Intravital Microscopy

活体显微镜检查

基本信息

批准号：
7534128
负责人：
ULRICH H VON ANDRIAN
金额：
$ 24.59万
依托单位：
SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-06-01 至 2013-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7534128
关键词：
Adhesions Animals Bladder Blood Cells Blood Platelets Bone Marrow Cell Adhesion Cell Communication Cells Color Dimensions Ear Elements Environment Equipment Fluorescence Hematopoietic System Homing Human Resources Image Imaging Device Immune response Immunity In Vitro Individual Inflammation Inguinal lymph node group Investigation Knee joint Lamina Propria Leukocytes Liver Mesentery Microscopy Modeling Mus Pathway interactions Physiological Property Range Research Personnel Resolution Role Services Site Skin Small Intestines Structure of aggregated lymphoid follicle of small intestine Thrombosis Time Tissues base cell motility cremaster muscle design experience glycosylation glycosyltransferase in vivo intravital microscopy migration multi-photon programs research study

Adhesions Animals Bladder Blood Cells Blood Platelets Bone Marrow Cell Adhesion Cell Communication Cells Color Dimensions Ear Elements Environment Equipment Fluorescence Hematopoietic System Homing Human Resources Image Imaging Device Immune response Immunity In Vitro Individual Inflammation Inguinal lymph node group Investigation Knee joint Lamina Propria Leukocytes Liver Mesentery Microscopy Modeling Mus Pathway interactions Physiological Property Range Research Personnel Resolution Role Services Site Skin Small Intestines Structure of aggregated lymphoid follicle of small intestine Thrombosis Time Tissues base cell motility cremaster muscle design experience glycosylation glycosyltransferase in vivo intravital microscopy migration multi-photon programs research study

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项目摘要

Cellular adhesion and migration mechanisms have been recognized as crucial elements regulating the fate and function of all leukocytes. As such, the direct visalization of cell adhesion properties and the fine structural feature that influence these properties is central to our understanding of how white blood cells Darticipate in inflammation, hemostasisT thrombosis and immunity. The Intravital Microscopy Core has been :ormulated based on a stated need of PPG investigators and is designed to draw on the considerable experience of its key personnel to provide expertise and specialized equipment in the design and execution of in vivo imaging studies on the pathophysiological role of glycosyltransferases. The Specific Aims of the Intravital Microscopy Core are as follows: 1. To execute and analyze intravital microscopy-based adhesion and migration studies in murine tissues. Currently available imaging models include: cremaster muscle; ear skin; bone marrow; liver; popliteal and inguinal lymph node; bladder; knee joint; and small intestine, including mesentery, Peyer's patches and lamina propria. 2. To provide access to and expertise and to conduct specific experiments utilizing multi-photon microscopy-based recording of intra- and extravascular blood cell adhesion, migration and cell-cell interactions in as many as six dimensions (i.e. space, time, color, fluorescence intensity). 4. To provide assistance in the planning, execution and analysis of blood cell homing in vivo employing defined models of inflammation. Services to be provided by the Imaging Core will allow participating investigators to investigate how defined glycosylation pathways influence blood cells interactions with their environment at a resolution ranging from single cells to intact animals.

细胞粘附和迁移机制已被认为是调节命运的关键要素和所有白细胞的功能。因此，细胞粘附特性的直接粘附和细细胞粘附影响这些特性的结构特征对于我们对白细胞的理解至关重要炎症，止血性血栓形成和免疫力中的darticipate。插入显微镜核心一直是：根据PPG调查人员的既定需求进行操作，旨在利用可观的其关键人员的经验，可以在设计和执行中提供专业知识和专业设备关于糖基转移酶的病理生理作用的体内成像研究。插入显微镜核心的特定目的如下： 1。在鼠组织中执行和分析基于插入显微镜的粘附和迁移研究。当前可用的成像模型包括：Cremaster肌肉；耳朵皮；骨髓；肝; popliteal 和腹股沟淋巴结；膀胱;膝盖关节；和小肠，包括肠系膜，佩耶的补丁和lamina propria。 2。提供使用和专业知识的访问并使用多光子进行特定的实验基于显微镜的记录血管内和血管外血细胞粘附，迁移和细胞细胞的记录多达六个维度（即空间，时间，颜色，荧光强度）的相互作用。 4。在体内的血细胞归巢计划，执行和分析方面提供帮助定义的炎症模型。成像核心提供的服务将允许参与的调查人员调查定义的定义糖基化途径会影响血细胞与环境相互作用的分辨率从单细胞到完整动物。