A Molecular Genetic Analysis of Root Morphogenesis

根形态发生的分子遗传学分析

基本信息

批准号：
7529878
负责人：
Philip N Benfey
金额：
$ 32.9万
依托单位：
DUKE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1991
资助国家：
美国
起止时间：
1991-01-01 至 2009-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7529878
关键词：
Agriculture Animals Arabidopsis Binding Biotechnology Cell Separation Cell division Cells Congenital Abnormality Data Set Development Developmental Process Ectopic Expression Electrophoretic Mobility Shift Assay Elements Embryonic Development Fluorescence Foundations Gel Gene Targeting Genes Genetic Screening Genetic Transcription Genomics Goals Health Hybrids Link Malignant Neoplasms Mediating Meta-Analysis Methods Microarray Analysis Molecular Molecular Genetics Morphogenesis Movement Nucleic Acid Regulatory Sequences Organ Pathway interactions Pattern Plant Roots Plants Proteins Radial Regulation Regulatory Element Research Research Personnel Signal Pathway Structure System Testing Tissues Translating Two-Hybrid System Techniques Work Yeasts chromatin immunoprecipitation deletion analysis genetic analysis in vivo insight loss of function mutation mutant novel promoter protein expression transcription factor yeast two hybrid system

项目摘要

The long-term goal of the proposed research is to understand the developmental processes that regulate organ formation. Current work focuses on the radial organization of the Arabidopsis root as a simple and tractable system. A novel signaling pathway has been discovered that links two key players in radial patterning, SHORT-ROOT (SHR)and SCARECROW (SCR). SHR is translated in the stele and then moves to the adjacent cell layer where it activates SCR transcription and initiates endodermal specification. SCR, possibly in concert with SHR,regulates the asymmetric cell division that results in the formation of cortex and endodermis. Preliminary results indicate that SHR interacts directly with the SCR promoter. A meta- analysis of results from genomics approaches identified other candidate targets of SHR that may be involved in cell division and cell specification. Domains and residues within the SHR protein have been identified that are required for movement and activity. Building on this foundation, several hypotheses relating to this central pathway in root development will be tested. A structure/function analysis and yeast two-hybrid approach will be used to test the hypothesis that SHR movement and activity are mediated by interaction of specific domains in the SHR protein with other factors. Genomics approaches will be used to test the second hypothesis that SHR regulates asymmetric cell division and cell specification through a transcription factor cascade. A genetic screen and the yeast one-hybrid method will be used to test the third hypothesis that the SHR-mediated pathway is initiated in embryogenesis and maintained during development by transcription factors that are expressed in a tissue-specific manner. Specific aims are to: 1) Elucidate the mechanisms regulating SHR movement and activity; 2) Determine how the SHR-mediated signaling pathway controls cell specification and asymmetric cell division: 3) Identify the factors that initiate and maintain the SHR-mediated signaling pathway. The picture that is emerging of the SHR-mediated regulation of radial patterning has some clear similarities and apparent differences with pathways regulating animal organogenesis.Comparing and contrasting plant and animal development can provide important insights into both, leading to a better understanding of developmental^ related health concerns such as birth defects and cancer as well as improvements in biotechnology and agriculture.

拟议研究的长期目标是了解调节的发育过程器官形成。目前的工作重点是拟南芥根的放射状组织作为一种简单且易于处理的系统。发现了一种新的信号通路，将径向的两个关键参与者联系起来图案、短根 (SHR) 和稻草人 (SCR)。 SHR 在石碑中被翻译，然后移动到邻近的细胞层，在那里它激活 SCR 转录并启动内胚层规范。可控硅，可能与 SHR 协同作用，调节导致皮质形成的不对称细胞分裂和内皮层。初步结果表明 SHR 直接与 SCR 启动子相互作用。一个元基因组学方法的结果分析确定了 SHR 的其他候选目标，这些目标可能是参与细胞分裂和细胞分化。 SHR 蛋白内的结构域和残基已被确定运动和活动所需的内容。在此基础上提出几个假设与根发育的这一中心途径相关的部分将受到测试。结构/功能分析和酵母两种混合方法将用于检验 SHR 运动和活动由以下因素介导的假设： SHR 蛋白中特定结构域与其他因子的相互作用。基因组学方法将用于检验第二个假设，即 SHR 通过以下方式调节不对称细胞分裂和细胞分化：转录因子级联。将使用遗传筛选和酵母单杂交方法来测试第三种假设 SHR 介导的途径在胚胎发生中启动并在胚胎发生过程中维持由以组织特异性方式表达的转录因子发育。具体目标是：1) 阐明调节 SHR 运动和活动的机制； 2）确定SHR如何介导信号通路控制细胞规格和不对称细胞分裂：3) 识别启动因素并维持 SHR 介导的信号通路。以 SHR 为媒介的图景正在浮现放射状图案的调节与途径调节有一些明显的相似之处和明显的差异动物器官发生。比较和对比植物和动物的发育可以提供重要的信息对两者的深入了解，可以更好地理解与发育相关的健康问题，例如出生缺陷和癌症以及生物技术和农业的进步。