Mass Spec Resource

质谱资源

• 批准号: 7645376
• 负责人: STEPHEN B. CALDERWOOD
• 金额: $ 17.75万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7645376
• 关键词: Applied Research; Charge; Communication; Development; Doctor of Medicine; Isotope Labeling; Mails; Microbe; Mission; National Institute of Allergy and Infectious Disease; New England; Peptides; Proteins; Research Personnel; Resolution; Resources; Scanning; Work; antimicrobial; inorganic phosphate; instrument; mass spectrometer; pathogen

NERCE: Mass Spec Resource - Stephen Calderwood, M.D. The LTQ XL is a fast scanning tandem mass spectrometer, capable of sequencing several peptides every second, allowing rapid characterization and quantification (using isotope labels) of proteins from microbes. The instrument also has several intermediate scan modes, allowing for neutral loss analysis of phosphate groups, as well as some medium full scan resolution modes for charge state assessment. The instrument has a higher duty cycle than the LTQ-FT, and a higher sensitivity for lower level peptides. Use of this instrument is prioritized for New England investigators working on NIAID Priority Pathogens, for which rapid identification of proteins and peptides will facilitate basic and applied science of relevant to the missions of NERCE, such as the identification of pathogen proteins that are potential targets for antimicrobial development. This resource is publicized to NERCE investigators via e-mail, the NERCE Website, and direct communication when appropriate.

Nerce：Mass Spec资源 - Stephen Calderwood，医学博士 LTQ XL是快速扫描串联质谱仪，能够测序多个肽 每一秒，都可以快速表征和定量（使用同位素标签） 微生物。该仪器还具有几种中间扫描模式，可以进行中性损失分析 磷酸盐基团以及一些中等的完整扫描分辨率模式进行电荷状态评估。这 仪器的占空比比LTQ-ft更高，对较低肽的敏感性更高。 使用此工具的使用优先级，用于从事NIAID优先病原体的新英格兰调查人员的使用， 为此快速鉴定蛋白质和肽将有助于与 尼尔的任务，例如鉴定病原体蛋白，这些蛋白是潜在的靶标 抗菌发育。该资源通过电子邮件（Nerce）向Nerce调查人员公开 网站，并在适当的情况下进行直接沟通。