THE MOLECULAR GENETIC BASIS OF SENSORY BEHAVIOR IN DROSOPHILA

果蝇感觉行为的分子遗传基础

• 批准号: 7661644
• 负责人: Paul Garrity
• 金额: $ 28.96万
• 依托单位: BRANDEIS UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7661644
• 关键词: Affect; Agnosia; Animal Behavior; Animals; Behavior; Biological Models; Body Temperature; Control Animal; Crabs; Dementia; Drosophila genus; Drosophila melanogaster; Esthesia; Exhibits; Genetic; Genetic Screening; Goals; Hallucinations; Heating; Homarus; Human; Lobster; Maine; Malignant Neoplasms; Mental disorders; Molecular; Molecular Genetics; Neurons; Pain; Pathway interactions; Perception; Perceptual Disorders; Physical environment; Physiology; Process; Property; Proteins; RNA Interference; Research; Resistance; Sensory; Sensory Process; Stimulus; Stroke; Temperature; Temperature Sense; Testing; Variant; Work; base; extreme temperature; fly; insight; interest; migration; neural circuit; receptor; response; sensor; somatosensory

Temperature is a ubiquitous environmental variable with major effects on physiology and behavior, and animals exhibit strong temperature-dependent orientation behaviors. However, the mechanisms that govern such orientation behaviors remain largely unknown. In this proposal, we propose to characterize the circuitry and the molecular mechanisms that guide this temperature-regulated behavior using molecular genetics in Drosophila. The aims of this proposal are to: Aim 1: Identify the neurons governing thermotactic behavior. We will identify and assess the function of neurons involved in thermotaxis by using genetic approaches to ablate and inhibit the function of specific neurons. Aim 2: Examine the molecular properties of regulators of thermotaxis and test the hypothesis that candidate thermosensory neurons act by triggering repulsion from extreme temperatures. Aim 3: Investigate the molecular basis of differences in themosensory behavior and candidate thermal sensors. Aim 4: Identify additional regulators of themotaxis using a combination of RNAi-based and conventional genetic screens. The goal of this research is to understand the molecular mechanisms and neural circuits that control animal behavior, by focusing on the molecules and neuronal pathways involved in sensing and responding to environmental stimuli. Our long-term experimental goal is to obtain a molecular and cellular explanation of how Drosophila undergoes directed migration guided by temperature differentials. Our results should provide insight into how animals process sensory information in order to discriminate between subtle gradations in sensory input, a topic of relevanceto human perceptual disorders like agnosia resulting from stroke or dementia and hallucination associated with mental illness. Our work will also enhance understanding of the basic mechanisms of temperature sensation. Temperature perception is highly relevant to the perception of pain, and our studies should provide insights into how such somatosensory information is sensed and processed.

温度是无处不在的环境变量，对生理和行为以及 动物表现出强烈的温度依赖性方向行为。但是，管理的机制 这种定向行为在很大程度上是未知的。在此提案中，我们建议表征电路 以及使用分子遗传学指导这种温度调节行为的分子机制 果蝇。该提案的目的是： AIM 1：确定控制热功能行为的神经元。我们将确定并评估 通过使用遗传方法消融并抑制特定功能的神经元， 神经元。 AIM 2：检查热门调节剂的分子特性，并检验候选者的假设 热感觉神经元通过触发极端温度的排斥而起作用。 AIM 3：研究效能行为差异和候选热的分子基础 传感器。 AIM 4：使用基于RNAi和常规的组合确定themotaxis的其他调节剂 遗传筛选。 这项研究的目的是了解控制动物的分子机制和神经回路 行为，专注于传感和响应涉及的分子和神经元途径 环境刺激。我们的长期实验目标是获得分子和细胞的解释 果蝇如何在温度差异引导下进行定向迁移。我们的结果应该 提供有关动物如何处理感官信息以区分微妙的信息的见解 感官输入的等级，这是一个相关的人类感知障碍等主题，例如由agnosia产生 中风或痴呆症以及与精神疾病相关的幻觉。我们的工作也将增强 了解温度感觉的基本机制。温度感知高度相关 为了感知疼痛，我们的研究应提供有关这种体感信息的见解 被感知和处理。