Task Specific Project 2

任务特定项目 2

• 批准号: 7728715
• 负责人: Katsuo Kurabayashi
• 金额: $ 5.35万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-29 至 2013-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7728715
• 关键词: Air; Architecture; Articular Range of Motion; Automobile Driving; Aviation; Caliber; Cells; Compatible; Computers; Contrast Media; Depth; Electromagnetics; Electrostatics; Endoscopes; Evaluation; Figs - dietary; Growth; Human; Image; Light; Liquid substance; Mechanics; Micrometastasis; Microscope; Motion; Mucous Membrane; Muscularis Mucosa; Numbers; Penetration; Pliability; Range of motion exercise; Scanning; Specimen; Speed; Staging; Structure; System; Tissues; Topical application; base; density; design; desire; in vivo; neoplastic; novel; optical fiber; prototype; size; tumor progression

The confocal miniprobe (Cell-viZio-GI¿) used in Specific Aim 2 provides horizontal cross-sectional images up to 100 (im tissue penetration depth. This distance is insufficient to assess for the presence of neoplastic invasion and micrometastases below the muscularis mucosa (Fig 3b), which is needed to accurately stage the progression of cancer. We have achieved vertical cross-sectional images with 500 ^m tissue penetration depth ex vivo using the novel dual axes confocal architecture (Fig 8b). This orientation is preferred :for pathological evaluation because it reveals the natural growth and proliferation of tissue micro-structures. Furthermore, we have developed a 5 mm diameter (endoscope compatible) dual axes prototype that achieves high speed scanning in the horizontal (xy-) plane using a MEMS (micro-electro-mechanical sytems) mirror. In order to achieve vertical cross-sections, we need to develop an axial (z-) scanner that performs with sufficient speed for in vivo imaging. MEMS actuators have been developed previously for a number of applications, including computer hard drives,82'83 aviation fluid control systems,84 and optical fiber manipulators.85 These designs are based on electrostatic, thermal, or electromagnetic principles. Howev.er, each of these approaches have limitation in either the amount of force that can be generated or by the size and power required to move the scanhead over the desired range of motion. Pneumatic actuation offers a reliable, robust approach for axial displacement that provides many of the features needed to meet in the in vivo scanning requirements, including 1) high power/force density, 2) large motion displacement, 3) design flexibility, and 4) variety in choice of driving medium. The actuation speed is inherently less than that for MEMS-based electrostatic, thermal, and electromagnetic approaches, but a recent study has shown that pneumatic (air-driven) actuators can achieve axial motions as large as 300 urn at 5 Hz.86 This bandwidth is sufficient for the dual axes confocal microscope to achieve vertical cross-sectional images of 500 jam penetration depth using 785 nm light.

特定目标2中使用的共聚焦微型型（细胞 - 维齐 - gi）提供了水平的横截面图像 至100（IM组织穿透深度。此距离不足以评估肿瘤的存在 肌肉粘膜下方的侵袭和微型转移（图3b），需要准确地分期 癌症的进展。我们已经实现了500 ^M组织穿透的垂直横截面图像 使用新型的双轴共聚焦结构的深度离体（图8B）。这种方向是优选的： 病理评估是因为它揭示了组织微结构的自然生长和增殖。 此外，我们已经开发了一个直径5毫米（内窥镜兼容）双轴原型 使用MEMS（微机械系统）镜像在水平（XY-）平面中进行高速扫描。 为了实现垂直横截面，我们需要开发一个轴向（z-）扫描仪，以足够 体内成像的速度。 MEMS执行器以前已经针对许多应用程序开发了，包括计算机硬计算机 驱动器，82'83航空控制系统，84和光纤操纵器。85这些设计基于 静电，热或电磁原理。 howev.er，这些方法中的每一种都有限制 可以产生的力量，或者通过将扫描头移动所需的大小和功率 所需的运动范围。气动活动为轴向位移提供了一种可靠，可靠的方法 提供满足体内扫描要求所需的许多功能，包括1）高 功率/力密度，2）大型运动位移，3）设计灵活性，4）选择驾驶的多样性 中等的。启动速度本质上比基于MEMS的静电，热和 电磁方法，但最近的一项研究表明，气动（气动）执行器可以实现 轴向运动在5 hz.86以上的轴向运动足以使双轴共聚焦显微镜 为了实现500 JAM穿透深度的垂直横截面图像，使用785 nm的光。