JC VIRUS EXPRESSION IN THE OROPHARYNX AND HIV

JC 病毒在口咽部和 HIV 中的表达

• 批准号: 7607344
• 负责人: Joseph R Berger
• 金额: $ 0.13万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7607344
• 关键词: Acquired Immunodeficiency Syndrome; Blood; Blood specimen; CD4 Lymphocyte Count; Computer Retrieval of Information on Scientific Projects Database; Detection; Frequencies; Funding; Grant; Immunosuppression; Individual; Institution; JC Virus; Leukocytes; Liquid substance; Measures; Oropharyngeal; Patients; Persons; Pharyngeal structure; Polymerase Chain Reaction; Prevalence; Progressive Multifocal Leukoencephalopathy; Research; Research Personnel; Resources; Saliva; Sampling; Source; Therapeutic immunosuppression; United States National Institutes of Health; Virus; Whole Blood; foot; peripheral blood

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This study is an exploratory analysis of the difference in the prevalence of the JC Virus in oropharyngeal secretions and blood of healthy individuals, persons with Acquired Immune Deficiency Syndroms (AIDS), and pes with progressive multifocal leukoencephalopathy (PML). Saliva and throat washings as well as whole blood samples will be taken from each group. Saliva samples and throat washings will be analyzed for JCV using polymerase chain reaction (PCR). Whole blood samples will be collected and analyzed for JCV using PCR and for the presence of JCV in peripheral blood leukocytes (PBLs) using CD4 lymphocyte counts. These measures will be used to determine whether JCV is harbored in the oropharynx, whether a correlation exists between JCV levels in the oropharynx and those in PBLs and to determine whether the level and strain of JCV shed from the oropharynx correlates with the degree of immunosuppression. It is predicted that JCV will be present in oropharyngeal fluids, the frequency of detection of JCV will be increased in patients with AIDS-associated PML compared with healthy controls and HIV-seropositive patients without PML and that higher levels of the JC Virus will correlate with increased immunosuppression.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 这项研究是对健康个体的口咽分泌物和血液中JC病毒患病率差异的探索性分析，患有获得性免疫缺陷综合征（AIDS）的人以及具有渐进性多焦点白细胞性脑病（PML）的PE。唾液和喉咙清洗以及全部血液样本将从每组中采集。使用聚合酶链反应（PCR）分析JCV的唾液样品和喉咙清洗。将使用PCR收集并分析全血样品，并使用CD4淋巴细胞计数在外周血白细胞（PBL）中存在JCV。这些措施将用于确定JCV是否在口咽中含有含有的JCV，在口咽和PBL中的JCV水平之间是否存在相关性，并确定从口咽的JCV水平和应变是否与免疫抑制程度相关。据预测，与健康对照组和没有PML的HIV症状患者相比，与AIDS相关的PML患者的JCV将存在于口咽液中，JCV的检测频率将增加，而没有PML的HIV症状患者，JC病毒的较高水平将与免疫抑制的增加相关。