Task Specific Project 2

任务特定项目 2

• 批准号: 7728715
• 负责人: Katsuo Kurabayashi
• 金额: $ 5.35万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-29 至 2013-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7728715
• 关键词: Air; Architecture; Articular Range of Motion; Automobile Driving; Aviation; Caliber; Cells; Compatible; Computers; Contrast Media; Depth; Electromagnetics; Electrostatics; Endoscopes; Evaluation; Figs - dietary; Growth; Human; Image; Light; Liquid substance; Mechanics; Micrometastasis; Microscope; Motion; Mucous Membrane; Muscularis Mucosa; Numbers; Penetration; Pliability; Range of motion exercise; Scanning; Specimen; Speed; Staging; Structure; System; Tissues; Topical application; base; density; design; desire; in vivo; neoplastic; novel; optical fiber; prototype; size; tumor progression

The confocal miniprobe (Cell-viZio-GI¿) used in Specific Aim 2 provides horizontal cross-sectional images up to 100 (im tissue penetration depth. This distance is insufficient to assess for the presence of neoplastic invasion and micrometastases below the muscularis mucosa (Fig 3b), which is needed to accurately stage the progression of cancer. We have achieved vertical cross-sectional images with 500 ^m tissue penetration depth ex vivo using the novel dual axes confocal architecture (Fig 8b). This orientation is preferred :for pathological evaluation because it reveals the natural growth and proliferation of tissue micro-structures. Furthermore, we have developed a 5 mm diameter (endoscope compatible) dual axes prototype that achieves high speed scanning in the horizontal (xy-) plane using a MEMS (micro-electro-mechanical sytems) mirror. In order to achieve vertical cross-sections, we need to develop an axial (z-) scanner that performs with sufficient speed for in vivo imaging. MEMS actuators have been developed previously for a number of applications, including computer hard drives,82'83 aviation fluid control systems,84 and optical fiber manipulators.85 These designs are based on electrostatic, thermal, or electromagnetic principles. Howev.er, each of these approaches have limitation in either the amount of force that can be generated or by the size and power required to move the scanhead over the desired range of motion. Pneumatic actuation offers a reliable, robust approach for axial displacement that provides many of the features needed to meet in the in vivo scanning requirements, including 1) high power/force density, 2) large motion displacement, 3) design flexibility, and 4) variety in choice of driving medium. The actuation speed is inherently less than that for MEMS-based electrostatic, thermal, and electromagnetic approaches, but a recent study has shown that pneumatic (air-driven) actuators can achieve axial motions as large as 300 urn at 5 Hz.86 This bandwidth is sufficient for the dual axes confocal microscope to achieve vertical cross-sectional images of 500 jam penetration depth using 785 nm light.

Specific Aim 2 中使用的共焦微型探针 (Cell-viZio-GI¿) 提供水平横截面图像 至 100（im 组织穿透深度）。此距离不足以评估是否存在肿瘤 粘膜肌层以下的侵袭和微转移（图 3b），这是准确分期所必需的 我们已经获得了具有 500 微米组织穿透力的垂直横截面图像。 使用新型双轴共焦架构进行深度体外分析（图 8b）。 病理学评价，因为它揭示了组织微结构的自然生长和增殖。 此外，我们还开发了直径 5 毫米（内窥镜兼容）双轴原型，可实现 使用 MEMS（微机电系统）镜在水平 (xy) 平面上进行高速扫描。 为了获得垂直横截面，我们需要开发一种轴向 (z-) 扫描仪，其性能具有足够的性能 体内成像速度。 MEMS 执行器之前已开发用于多种应用，包括计算机硬件 驱动器，82'83 航空流体控制系统，84 和光纤操纵器。85 这些设计基于 然而，这些方法中的每一种都具有局限性。 可以产生的力的大小或者移动扫描头所需的尺寸和功率 气动驱动提供了一种可靠、稳健的轴向位移方法。 提供了满足体内扫描要求所需的许多功能，包括 1) 高 功率/力密度，2) 大运动位移，3) 设计灵活性，以及​​ 4) 驱动选择的多样性 驱动速度本质上低于基于 MEMS 的静电、热和驱动速度。 电磁方法，但最近的一项研究表明气动（空气驱动）执行器可以实现 5 Hz 时的轴向运动高达 300 urn。86 此带宽足以用于双轴共焦显微镜 使用 785 nm 光获得 500 堵塞穿透深度的垂直横截面图像。