Eye Bank Cornea Screening with Optical Coherence Tomography

使用光学相干断层扫描进行眼库角膜筛查

• 批准号: 7659553
• 负责人: David Huang
• 金额: $ 21.21万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7659553
• 关键词: 3-Dimensional; Ablation; Algorithms; Anterior; Biological Preservation; Caliber; Characteristics; Cicatrix; Classification; Clinical; Computer software; Control Groups; Cornea; Corneal Stroma; Data; Detection; Diagnostic; Dimensions; Disease; Edema; Epithelial; Epithelium; Equipment; Eye; Eye Banks; Future; Human; Hyperopia; Image; Implant; International; Intraocular lens implant device; Keratoconus; Keratoplasty; Laser In Situ Keratomileusis; Lasers; Lead; Left; Maps; Measurement; Measures; Methodology; Methods; Microscope; Microscopic; Modification; Myopia; Operative Surgical Procedures; Optical Coherence Tomography; Optics; Outcome; Panthera leo; Pathology; Patients; Performance; Peripheral; Photorefractive Keratectomy; Procedures; Protocols documentation; Quality Indicator; Recording of previous events; Refractive Errors; Relative (related person); Research Personnel; Resolution; Scanning; Screening procedure; Speed; Staging; Sterility; Surface; Surgical Flaps; Surgical incisions; System; Techniques; Technology; Testing; Thick; Three-Dimensional Imaging; Time; Tissue Banking; Tissue Banks; Tissue Transplantation; Tissues; Transplantation; Width; Work; base; computer program; corneal surgery; cost; digital; digital imaging; image processing; improved; programs; prototype; success; tool; wound

DESCRIPTION (provided by applicant): The transplantation of donor cornea that has undergone refractive surgery (LASIK, RK, PRK) can lead to both wound separation during surgery and to increased refractive error in the recipient. Therefore, the increasing popularity of refractive surgery has raised concern regarding the decreasing proportion of transplant quality corneas coming in to the eye bank and the serious consequences of transplanting a post- refractive surgery cornea that has escaped detection. Currently, eye banks utilize patient history and microscopic examination to assess the quality of donor corneas and detect previous refractive surgery. Although these methods are capable of screening out many problem corneas, they are subject to human error. Patient history may be unreliable, and well-performed LASIK and PRK may leave minimal debris and scarring and which are particularly difficult to detect. Optical coherence tomography (OCT) is capable of performing 3-dimensional imaging of the cornea with micron resolution. It can visualize the corneal layers, detect opacities, and measure surface contours. In our preliminary studies, we have found that OCT can image donor corneal tissue without even removing it from the sterile container and preservation medium. The digital OCT data is amenable to automated image processing. These characteristics are ideal for a screening test. Therefore, our specific aims are to: 1. Develop a high performance OCT system for eye bank screening. Using the ultra-highspeed Fourier- domain OCT technology, the system will provide detailed 3-D scanning within 3 minutes. 2. Develop OCT to detect previous refractive surgery. Our preliminary data showed that OCT can detect topographic and reflectivity changes in the post-LASIK cornea. We will develop image processing algorithms to detect LASIK, PRK, RK, and any surgery that modifies corneal curvature. 3. Develop OCT to assess donor corneal quality. We will develop criteria to automatically quantify corneal quality indicators: topographic regularity, epithelial sloughing, stromal opacity/edema, and endothelial striae. 4. Develop OCT to measure donor cornea power to improve refractive outcome of transplant surgery. We will develop and program the OCT system and place it at the Lions Doheny Eye Bank to screen all corneal tissues. Tissue Bank International will forward known post-refractive surgery and diseased corneas. Years 1-2 will be used to develop the OCT system and image process software. Years 3-4 will be used to assess the performance of automated OCT donor cornea classification and refine the software. If successful, we will safeguard the future supply of donor corneal tissue for transplantation by offering an objective and reliable screening method that adds minimal time and cost to eye bank procedures..

描述（由申请人提供）：接受屈光手术（LASIK、RK、PRK）的供体角膜移植可能会导致手术期间伤口分离，并导致受者屈光不正增加。因此，屈光手术的日益普及引起了人们对进入眼库的移植优质角膜的比例下降以及移植漏检的屈光手术后角膜的严重后果的担忧。目前，眼库利用患者病史和显微镜检查来评估供体角膜的质量并检测以前的屈光手术。尽管这些方法能够筛查出许多有问题的角膜，但它们可能会出现人为错误。患者病史可能不可靠，而执行良好的 LASIK 和 PRK 可能会留下极少的碎片和疤痕，而且特别难以检测。光学相干断层扫描 (OCT) 能够以微米分辨率对角膜进行 3 维成像。它可以可视化角膜层、检测混浊度并测量表面轮廓。在我们的初步研究中，我们发现 OCT 可以对供体角膜组织进行成像，甚至无需将其从无菌容器和保存介质中取出。数字 OCT 数据适合自动图像处理。这些特征非常适合筛选测试。因此，我们的具体目标是： 1. 开发用于眼库筛查的高性能 OCT 系统。利用超高速傅里叶域OCT技术，系统将在3分钟内提供详细的3D扫描。 2. 开发 OCT 来检测既往屈光手术。我们的初步数据表明，OCT 可以检测 LASIK 术后角膜的地形和反射率变化。我们将开发图像处理算法来检测 LASIK、PRK、RK 以及任何改变角膜曲率的手术。 3. 开发 OCT 来评估供体角膜质量。我们将制定自动量化角膜质量指标的标准：地形规律性、上皮脱落、基质混浊/水肿和内皮纹。 4. 开发OCT测量供体角膜屈光力，以改善移植手术的屈光结果。我们将开发和编程 OCT 系统，并将其放置在 Lions Doheny 眼库中以筛查所有角膜组织。国际组织银行将转发已知的屈光手术后和患病的角膜。第 1-2 年将用于开发 OCT 系统和图像处理软件。第 3-4 年将用于评估自动 OCT 供体角膜分类的性能并完善软件。如果成功，我们将通过提供客观可靠的筛选方法来保障未来用于移植的供体角膜组织的供应，从而最大限度地减少眼库程序的时间和成本。