQUENCING FACILITY

核心——DNA测序设备

• 批准号: 7310498
• 负责人: Robert C Young
• 金额: $ 10.44万
• 依托单位: FOX CHASE CANCER CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7310498
• 关键词: DNA; biomedical facility; genetic polymorphism; high throughput technology; neoplasm /cancer; nucleic acid sequence

The Automated DNA Sequencing Facility is a merged Facility which provides investigators with state-of-the-art DNA sequencing and analysis of DNA polymorphisms. The Facility is used by 52 peer-reviewed funded investigators across 10 Research Programs from all three Divisions. Ninety-nine and one-half percent (99.5%) of the users have peer-reviewed funding. Richard Hardy, Ph.D. (Program Leader, Immunobiology) assumed direction of the Facility in 1993. Automated sequencing using an ABI Model 373 was set up in 1994. Model 377 sequencers were subsequently added, one in 1995, and another in 1997. A third 377 replaced the original 373 in 2002. Recently, two of the 377s have been replaced by a modern ABI 3100 capillary instrument, providing more reliable high-throughput sequencing. The Sequencing Facility provides Fox Chase Cancer Center (FCCC) investigators with computer-readable sequences of their DNA samples in a timely and cost effective manner, generating data more rapidly and of greater read-length compared to manual sequencing. Automated sequence analysis is used by investigators to verify DNA constructs, to identify mutations, and to determine the structure of newly cloned genes. Sequencing large genes (5-20 Kb) is much more tractable using automated sequence analysis, generating results in a few weeks, as compared to months using manual sequencing. The volume of usage of the Facility has continued to increase steadily over the past five years from 17,000 sequences in 1998 to over 28,000 in 2003 (64% increase). In addition to sequencing, analysis of DNA polymorphisms, important in genetic mapping studies, is also available in the Facility using "GeneScan" software on the 377. Equitable access to the Facility is assured by a Faculty Oversight Committee. The Facility was rated "Outstanding" at the last CCSG review.

自动DNA测序设施是一个合并的设施，可为研究人员提供 DNA多态性的最新DNA测序和分析。该设施由52使用 在所有三个部门的10个研究计划中，通过同行评审的研究人员进行了资助。 99％和一半（99.5％）的用户进行了同行评审的资金。理查德·哈迪（Richard Hardy）博士(Program Leader, Immunobiology) assumed direction of the Facility in 1993. Automated sequencing using an ABI Model 373 was set up in 1994. Model 377 sequencers were subsequently added, one in 1995, and another in 1997. A third 377 replaced the original 373 in 2002. Recently, two of the 377s have been replaced by a modern ABI 3100 capillary instrument, providing more reliable high-throughput测序。该测序设施以及时且具有成本效益的方式为FOX Chase Cance Center（FCCC）研究者提供了其DNA样品的可读序列，与手动测序相比，更快地生成数据的读取长度。研究人员使用自动序列分析来验证DNA构建体，鉴定突变并确定新克隆基因的结构。与使用手动测序的几个月相比，使用自动序列分析，使用自动序列分析进行测序大基因（5-20​​ kb）可以在几周内产生结果。在过去的五年中，该设施的使用量从1998年的17,000个序列增加到2003年的28,000多年（增加64％）。除了测序外，在基因映射研究中对DNA多态性的分析也可以在377上使用“ Genescan”软件​​在设施中提供。 该设施由教师监督委员会保证。该设施被评为“出色” 最后的CCSG评论。