PRODUCTION OF PEDIGREED SPF RHESUS MACAQUES: HIV VACCINE DVMT

纯种 SPF 恒河猴的生产：HIV 疫苗 DVMT

• 批准号: 7724643
• 负责人: NICHOLAS WILLIAM LERCHE
• 金额: $ 30.13万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7724643
• 关键词: Acquired Immunodeficiency Syndrome; Age; Alleles; Animals; Biological Models; Breeding; Cercopithecine Herpesvirus 1; Class; Computer Retrieval of Information on Scientific Projects Database; Cytomegalovirus; Data; Funding; Genotype; Goals; Grant; HIV vaccine; Immune System Diseases; Immune response; Institution; Lymphocyte Subset; MHC Class II Genes; Macaca; Macaca mulatta; Microsatellite Repeats; Mothers; Nurseries; Parents; Phenotype; Production; Reference Values; Research; Research Personnel; Resources; Rhadinovirus; SIV; Simian Foamy Virus; Simian Retroviruses; Source; Type D Retrovirus; United States National Institutes of Health; Viral; Virus; age related; cohort; comparative; genetic pedigree; genetic profiling; germ free condition; neonate; programs; social group; vaccine development

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The goal of this proposal is to expand the production of genetically characterized rhesus macaques of Indian-ancestry that are specific pathogen-free (SPF) for an expanded list of 7 persistent viruses, including simian immunodeficiency virus (SIV), simian type D retrovirus (SRV), simian T-lymphotropic virus (STLV), Cercopithecine herpesvirus 1 (B virus), simian foamy virus (SFV), rhesus cytomegalovirus (RhCMV) and rhesus rhadinovirus (RRV). This proposed expansion will be geared to the production of SPF animals for use in AIDS-related research, and to establishing a long-term SPF breeding colony of Indian-origin rhesus macaques with known pedigrees and well-defined MHC genotypes. To meet these objectives, we will derive SPF offspring from conventional, non-SPF Indian rhesus breeding stock through nursery-rearing of neonates. In addition, we will expand the existing Indian-origin colony that is SPF for 5 persistent viruses (SIV, SRV, STLV, SFV, B virus) through natural breeding and mother-rearing in social groups. An expanded program of MHC typing that includes additional microsatellite loci, class I alleles, and class II genes will be used to characterize offspring and their parents. This expanded MHC typing will allow the identification and definition of immune response genotypes, and will allow for selected breeding to produce animals with specific genetic profiles. We also propose to collect longitudinal data on age-related changes in the major lymphocyte subsets in cohorts of nursery-reared and mother-reared SPF rhesus macaques. Age-specific reference ranges, developed from normal, healthy SPF-rhesus macaques, are essential to more fully understand and utilize the macaque model system, and to provide comparative data allowing for more accurate interpretation of lymphocyte subset phenotype alterations observed in AIDS and other infectious and immunologic diseases. The rhesus monkeys produced as part of this grant will provide valuable resources for AIDS-related research. These animals will be used for research related to vaccine development and anti-viral therapy.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 描述（由申请人提供）： The goal of this proposal is to expand the production of genetically characterized rhesus macaques of Indian-ancestry that are specific pathogen-free (SPF) for an expanded list of 7 persistent viruses, including simian immunodeficiency virus (SIV), simian type D retrovirus (SRV), simian T-lymphotropic virus (STLV), Cercopithecine herpesvirus 1 (B病毒），猿猴泡沫病毒（SFV），恒河猴（RHCMV）和恒河猴rhadinovirus（RRV）。该拟议的扩张将用于生产SPF动物，用于与艾滋病相关的研究中，并建立了具有已知的谱系和明确定义的MHC基因型的印度原生恒河猕猴的长期SPF繁殖菌落。为了实现这些目标，我们将通过新生儿育苗的传统，非SPF印度恒河猴繁殖库存得出SPF后代。此外，我们还将通过社交群体中的自然育种和养育母亲来扩展现有的印度 - 源性菌落，该菌落是5种持续病毒（SIV，SRV，STLV，SFV，B病毒）的SPF。扩展的MHC键入程序，其中包括其他微卫星基因座，I类等位基因和II类基因，将用于表征后代及其父母。扩展的MHC键入将允许鉴定和定义免疫反应基因型，并允许选定的育种产生具有特定遗传特征的动物。我们还建议收集有关年龄相关的纵向数据，以了解幼儿园和母亲的SPF恒河猕猴的主要淋巴细胞子集的变化。特定年龄的参考范围是从正常的，健康的SPF逆转猕猴开发的，对于更充分的理解和利用猕猴模型系统至关重要，并提供了比较数据，从而可以更准确地解释淋巴细胞亚群表型在AIDS和其他感染和免疫疾病中观察到的淋巴细胞子集表型改变。作为这笔赠款的一部分生产的恒河猴将为与艾滋病相关的研究提供宝贵的资源。这些动物将用于与疫苗发育和抗病毒疗法有关的研究。