STRUCTURAL STUDIES OF RNA FOLDING INTERMEDIATES

RNA折叠中间体的结构研究

• 批准号: 7601779
• 负责人: Tobin R Sosnick
• 金额: $ 0.79万
• 依托单位: ILLINOIS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7601779
• 关键词: Adopted; Base Sequence; Biological Process; Catalytic RNA; Chemicals; Class; Computer Retrieval of Information on Scientific Projects Database; Data; Event; Funding; Goals; Grant; Institution; Life; Maps; Measurement; Methods; Modeling; Modification; Process; RNA; RNA Folding; Research; Research Personnel; Resources; Roentgen Rays; Role; Shapes; Source; Spectrum Analysis; Structure; Trefoil Motif; United States National Institutes of Health; base; macromolecule; movie; size; structural biology; three dimensional structure; time use

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. RNA molecules including ribozymes form another major class of structurally important biomolecules. Identifying the structure and folding mechanisms is critical for understanding their function. The goal of the proposal is to discern the important folding steps by identifying the structure of the folding intermediates. This information will used to further understand the function of this particular RNA, and folding and function of RNA molecules in general. Structural RNAs adopt 3D structures which are essential for their biological function. Predicting the 3D structure from the nucleotide sequence has proven remarkably difficult and remains a central problem in structural biology. The goal of this project is to understand the folding using time-resolved (sec) small-angle X-ray scattering (SAXS) methods. One of the fundamental structural parameters of a macromolecule is its over-all size and shape. SAXS can provide this information and is extremely well-suited for characterizing partially folded, short-lived states. The proposed measurements will be extremely useful in determining the sequence of events and, in particular the role of compaction in the folding process of the S-domain of the P RNA ribozyme. These data will be compared to those obtained using chemical modification and spectroscopy, and will greatly assist model building to produce a movie of the folding process. In particular, we wish to distinguish between four models based upon preliminary structural mapping data . These models have a Rg differing by 1  10 ¿, which is within the expected experimental accuracy.

该副本是使用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这是调查员的机构。 RNA分子包括核酶，形成了另一类重要的结构重要生物分子。识别结构和折叠机制对于理解其功能至关重要。该建议的目的是通过识别折叠中间体的结构来辨别重要的折叠步骤。该信息将用于进一步了解该特定RNA的功能，以及RNA分子的折叠和功能。 结构RNA采用3D结构，这对于其生物功能至关重要。事实证明，预测核苷酸序列的3D结构非常困难，并且在结构生物学中仍然是一个核心问题。该项目的目的是使用时间分辨（SEC）小角度X射线散射（SAXS）方法来了解折叠。大分子的基本结构参数之一是其大小和形状。 SAXS可以提供​​此信息，非常适合表征部分折叠的短寿命状态。所提出的测量将在确定事件序列，尤其是压实在P RNA核酶S域的折叠过程中的作用非常有用。这些数据将与使用化学修饰和光谱法获得的数据进行比较，并将大大帮助模型构建以制作折叠过程的电影。特别是，我们希望根据初步结构映射数据进行区分四个模型。这些模型的RG分化为1 10？，这在预期的实验精度范围内。