Imaging Core

成像核心

• 批准号: 7625008
• 负责人: Joshua Trachtenberg
• 金额: $ 23.01万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-19 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7625008
• 关键词: Algorithms; Anatomy; Anterior; Cataloging; Catalogs; Computer software; Development; Genes; Image; Image Analysis; Interneurons; Kinetics; Label; Laboratories; Length; Life; Neurons; Operative Surgical Procedures; Preparation; Protocols documentation; Research Personnel; Resources; Role; Techniques; Testing; Time; Transgenic Mice; Vertebral column; Visual; Work; calcium indicator; cingulate cortex; cost; cost effective; density; hippocampal pyramidal neuron; in vivo; member; multidisciplinary; novel; relating to nervous system; sensor; tool; voltage

The function of the Imaging Core is to test novel imaging approaches and image analysis algorithms, streamline these techniques for in vivo imaging of cortical plasticity, and supply the optimized protocols and software to the investigators in the Center. The approaches optimized by the Imaging Core will be used in Projects 1, 2 and 3 of the Center for multidisciplinary, comprehensive studies of the role of specific genes in anatomical cortical plasticity. The Core is a cost effective and efficient way of providing imaging and analysis techniques to investigators in the Center. Specifically, the Core will: 1. Screen novel transgenic mice for markers for in vivo imaging that label specific aspects of neural anatomy, such as specific inhibitory interneurons and laminar-specific pyramidal neurons. 2. Catalog which labeled lines are bright enough for in vivo imaging and how this brightness changes with development. 3. Catalog which labeled lines express in visual, barrel, and anterior cingulate cortices 4. Screen newly-advanced genetically-encoded markers of neuronal function (e.g. calcium indicators and voltage sensors) to determine which are suitable for in vivo imaging. 5. Develop novel image analysis tools to automate the quantification of axonal and dendritic structural plasticity (e.g. changes in branching, branch length, spine volume, spine and bouton density, spine kinetics). 6. Continually fine-tune the surgical preparation for live imaging of cortical neurons. By assigning these tasks to one facility, the Center gains an economy of scale in which the time devoted to working through these important issues is absorbed once by the Core. Thus, each lab in the Center is not individually burdened with the costs in time, people, and resources necessary to work through and optimize the imaging approaches necessary to accomplish the projects proposed by the Center. Additionally, the Core will insure that the imaging approach used by each laboratory is uniformly agreed upon by each of the members of the Center and is of the highest possible quality.

成像核的功能是测试新型成像方法和图像分析算法， 简化这些技术用于在体内形成皮质可塑性的成像，并提供优化的方案和 向中心的调查人员进行软件。成像芯优化的方法将用于 多学科中心的项目1、2和3，综合研究特定基因在 解剖学皮质可塑性。 核心是一种经济有效，有效的方式，可以向研究人员提供成像和分析技术 中心。具体而言，核心将： 1。用于体内成像标记的新型转基因小鼠，标记神经的特定方面 解剖学，例如特定的抑制性中间神经元和层状特异性锥体神经元。 2。标记线的目录足够明亮，可以体内成像以及这种亮度如何随着 发展。 3。在视觉，枪管和前扣带回皮层中标记为线条的目录 4。神经元功能的筛选遗传编码标记（例如钙指标和 电压传感器）以确定适用于体内成像的哪个。 5。开发新颖的图像分析工具以自动轴突和树突结构的定量 可塑性（例如分支，分支长度，脊柱体积，脊柱和胸部密度的变化，脊柱动力学）。 6。不断微调皮质神经元实时成像的手术准备。 通过将这些任务分配给一个设施，该中心获得了规模经济的经济性，在这种情况下，专门用于 解决这些重要问题的工作被核心吸收一次。因此，中心的每个实验室不是 单独负担时间，人员和资源的负担 完成中心提出的项目所需的成像方法。另外， 核心将确保每个实验室使用的成像方法都均匀地同意 中心成员，质量最高。