Adipocyte development and insulin resistance

脂肪细胞发育和胰岛素抵抗

• 批准号: 7593408
• 负责人: Vipul Periwal
• 金额: $ 9.82万
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7593408
• 关键词: Adipocytes; Adipose tissue; Animals; Appearance; Behavior; Biopsy; Cell Size; Characteristics; Collaborations; Condition; Data; Development; Diabetes Mellitus; Goals; Hyperglycemia; Insulin; Insulin Resistance; Laboratories; Lipids; Metabolic; Modeling; National Institute of Diabetes and Digestive and Kidney Diseases; Non-Insulin-Dependent Diabetes Mellitus; Obesity; Periodicity; Play; Protocols documentation; Publications; Rattus; Relative (related person); Role; Role playing therapy; Sampling; Statistically Significant; Techniques; Time; Work; adipocyte differentiation; day; interest; size; Adipocytes; Adipose tissue; Animals; Appearance; Behavior; Biopsy; Cell Size; Characteristics; Collaborations; Condition; Data; Development; Diabetes Mellitus; Goals; Hyperglycemia; Insulin; Insulin Resistance; Laboratories; Lipids; Metabolic; Modeling; National Institute of Diabetes and Digestive and Kidney Diseases; Non-Insulin-Dependent Diabetes Mellitus; Obesity; Periodicity; Play; Protocols documentation; Publications; Rattus; Relative (related person); Role; Role playing therapy; Sampling; Statistically Significant; Techniques; Time; Work; adipocyte differentiation; day; interest; size

This work was carried out in collaboration with the Cushman laboratory (Diabetes Branch, NIDDK) and Dr. Arthur Sherman (LBM, NIDDK). Obesity is often, but not always associated with insulin resistance. Insulin resistance is a fundamental characteristic of type II diabetes mellitus, even before the appearance of hyperglycemia, independent of the presence of obesity. An abnormality in adipose cell turnover has been postulated to play the unifying role in these metabolic relationships. To investigate the dynamics of adipose tissue, J. Mackellar in the Cushman laboratory developed a micro-biopsy protocol to sample adipose tissue from a rat at intervals of several days. Data was obtained under two conditions: normal and treated with thiazoledinediones. The normal animals showed adipose tissue dynamics on their own so our first goal was to mathematically characterize this development. Towards this end, we considered a variety of modeling approaches, and finally settled on computing the power spectrum of the cell size data. With some statistical analysis, we have found that there is a statistically significant periodicity in adipose tissue dynamics of about 40 days in normal untreated animals. This work is being prepared for publication. Our next goal is to use the same type of technique to characterize the changes in adipose tissue dynamics due to TZD treatment, relative to control.

这项工作是与Cushman实验室（NIDDK）和Arthur Sherman博士（LBM，NIDDK）合作进行的。 肥胖通常是，但并不总是与胰岛素抵抗有关。 胰岛素抵抗是II型糖尿病的基本特征，甚至在高血糖出现之前，与肥胖的存在无关。 据推测，脂肪细胞更新异常在这些代谢关系中起着统一的作用。 为了研究脂肪组织的动力学，库什曼实验室中的J. Mackellar开发了一种微型生物剖析方案，以几天的时间间隔从大鼠中采样脂肪组织。 数据是在两个条件下获得的：正常并用噻唑烷二酮处理。 正常动物自行表现出脂肪组织动力学，因此我们的第一个目标是数学上表征这种发展。 为此，我们考虑了各种建模方法，并最终确定计算单元尺寸数据的功率谱。 通过一些统计分析，我们发现正常未处理动物的脂肪组织动力学的统计学显着周期性在统计学上具有显着周期性。 这项工作正在准备出版。 我们的下一个目标是使用相同类型的技术来表征与对照相比，由于TZD处理而引起的脂肪组织动力学的变化。