A Molecular Genetic Analysis of Root Morphogenesis

根形态发生的分子遗传学分析

• 批准号: 7529878
• 负责人: Philip N Benfey
• 金额: $ 32.9万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-01-01 至 2009-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7529878
• 关键词: Agriculture; Animals; Arabidopsis; Binding; Biotechnology; Cell Separation; Cell division; Cells; Congenital Abnormality; Data Set; Development; Developmental Process; Ectopic Expression; Electrophoretic Mobility Shift Assay; Elements; Embryonic Development; Fluorescence; Foundations; Gel; Gene Targeting; Genes; Genetic Screening; Genetic Transcription; Genomics; Goals; Health; Hybrids; Link; Malignant Neoplasms; Mediating; Meta-Analysis; Methods; Microarray Analysis; Molecular; Molecular Genetics; Morphogenesis; Movement; Nucleic Acid Regulatory Sequences; Organ; Pathway interactions; Pattern; Plant Roots; Plants; Proteins; Radial; Regulation; Regulatory Element; Research; Research Personnel; Signal Pathway; Structure; System; Testing; Tissues; Translating; Two-Hybrid System Techniques; Work; Yeasts; chromatin immunoprecipitation; deletion analysis; genetic analysis; in vivo; insight; loss of function mutation; mutant; novel; promoter; protein expression; transcription factor; yeast two hybrid system

The long-term goal of the proposed research is to understand the developmental processes that regulate organ formation. Current work focuses on the radial organization of the Arabidopsis root as a simple and tractable system. A novel signaling pathway has been discovered that links two key players in radial patterning, SHORT-ROOT (SHR)and SCARECROW (SCR). SHR is translated in the stele and then moves to the adjacent cell layer where it activates SCR transcription and initiates endodermal specification. SCR, possibly in concert with SHR,regulates the asymmetric cell division that results in the formation of cortex and endodermis. Preliminary results indicate that SHR interacts directly with the SCR promoter. A meta- analysis of results from genomics approaches identified other candidate targets of SHR that may be involved in cell division and cell specification. Domains and residues within the SHR protein have been identified that are required for movement and activity. Building on this foundation, several hypotheses relating to this central pathway in root development will be tested. A structure/function analysis and yeast two-hybrid approach will be used to test the hypothesis that SHR movement and activity are mediated by interaction of specific domains in the SHR protein with other factors. Genomics approaches will be used to test the second hypothesis that SHR regulates asymmetric cell division and cell specification through a transcription factor cascade. A genetic screen and the yeast one-hybrid method will be used to test the third hypothesis that the SHR-mediated pathway is initiated in embryogenesis and maintained during development by transcription factors that are expressed in a tissue-specific manner. Specific aims are to: 1) Elucidate the mechanisms regulating SHR movement and activity; 2) Determine how the SHR-mediated signaling pathway controls cell specification and asymmetric cell division: 3) Identify the factors that initiate and maintain the SHR-mediated signaling pathway. The picture that is emerging of the SHR-mediated regulation of radial patterning has some clear similarities and apparent differences with pathways regulating animal organogenesis.Comparing and contrasting plant and animal development can provide important insights into both, leading to a better understanding of developmental^ related health concerns such as birth defects and cancer as well as improvements in biotechnology and agriculture.

拟议研究的长期目标是了解规范的发展过程 器官形成。当前的工作重点是拟南芥根的径向组织 可处理系统。已经发现了一个新颖的信号通路，它链接了径向的两个关键参与者 图案，短根（SHR）和稻草人（SCR）。 SHR在石碑中翻译，然后移动 到相邻的细胞层，该层激活SCR转录并启动内胚层规范。 scr， 可能与SHR协同调节不对称的细胞分裂，导致皮质形成 和内胚层。初步结果表明SHR直接与SCR启动子相互作用。元 - 基因组学方法的结果分析可能是SHR的其他候选靶标的 参与细胞分裂和细胞规范。 SHR蛋白中的域和残基 确定运动和活动所需的。在这个基础的基础上，几个假设 与该根部发育中的这一中心途径有关。结构/功能分析和酵母 两种杂交方法将用于检验以下假设：SHR运动和活动是由 SHR蛋白中特定结构域与其他因素的相互作用。基因组学方法将用于 测试SHR通过A调节不对称细胞分裂和细胞规范的第二个假设 转录因子级联。遗传屏幕和酵母单杂交方法将用于测试第三种 假设SHR介导的途径是在胚胎发生中启动的，并在 通过以组织特异性方式表达的转录因子的发展。具体目的是：1） 阐明调节SHR运动和活性的机制； 2）确定SHR介导的如何 信号通路控制细胞规范和不对称细胞分裂：3）确定启动的因素 并保持SHR介导的信号通路。 SHR介导的图片 径向模式的调节具有一些明显的相似性和明显的差异与调节的途径 动物器官发生。 对两者的见解，从而更好地了解与发展相关的健康问题，例如出生 缺陷和癌症以及生物技术和农业的改善。