IMAGING & HOSTOPATH

影像学

• 批准号: 7802102
• 负责人: FU-SHIN YU
• 金额: $ 8.07万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7802102
• 关键词: Anatomy; Animals; Applications Grants; Area; Award; Beds; Biochemistry; Cancer Center Support Grant; Cells; Cellular biology; Collaborations; Computer Graphics; Computer Workstations; Computer software; Computers; Data; Digital Photography; Electron Microscopy; Electrons; Equipment; Fixatives; Floor; Freezing; Funding; Funding Opportunities; Goals; Histopathology; Housing; Image; Image Analysis; Immunochemistry; Immunofluorescence Immunologic; Immunology; Individual; Lasers; Light; Link; Manuscripts; Methods; Microbiology; Microscope; Microscopic; Microscopy; Microtome - medical device; Molecular Biology; Morphology; Names; Neurosciences; Ophthalmologist; Outcome; Photography; Population; Preparation; Production; Productivity; Publications; Published Comment; Research; Research Personnel; Research Training; Resolution; Scanning; Scanning Electron Microscopy; Scientist; Site; Slide; System; Talents; Technical Expertise; Tissues; Training; Vision; Vision research; Work; base; digital; digital imaging; image processing; interest; meetings; posters; protocol development; square foot; tissue culture; tissue processing; transmission process

The Imaging/Histopathology Module is a centralized facility in the Anatomy/Cell Biology Department that provides light, confocal laser scanning, and electron microscopic expertise with priority of use to NEI funded Core vision scientists. The Module has been expanded and enhanced with Core grant, Departmental, Institutional, and individual R01 support and offers a wide range of capabilities which will not only enhance ongoing NEI-funded work but which will facilitate new funding opportunities and collaborations as evidenced below. The Module contains two large pieces of equipment, the JEOL 1010 transmission EM and the NEI awarded Leica TSC SP2 confocal laser scanning microscope (the latter is newly added since the last competing renewal of the Core grant). The module is housed on two floors of Scott Hall. A 1900 sq. ft. facility is located in rooms 7341, 7345, 7347, 7350, 7354 in Scott Hall and this site provides for confocal laser scanning and electron microscopy, tissue processing and sectioning, immunochemistry, and slit lamp photography. The 8th floor houses the other suite of Module dedicated rooms and includes 1,200 sq. ft of space (rooms 8344, 8350, 8352 and 8354). This area includes the Zeiss Axiophot and Zeiss Axiocam image processing and analysis microscope system as well as the Zeiss Apotome microscope. The area also includes computer workstation areas, 3 MacinTosh and 5 PC computers, flat bed scanner capability, 8 printers and a variety of software (including Metamorph). Superb technical support, equipment oversight and training to investigators is provided by skilled research assistants, Mr. Ronald Barrett (7th floor) and Mrs. Yaoying Wang (8th floor). Collaboration within the Module occurs in a variety of ways. Most basically, the Module provides skilled technical expertise and well-maintained equipment to NEI funded individuals who are not primarily morphologists. For example, an ophthalmologist may need to document by morphological or other photographic methods a cell population that he is interested in isolating and characterizing or growing in the Core tissue culture facility. The type of assistance provided by the Module for this need is at a simple, yet important level to that individual. Collaboration may also consist of assistance in experimental protocol development, determining appropriate fixatives to be utilized, interpretation of morphological data, photographic figure preparation, digital graphics applications, poster and slide production, and lastly, the possibility of collaboration in manuscript submission. It is also the goal of the Module to enhance, at lower priority, collaboration and productivity of those who are seeking NEI funding by providing technical assistance, equipment availability and interaction with vision researchers of diverse fields. These types of collaborations and interactions are currently provided for by the facility and it is the goal of the Module co-directors to continue to encourage and expand them.

成像/组织病理学模块是解剖学/细胞生物学部门的集中设施，该设施 提供光线，共聚焦激光扫描和电子显微镜专业知识，并优先使用。 核心视觉科学家。该模块已通过核心赠款，部门的扩展和增强， 机构和个人R01支持，并提供广泛的功能，不仅可以增强 持续的Nei资助工作，但这将促进新的资金机会和合作 以下。 该模块包含两个大型设备，Jeol 1010变速器EM和NEI授予 Leica TSC SP2共聚焦激光扫描显微镜（后者是自上次竞争以来新添加的 续签核心赠款）。该模块位于斯科特大厅的两层楼。 1900平方英尺的设施位于 Scott Hall的7341、7345、7347、7347、7354的房间，该站点提供了共聚焦激光扫描和 电子显微镜，组织加工和切片，免疫化学和缝隙摄影。第八 地板设有另一间模块专用房间的套件，包括1,200平方英尺的空间（房间8344，8350， 8352和8354）。该区域包括Zeiss Axiophot和Zeiss Axioocam图像处理和分析 显微镜系统以及Zeiss Apotome显微镜。该地区还包括计算机工作站 区域，3台Macintosh和5台电脑，平床扫描仪功能，8个打印机和各种软件 （包括变质）。提供了出色的技术支持，设备监督和对调查人员的培训 由熟练的研究助理罗纳德·巴雷特（Ronald Barrett）先生（7楼）和Yaoying Wang夫人（8楼）。 模块内的协作以多种方式进行。基本上，该模块提供熟练的 技术专业知识和良好的设备为不主要是的NEI资助的个人 形态学家。例如，眼科医生可能需要通过形态学或其他 摄影方法是他有兴趣隔离和表征或生长的细胞种群 核心组织培养设施。该模块为该需求提供的帮助类型很简单，但 对于那个人而言，重要的水平。协作也可能包括实验协议的帮助 开发，确定要使用的适当固定剂，形态数据的解释， 摄影人像制备，数字图形应用程序，海报和幻灯片制作，最后是 提交手稿合作的可能性。它也是该模块增强的目标 通过提供技术援助来寻求NEI资金的人的优先，协作和生产力， 设备可用性和与各种领域的视力研究人员的互动。这些类型的合作 该设施目前为互动提供了互动，这是模块联合导演继续的目标 鼓励和扩展它们。