Physiology and Development of the Vertebrate Retina

脊椎动物视网膜的生理学和发育

• 批准号: 7681214
• 负责人: Z JIMMY ZHOU
• 金额: $ 41.38万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-08-01 至 2012-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7681214
• 关键词: Address; Appearance; Calcium; Cells; Complex; Development; Developmental Process; Disease; Eye; Frequencies; Generations; Glutamates; Health; Image; Inner Plexiform Layer; Interneurons; Laboratories; Lead; Measurement; Measures; Mediating; Nature; Neurons; Oryctolagus cuniculus; Pacemakers; Physiology; Process; Property; Research Personnel; Retina; Retinal; Role; Series; Slice; Specific qualifier value; Staging; Synapses; Synaptic Transmission; Techniques; Testing; Time; Vision; Work; base; cholinergic; flash photolysis; ganglion cell; network models; neural circuit; novel; patch clamp; programs; synaptic function; synaptogenesis; vision development

DESCRIPTION (provided by applicant): This application proposes to continue our ongoing studies of the mechanisms of spontaneous rhythmic activity and the establishment of functional synaptic circuits in the developing mammalian retina. It will test two hypotheses: (1) the spontaneous waves that appear during two different developmental stages (conventional and ribbon synaptogenesis in the inner retina) are produced by different networks of intrinsic oscillators; (2) the complex, asymmetric synaptic circuitry involving starburst cells is established by a process of coordinated reorganization and refinement of synaptic connectivity and synaptic function. To test these hypotheses, we propose to integrate several cutting-edge electrophysiological techniques, which will enable the direct measurement of synaptic function and synaptic connectivity in an anatomically well-defined and functionally well-specified network at various developmental stages. The proposed studies will pursue three specific aims: (1) to determine the cellular mechanisms underlying spontaneous retinal waves, (2) to determine the mechanism and function of synaptic interaction during synaptogenesis.and retinal waves, (3) to understand the development of starburst synaptic function and circuitry. Results from these studies will allow the integration of functional and anatomical information about the developing retina in a way previously unattainable in other experimental settings and may lead to better understanding of visual system development in health and diseases.

描述（由申请人提供）：本申请建议继续我们对自发节奏活动机理的持续研究以及在发育中的哺乳动物视网膜中建立功能突触电路。它将检验两个假设：（1）在两个不同的发育阶段出现的自发波（内部视网膜中的常规和色带突触发生）是由不同的内在振荡器网络产生的； （2）通过协调的重组和突触连通性和突触功能的完善过程，建立了涉及Starburst细胞的复合物突触电路。为了检验这些假设，我们建议在各种发育阶段在解剖上定义明确的且功能良好的网络中直接测量突触功能和突触连通性。拟议的研究将追求三个具体目的：（1）确定自发性视网膜波的基础机制，（2）确定突触发生过程中突触相互作用的机制和功能。这些研究的结果将允许以其他实验环境无法实现的方式整合有关发展视网膜的功能和解剖信息，并可能会更好地了解健康和疾病中的视觉系统发展。