Core--Pathology and Tissue Resource

核心--病理与组织资源

• 批准号: 7614974
• 负责人: D CRAIG ALLRED
• 金额: $ 27.63万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7614974
• 关键词: Apoptosis; Biological Assay; Bromodeoxyuridine; Clinical; Confocal Microscopy; Development; Ensure; Histology; Human; Image Analysis; Immunohistochemistry; In Situ Hybridization; Invasive; Paraffin; Pathology; Premalignant; Preparation; Purpose; Services; Slide; Specimen; Staining method; Stains; TdT-Mediated dUTP Nick End Labeling Assay; Tissue Microarray; Tumor Tissue; Work; assay development; breast lesion; cost; follow-up; laser capture microdissection; malignant breast neoplasm; prognostic; programs; tissue processing; tissue resource

DESCRIPTION (provided by applicant): The purpose of the Pathology and Tissue Resource (PTR) Core is to provide comprehensive histology support, pathology support, and tumor and tissue resources to all five projects of this program. Pathology services will include tissue processing, slide preparation and staining, preparation of tissue arrays, laser capture microdissection, IHC assay development and analysis, in situ hybridization assays, BrdU staining, apoptosis (TUNEL) assays, and confocal microscopy and image analysis. As well as managing and providing access to existing tumor and tissue resources, the PTR Core will also assemble and maintain two new tissue resources that will be used by the projects. One is a retrospective bank of archival paraffin blocks of human invasive breast cancers with clinical follow-up to support prognostic/predictive studies, which will include approximately 2000 cases with extended follow-up, assembled into tissue arrays. The other is a retrospective bank of archival paraffin blocks of human premalignant breast lesions to support studies on the early development and progression to invasive breast cancer ? this bank will include approximately 1200 specimens, also assembled into tissue arrays. Overall, many thousands of blocks and slides will be prepared, stained, and evaluated over the duration of the Program. Performing the work in the PTR Core will ensure that it is done in the most expert, timely, and cost-efficient manner possible.

描述（由申请人提供）：病理和组织资源（PTR）的目的 核心是为该计划的所有五个项目提供全面的组织学支持，病理支持以及肿瘤和组织资源。病理服务将包括组织加工，幻灯片制备和染色，组织阵列的制备，激光捕获显微解剖，IHC测定开发和分析，原位杂交测定，BRDU染色，凋亡（TUNEL）测定（TUNEL）测定以及共焦显微镜和图像分析。除管理和提供获得现有肿瘤和组织资源的访问外，PTR核心还将组装和维护两个项目将使用的新组织资源。一个是人类浸润性乳腺癌的档案石蜡回顾性库，具有临床随访，以支持预后/预测性研究，其中包括大约2000例具有扩展随访的病例，并组装成组织阵列。另一个是人类前乳腺病变的档案石蜡块回顾性银行，以支持对早期发育和发展为侵入性乳腺癌的研究？该银行将包括大约1200个标本，还将组装到组织阵列中。总体而言，在该计划的过程中，将准备，染色和评估成千上万个块和幻灯片。在PTR核心中执行工作将确保以最专业，及时和成本效益的方式完成工作。