Identification of the 1-methyladenine receptor

1-甲基腺嘌呤受体的鉴定

• 批准号: 7317815
• 负责人: Sean P Place
• 金额: $ 4.96万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA BARBARA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7317815
• 关键词: 1-methyladenine; 1-methyladenine receptor; Antisense Oligonucleotides; Binding; Biochemical; Biological Models; Cell Cycle; Competence; Complementary DNA; Fertilization; GTP-Binding Proteins; Goals; Hormone Receptor; Hormones; Knowledge; Laboratories; Link; Mammals; Molecular; Oocytes; Process; Property; Proteins; Research Design; Signal Pathway; Signal Transduction; Starfish; System; Testing; Work; analog; base; concept; design; expression cloning; novel strategies; oocyte maturation; prevent; receptor; research study; 1-methyladenine; 1-methyladenine receptor; Antisense Oligonucleotides; Binding; Biochemical; Biological Models; Cell Cycle; Competence; Complementary DNA; Fertilization; GTP-Binding Proteins; Goals; Hormone Receptor; Hormones; Knowledge; Laboratories; Link; Mammals; Molecular; Oocytes; Process; Property; Proteins; Research Design; Signal Pathway; Signal Transduction; Starfish; System; Testing; Work; analog; base; concept; design; expression cloning; novel strategies; oocyte maturation; prevent; receptor; research study

The goal of the proposed study is to identify and characterize the oocyte maturation hormone receptor in the starfish, Patina miniata. This model system offers signficant advantages over others and some aspects may be shared with mammalian oocytes. The proposed work is designed to identify and characterize this receptor using unique homology based and functional expression cloning based approaches in tandem to identify and clone candidate cDNA(s) encoding the receptor for the maturation-inducing hormone, 1-methyladenine (1- MA). Successful identification of the 1-MA receptor using these novel strategies would represent the first definitive description of a maturation-inducing hormone receptor in any species and provide proof of concept for the strategy to be used for the identification of maturation hormone receptors in other species, including mammals. Several candidate cDNAs already have been isolated; these and additional candidates will be subjected to further functional studies to confirm identity and tests for necessity in oocyte maturation will be conducted. Eventually, studies will be conducted that are designed to elucidate the details of the molecular mechanism of signal transduction during oocyte maturation.

拟议的研究的目的是识别和表征卵母细胞成熟的激素受体 海星，Patina Miniata。该模型系统比其他系统具有显着优势，某些方面可能 与哺乳动物卵母细胞共享。拟议的工作旨在识别和表征该受体 使用基于唯一同源性和功能表达克隆的基于克隆的方法来识别和 克隆候选cDNA（S）编码成熟激素的受体，1-甲基二氨酸（1- 马）。使用这些新颖策略成功识别1-MA受体将代表第一个 在任何物种中诱导成熟激素受体的确定描述，并提供概念证明 为了用于鉴定其他物种的成熟激素受体，包括 哺乳动物。已经隔离了几个候选cDNA。这些和其他候选人将是 进行进一步的功能研究以确认身份和测试卵母细胞成熟的必要性 实施。最终，将进行旨在阐明分子细节的研究。 卵母细胞成熟过程中信号转导的机理。