TRANSGENIC MOUSE

转基因小鼠

基本信息

批准号：
7714291
负责人：
Kathleen Joyce Millen
金额：
$ 16.06万
依托单位：
UNIVERSITY OF CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-07-22 至 2013-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7714291
关键词：
Brain Breeding Cancer Center Cells Chicago Colon Development ES Cell Line Embryo Equipment Facility Construction Funding Category Faculty Female Gene Expression Regulation Gene Targeting Generations Genetic Engineering Genome Genus Cola Hematopoietic System Human Resources Individual Injection of therapeutic agent Laboratory mice Maintenance Malignant Neoplasms Microinjections Modeling Molecular Genetics Mus Numbers Patient currently pregnant Pregnancy Preparation Procedures Production Range Research Research Design Research Personnel Services Signal Transduction Skin Staging Technology Time Transgenic Animals Transgenic Mice Universities University of Chicago Cancer Research Center animal resource blastocyst cancer therapy cost embryonic stem cell experience malignant breast neoplasm member mouse model programs research facility tool

项目摘要

The UCCRC Transgenic Mouse/Embryonic Stem Cell Facility provides comprehensive genetic engineering services to alter the genome of the laboratory mouse. The UCCRC has many investigators who use mouse models as their primary tool of analysis, as well as a multitude of additional investigators who require the occasional mouse model in their studies of the causes and treatment of cancer. The generation and maintenance of transgenic animals through the microinjection of singlecelled mouse embryos, and the generation of genetically modified mice through the use of ES cells require specialized technical personnel. Furthermore, such efforts necessitate the acquisition of an array of devoted equipment. Thus, the availability of a shared facility greatly reduces research costs for the individual investigators of the UCCRC. The existence of this facility also greatly increases the accessibility of genetic engineering technology to investigators with limited related experience. The UCCRC Transgenic Mouse/Embryonic Stem Cell Facility was established in 1991, and has been tremendously productive and successful; the range of services provided has expanded considerably since its founding. Nine services are provided by the Transgenic Mouse/Embryonic Stem Cell Facility: (1) transgenic mouse production from founder through F1 Stage; (2) ES cell technology mouse production; (3) ES cell gene targeting and culturing; (4) mouse embryonic feeder (MEF) cell production; (5) Timed pregnancies of various strains and lines of mice; (6) embryo rederivation; (7) ES cell line development; (8) various breeding services and GEM model line maintenance; and (9) DMA preparation from ES cell lines for the construction of a gene targeting construct. In addition to the technical services, the Facility also offers assistance with the design of studies that require mouse molecular genetics and an annual course in Mouse Handling and Breeding. This course covers information regarding analysis and handling of the mice once the Facility has generated them, as well as general breeding and handling procedures for mice. In providing these comprehensive services, the UCCRC Transgenic Mouse/Embryonic Stem Cell Facility has generated mouse models that have led to advances in our understanding of cancer, including cancers of the breast, skin, colon, brain and hematopoietic system.

UCCRC转基因小鼠/胚胎干细胞设施提供全面的遗传工程服务改变实验室鼠标的基因组。 UCCRC有许多调查员谁使用鼠标模型作为他们的主要分析工具，以及许多其他在研究原因和治疗的研究中，需要偶尔的小鼠模型的研究者癌症。通过微注射单粒子的生成和维持转基因动物小鼠胚胎，以及通过使用ES细胞的转基因小鼠的产生需要专门的技术人员。此外，这样的努力需要获得一系列虔诚的设备。因此，共享设施的可用性大大降低了研究成本 UCCRC的个体调查员。该设施的存在也大大增加了基因工程技术可访问有限的经验的研究人员。 UCCRC转基因小鼠/胚胎干细胞设施于1991年建立，已经是富有成效和成功；提供的服务范围已大大扩展自成立以来。九种服务由转基因小鼠/胚胎干细胞设施提供：（1）从创始人到F1阶段的转基因小鼠产生；（2）ES细胞技术鼠标生产; （3）ES细胞基因靶向和培养；（4）小鼠胚胎喂食器（MEF）细胞产生；（5）各种菌株和小鼠的定时怀孕；（6）胚胎重新培养；（7）ES细胞系发展; （8）各种育种服务和宝石模型线维护；（9）DMA 从ES细胞系制备基因靶向构建体的构建。除了技术服务，该设施还为需要鼠标的研究设计提供了帮助分子遗传学和小鼠处理和繁殖的年度课程。本课程涵盖一旦设施产生了有关小鼠的分析和处理的信息，作为小鼠的一般育种和处理程序。在提供这些综合服务时 UCCRC转基因小鼠/胚胎干细胞设施产生了导致的小鼠模型我们对癌症的理解的进步，包括乳房，皮肤，结肠，大脑和造血系统。