IN VIVO STUDIES OF THE PI PATHWAY IN BIPOLAR DISORDER

双相情感障碍 PI 通路的体内研究

• 批准号: 7627522
• 负责人: JAIR C SOARES
• 金额: $ 5.98万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7627522
• 关键词: Bipolar Disorder; Blood Platelets; Blood specimen; Brain; Brain scan; Computer Retrieval of Information on Scientific Projects Database; Condition; DSM-IV; Densitometry; Depressed mood; Development; Diagnosis; Diagnostic; Disease; Functional disorder; Funding; Grant; Inositol; Institution; Lasers; Lithium; Magnetic Resonance Spectroscopy; Manic; Measurement; Membrane; Pathway interactions; Patients; Phosphatidylinositols; Protons; Recruitment Activity; Research; Research Personnel; Resources; Scanning; Source; Thin Layer Chromatography; United States National Institutes of Health; clinically relevant; frontal lobe; in vivo; two-dimensional

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. OBJECTIVE: The phosphoinositol (PI) pathway may be abnormal in bipolar disorder, and this could be an important mechanism involved in its pathophysiology and the mechanisms of action of lithium and other treatments for this condition. This study will investigate in vivo the intracellular PI pathway in bipolar disorder patients, and examine the hypothesis of a dysfunction in this pathway in patients suffering from this disorder. RESEARCH PLAN: We will recruit 90 un-medicated bipolar subjects (30 depressed, 30 euthymic, 30 manic), 30 lithium-treated euthymic patients, and 60 matched healthy controls over the course of a 5 year period. Patients will be diagnosed according to the DSM-IV criteria and need to meet diagnostic criteria for bipolar disorder type I. METHODS: Patients will provide blood samples for determination of platelet membrane phosphoinositides, which will be done with two-dimensional thin-layer chromatography followed by scanning laser densitometry. They will also undergo a 1.5T proton magnetic resonance spectroscopy (MRS) brain scan, which will allow the quantitation of may-inositol in a single voxel placed in the frontal cortex. Correlations will be examined between brain myo-inositol and platelet phosphoinositide levels and between these measurements and specific disease states. The impact of lithium treatment will also be examined. CLINICAL RELEVANCE: This study may result in substantial contributions to the understanding of the pathophysiology of bipolar disorder and ultimately contribute to the development of new treatments for this condition.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 目的：磷酸肌醇（PI）途径可能异常在躁郁症中，这可能是其病理生理学涉及的重要机制以及锂和其他治疗方法的作用机理。 这项研究将在双相情感障碍患者的细胞内PI途径中研究，并检查患有这种疾病患者的该途径功能障碍的假设。 研究计划：我们将招募90名未经介绍的双极受试者（30名抑郁症，30个正式，30个躁狂），30例锂治疗的正常患者和60个匹配的健康对照，在5年的过程中。 患者将根据DSM-IV标准进行诊断，并需要满足I型双相情感障碍诊断标准。 方法：患者将提供血液样本以测定血小板膜磷酸肌醇，这将用二维薄层色谱法进行，然后进行扫描激光密度测定法。 他们还将进行1.5T质子磁共振光谱（MRS）脑扫描，这将允许在放置在额叶皮质中的单个体素中定量五月互感。 将在脑肌醇和血小板磷酸辛醇水平以及这些测量和特定疾病状态之间检查相关性。 还将检查锂治疗的影响。 临床相关性：这项研究可能会对对躁郁症的病理生理学的理解产生重大贡献，并最终有助于为这种疾病的新治疗发展。