Molecular mechanisms of Foxc-mediated angiogenesis

Foxc介导的血管生成的分子机制

基本信息

批准号：
10198028
负责人：
Tsutomu Kume
金额：
$ 46.22万
依托单位：
NORTHWESTERN UNIVERSITY AT CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-07-01 至 2023-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10198028
关键词：
Affect Amino Acid Transporter Amino Acids Anterior eyeball segment structure Axenfeld-Rieger syndrome Blood Blood Vessels Blood capillaries Brain Cardiovascular Diseases Cells Cellular Metabolic Process Cerebral small vessel disease Data Defect Development Disease Dose Embryo Endothelial Cells Essential Amino Acids Eyelash FOXC1 gene FOXC2 gene FRAP1 gene Family Filopodia Foxes Gene Expression Genes Genetic Glutamine Goals Growth Factor Heart Human Impairment Knock-in Knock-in Mouse Knock-out Lead Leucine Link Lymphedema Mediating Metabolic Mission Molecular Molecular Genetics Mus Mutant Strains Mice Mutation Non-Essential Amino Acid Pathologic Pathologic Neovascularization Pathway interactions Patients Pattern Physiologic Neovascularization Process Public Health Research Retina Role Series Severities Signal Transduction Symptoms Tamoxifen Testing Transcriptional Regulation Transportation United States National Institutes of Health angiogenesis base blood vessel development density experimental study improved lymphedema-distichiasis syndrome member mutant postnatal retina blood vessel structure targeted treatment transcription factor

项目摘要

Formation of the blood vasculature depends on the precise control of molecular networks that are tightly regulated by proangiogenic growth factors and by molecules involved in endothelial cell (EC) metabolism. However, the transcriptional control of these processes remains incompletely understood. The long-term goal of our lab is to elucidate the fundamental mechanisms that regulate the formation of blood vessels and to understand how the disruption of these mechanisms leads to vascular defects in pathological settings. FOXC1 and FOXC2 are closely related members of the FOX (Forkhead box) transcription factor family and have critical roles in vascular development and disease. Mutations or changes in the copy number of human FOXC1 are associated with autosomal-dominant Axenfeld-Rieger syndrome (ARS), which is characterized by anterior eye segment defects and cerebral small vessel disease, while inactivating mutations of FOXC2 are responsible for autosomal-dominant lymphedema-distichiasis syndrome, which includes symptoms such as late-onset lymphedema and extra eyelashes (distichiasis). We have completed preliminary experiments suggesting (1) that the mutations are associated with declines in filopodia formation and proliferation at the angiogenic front, defects in vascular density and branching in the capillary plexus, and impaired vascular patterning; and (2) that these angiogenic defects are accompanied by significant declines in the activity of mammalian target of rapamycin (mTOR) and in the expression of CD98, which imports essential amino acids such as leucine while exporting the nonessential amino acid glutamine. Thus, our central hypothesis is that the Foxc transcription factors participate in physiological and pathological angiogenesis by regulating pathways involved in amino acid transport, EC metabolism, and mTOR signaling. Guided by strong preliminary data, this hypothesis will be tested by pursuing two specific aims: 1) identify the molecular and genetic networks that link the Foxc transcription factors with angiogenesis and EC metabolism; 2) Define the mechanisms by which Foxc1 and Foxc2 cooperatively participate in physiological and pathological angiogenesis. In summary, the results generated from the experiments described in this proposal will provide crucial information about the formation of blood vessels; thus, because vascular deficiencies are among the leading causes of cardiovascular disease and disorders, our findings are likely to identify new targets and therapeutic strategies for improving vascular formation and function in affected patients.

血管系统的形成取决于紧密结合的分子网络的精确控制受促血管生成生长因子和参与内皮细胞（EC）代谢的分子调节。然而，这些过程的转录控制仍不完全清楚。长期目标我们实验室的目标是阐明调节血管形成的基本机制并了解这些机制的破坏如何导致病理环境中的血管缺陷。福克斯C1 和 FOXC2 是 FOX（叉头盒）转录因子家族密切相关的成员，并且具有在血管发育和疾病中发挥重要作用。人类 FOXC1 拷贝数的突变或变化与常染色体显性遗传的 Axenfeld-Rieger 综合征 (ARS) 有关，该综合征的特征是前房眼节缺陷和脑小血管疾病，而 FOXC2 失活突变是造成这种情况的原因用于常染色体显性遗传性淋巴水肿-连串综合征，包括迟发性等症状淋巴水肿和多余的睫毛（双鞭毛病）。我们已经完成了初步实验建议（1）这些突变与血管生成前沿丝状伪足形成和增殖的下降有关，毛细血管丛的血管密度和分支缺陷以及血管模式受损；和（2）这些血管生成缺陷伴随着哺乳动物靶标活性的显着下降。雷帕霉素 (mTOR) 和 CD98 的表达，CD98 导入亮氨酸等必需氨基酸，同时输出非必需氨基酸谷氨酰胺。因此，我们的中心假设是 Foxc 转录因子通过调节涉及氨基的途径参与生理和病理性血管生成酸转运、EC 代谢和 mTOR 信号传导。在强有力的初步数据的指导下，这一假设将是通过追求两个特定目标进行测试：1）识别连接 Foxc 的分子和遗传网络与血管生成和 EC 代谢相关的转录因子； 2) 定义 Foxc1 和 Foxc2协同参与生理和病理血管生成。综上所述，结果从本提案中描述的实验中生成的结果将提供有关形成的重要信息血管；因此，因为血管缺陷是心血管疾病的主要原因之一和疾病，我们的研究结果可能会确定改善血管的新靶点和治疗策略受影响患者的形成和功能。