ORAL TRANSMISSION OF SIV IN NEONATAL AND ADULT MACAQUES

新生儿和成年猕猴中 SIV 的口腔传播

• 批准号: 7562017
• 负责人: Shawn Patrick O'Neil
• 金额: $ 20.75万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7562017
• 关键词: Adult; Cells; Childhood; Computer Retrieval of Information on Scientific Projects Database; Exposure to; Funding; Gastrointestinal tract structure; Grant; HIV; HIV Infections; Human Milk; Immunohistochemistry; In Situ Hybridization; Infection; Ingestion; Institution; Macaca; Methods; Modeling; Monkeys; Mucous Membrane; Neonatal; Nested PCR; Oral; Pattern; Research; Research Personnel; Resources; Reverse Transcriptase Polymerase Chain Reaction; Route; SIV; Source; Subfamily lentivirinae; United States National Institutes of Health; Viral; Virus; cellular targeting; day; transmission process; viral RNA

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Ingestion of HIV contaminated breast milk is a major route of pediatric HIV infection; however, the portals of viral entry, patterns of virus dissemination, and early target cells following oral exposure to HIV have not been identified. We are using the simian immunodeficiency virus (SIV) model to investigate the mechanism of lentivirus transmission across the mucosa of the alimentary tract in neonatal and adult monkeys after oral exposure. In this model, macaques are inoculated with SIV by the oral route, and the mucosal portals and cellular targets of infection are identified during the first days following exposure by nested PCR, RT-PCR and histotechnical methods (in situ hybridization for SIV RNA and immunohistochemistry for SIVp27).

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 摄入HIV污染的母乳是小儿艾滋病毒感染的主要途径；但是，尚未鉴定出病毒进入的门户，病毒传播模式和口腔接触HIV后的早期靶细胞。我们正在使用猿猴免疫缺陷病毒（SIV）模型来研究口腔暴露后新生儿和成年猴子慢跑病毒传播的机理。 在此模型中，猕猴通过口腔途径接种SIV，在暴露后的第一天通过嵌套的PCR，RT-PCR和组织技术方法（SIV RNA的原位杂交用于SIV RNA和SIVP27的免疫组织），粘膜门户和感染的细胞靶标被鉴定出来。