ADENYLATE CYCLASE-ASSOCIATED COMPLEXES IN SCHIZOSACCHAROMYCES POMBE

粟酒裂殖酵母中腺苷酸环化酶相关复合物

• 批准号: 7602230
• 负责人: CHARLES S. HOFFMAN
• 金额: $ 0.62万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7602230
• 关键词: Adenylate Cyclase; Affect; Carbon; Collection; Complement; Complex; Computer Retrieval of Information on Scientific Projects Database; Cyclic AMP; Environment; Fission Yeast; Funding; G Protein-Coupled Receptor Genes; Genes; Grant; Heterotrimeric GTP-Binding Proteins; Institution; Mutation; Proteins; Protocols documentation; Research; Research Personnel; Resources; Signal Transduction; Source; United States National Institutes of Health; Yeasts; genetic analysis

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our lab has identified mutations in several genes required for adenylate cyclase activation in fission yeast. These genes encode the subunits of a heterotrimeric G protein and a likely GPCR, along with three genes not previously known to be associated with cAMP signaling. We also have a collection of mutations in the adenylate cyclase gene that appear to affect activation but not catalytic activity. We would like to complement our genetic analyses with a study of the proteins physically associated with adenylate cyclase in fission yeast as a function of the carbon source environment. We would also like to study the effect of mutations within adenylate cyclase on these interactions. We have constructed an integrated TAP-tagged form of adenylate cyclase at its endogenous locus and are able to detect it by Western analysis. We are now using the "Purification of TAP-Tagged Proteins" protocol from the Yeast Resource Center to allow us to isolate protein complexes from this strain.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 我们的实验室已经确定了裂变酵母中腺苷酸环化酶激活所需的几种基因中的突变。这些基因编码异三聚体G蛋白和可能的GPCR的亚基，以及以前已知与CAMP信号传导相关的三个基因。我们在腺苷酸环化酶基因中也有一系列突变，这些突变似乎影响激活，但不影响催化活性。我们想通过对裂变酵母中与腺苷酸环化酶物理相关的蛋白质的研究来补充我们的遗传分析，这是碳源环境的函数。我们还想研究腺苷酸环化酶内突变对这些相互作用的影响。我们已经在其内源性基因座上构建了一种腺苷酸环化酶的综合tap标签形式，并能够通过西方分析检测到它。现在，我们正在使用酵母资源中心的“纯化标记蛋白的纯化”方案，以使我们能够将蛋白质复合物与该菌株分离。