Regulation of APETALA2 in flower development in Arabidopsis

APETALA2 在拟南芥花发育中的调控

基本信息

批准号：
7540977
负责人：
Xuemei Chen
金额：
$ 27.24万
依托单位：
UNIVERSITY OF CALIFORNIA RIVERSIDE
依托单位国家：
美国
项目类别：
财政年份：
2000
资助国家：
美国
起止时间：
2000-05-01 至 2010-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7540977
关键词：
Address Animals Arabidopsis Biology Burn injury Cells Complex Development Developmental Process Family Flowers Funding Gene Expression Regulation Gene Targeting Genes Genetic Genetic Screening Goals Homeobox Genes Knowledge Lead Mediating Meristem Messenger RNA MicroRNAs Modeling Molecular Molecular Genetics Mouse-ear Cress Mustard Names Organ Organism Pathway interactions Pattern Plants Play Primordium Proteins RNA Regulation Repression Research Research Personnel Role Small RNA Specific qualifier value Therapeutic Agents Time Transcriptional Regulation Translational Repression Undifferentiated United States National Institutes of Health Work cell fate specification genetic analysis human disease insight interest member plant APETALA2 protein reproductive tool transcription factor

Address Animals Arabidopsis Biology Burn injury Cells Complex Development Developmental Process Family Flowers Funding Gene Expression Regulation Gene Targeting Genes Genetic Genetic Screening Goals Homeobox Genes Knowledge Lead Mediating Meristem Messenger RNA MicroRNAs Modeling Molecular Molecular Genetics Mouse-ear Cress Mustard Names Organ Organism Pathway interactions Pattern Plants Play Primordium Proteins RNA Regulation Repression Research Research Personnel Role Small RNA Specific qualifier value Therapeutic Agents Time Transcriptional Regulation Translational Repression Undifferentiated United States National Institutes of Health Work cell fate specification genetic analysis human disease insight interest member plant APETALA2 protein reproductive tool transcription factor

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项目摘要

We use flower development in Arabidopsis as a model to understand how cells in multicellular organisms assume their developmental fates and form distinct patterns. Our long-term goal is to identify and analyze the genetic networks and the underlying molecular mechanisms that lead to cell fate specification in the floral primordium. For a long time, three classes of floral homeotic transcription factors known as the A, B, and C genes have been the only players in floral organ identity specification. Our studies have identified a microRNA, miR172, as a translational represser of the class A gene APETALA2, indicating that posttranscriptional regulation also plays a role in flower development. In the proposed project, we will employ molecular genetic approaches to determine how miR172 fits in the regulatory circuitry governing flower development and to probe how miR172 regulates APETALA2 mRNA at the translational level. Homeotic genes that encode transcription factors act in cell fate specification in both animals and plants. Emerging evidence of microRNAs as regulators of homeotic genes in both animals and plants adds a new layer of regulation to the known transcriptional networks governing cell fate specification. The proposed research will undoubtedly provide insights into the integration of posttranscriptional and transcriptional mechanisms in developmental processes in multicellular organisms. The proposed research will also provide insights into the mechanism of microRNA-mediated translational repression of target mRNAs. Given the conserved actions of small RNAs in gene regulation in plants and animals and the potential of using small RNAs as therapeutic agents, this research will contribute to our understanding of small RNA biology in general and impact the uses of small RNAs to treat human diseases.

我们使用拟南芥中的花朵发育作为模型，以了解多细胞中的细胞如何生物体假定其发育命运并形成不同的模式。我们的长期目标是识别和分析导致的遗传网络和潜在的分子机制花卉原始中的细胞命运规范。长期以来，三类的花卉同源转录因子称为A，B和C 基因是花卉器官身份规范中唯一的玩家。我们的研究已经确定了 microRNA，mir172，作为A类基因apetala2的翻译阻遏物，表明转录后调节在花朵发育中也起作用。在拟议的项目中，我们将采用分子遗传方法来确定miR172如何拟合在调节电路中管理花的发育并探测miR172如何调节apetala2 mRNA 翻译水平。编码转录因子的同源基因在两种动物的细胞命运规范中作用和植物。 MicroRNA作为两种动物中同源基因的调节剂的新兴证据植物为控制细胞命运的已知转录网络添加了新的调节层规格。拟议的研究无疑将提供有关整合的见解多细胞的发育过程中的转录后和转录机制有机体。拟议的研究还将提供有关对机制机制的见解 microRNA介导的靶mRNA翻译抑制。考虑到保守的行动植物和动物中基因调节中的小rNA，以及使用小rNA作为治疗剂，这项研究将有助于我们对小RNA生物学的理解一般并影响小RNA治疗人类疾病的用途。