Molecular Biology of Drosophila Learning and Memory

果蝇学习和记忆的分子生物学

• 批准号: 7365340
• 负责人: Ronald L Davis
• 金额: $ 33.58万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-12-01 至 2012-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7365340
• 关键词: Address; Adult; Alleles; Alzheimer' s Disease; Area; Autistic Disorder; Behavioral; Biological Assay; Brain; Calcium; Cells; Cyclic AMP; Drosophila genus; Functional Imaging; Genes; Genetic; Genetic screening method; Goals; Grant; Learning; Learning Disabilities; Lobe; Mediating; Memory; Molecular Biology; Molecular and Cellular Biology; Mushroom Bodies; Names; Nature; Nervous system structure; Neurons; Pathway Analysis; Pathway interactions; Pattern; Performance; Phenotype; Process; Protein Overexpression; Proteins; Research; Research Project Grants; Schizophrenia; Signal Transduction; Synapses; Synaptic Transmission; System; Techniques; Testing; Time; Training; cellular imaging; fly; mRNA Expression; member; mutant; nervous system disorder; new technology; protein expression; research study; tool; transgene expression

DESCRIPTION (provided by applicant): The long-term goals of this research project are to dissect the molecular and cellular biology of olfactory memory formation in Drosophila. A recent screen for new memory mutants with putative functions in the mushroom body neurons has uncovered several putative mutants, but one with a strong effect on performance immediately after training. Experiments are proposed to completely characterize the nature of the disrupted expression in different alleles of this gene, to reveal the spatial expression pattern of the gene within the olfactory nervous system, and to probe behaviorally whether the various alleles disrupt acquisition or memory stability. Genetic tests will reveal whether the mutant disrupts a molecule that is a member of well characterized signaling systems important for memory formation or whether this new gene represents the prototypic member of a parallel signaling system. Newly developed techniques to control transgene expression in time and in space will be used to address the issue of when and where the gene product is required for normal memory formation. Overall, the experiments will contribute to our understanding of the molecular biology of learning, an important process disrupted in many different diseases of the nervous system including Alzheimer's disease, schizophrenia, autism, and learning disabilities.

描述（由申请人提供）：该研究项目的长期目标是剖析果蝇中嗅觉记忆形成的分子和细胞生物学。最近在蘑菇体神经元中具有假定功能的新记忆突变体的屏幕发现了几个推定的突变体，但一个对训练后立即对性能产生了强烈影响。提出了实验以完全表征该基因不同等位基因中破坏表达的性质，以揭示嗅觉神经系统中基因的空间表达模式，并在行为上探测各种等位基因是否破坏了习得或记忆稳定性。基因测试将揭示突变体是否破坏了对记忆形成很重要的特征性信号系统成员的分子，还是该新基因代表并行信号系统的原型成员。新开发的技术来控制时间和空间中的转基因表达，以解决正常内存形成需要基因产物的何时何地的问题。总体而言，实验将有助于我们对学习分子生物学的理解，这是在神经系统的许多不同疾病中破坏的重要过程，包括阿尔茨海默氏病，精神分裂症，自闭症和学习障碍。