Markers of sensivity to the EGFR inhibitor erlotinib in breast cancer

乳腺癌中 EGFR 抑制剂厄洛替尼敏感的标志物

• 批准号: 7630446
• 负责人: Naoto T Ueno
• 金额: $ 29.26万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-05 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7630446
• 关键词: Affect; Apoptosis; Binding; Biological; Biological Assay; Biological Markers; Breast; Breast Cancer Cell; Bromodeoxyuridine; Cancer Biology; Cancer Patient; Cell Cycle; Cell Nucleus; Cell Proliferation; Cells; Clinical; Community Clinical Oncology Program; Cytoplasm; Development; Diagnostic; Elements; Epidermal Growth Factor; Epidermal Growth Factor Receptor; Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitor; Erlotinib; Freezing; G1 Phase; Gefitinib; Genetic Transcription; Goals; Growth Factor Receptors; Immunoblotting; In Vitro; Knowledge; Luc Gene; Lung; Malignant neoplasm of pancreas; Measures; Mediating; Medical Oncologist; Methods; Modeling; Molecular; Molecular Biology; Molecular Mechanisms of Action; Northern Blotting; Nuclear; Outcome; Paraffin Embedding; Pathologist; Pathway interactions; Patient Selection; Patients; Pattern; Phase; Phosphoproteins; Phosphorylation; Phosphorylation Site; Phosphotransferases; Protein Tyrosine Kinase; Proteins; Receptor Signaling; Recruitment Activity; Research; Resistance; Role; Sampling; Scanning; Signal Pathway; Signal Transduction; Small Interfering RNA; Soft Agar Assay; Subgroup; Techniques; Threonine; Tissue Sample; Training; Translating; Tyrosine Kinase Inhibitor; Ubiquitination; Work; Xenograft Model; base; cancer therapy; cell growth; clinical efficacy; clinically relevant; cytotoxicity; design; experience; human CDK2 protein; improved; in vitro activity; in vivo; insight; knock-down; malignant breast neoplasm; mutant; novel; novel therapeutic intervention; overexpression; promoter; protein degradation; protein profiling; resistance mechanism; response; tool; tumor

DESCRIPTION (provided by applicant): The epidermal growth factor receptor (EGFR) signaling pathway is a central regulator of cell growth and proliferation and modulates critical cell cycle regulatory molecules. This pathway has emerged as a promising target for cancer therapy. EGFR tyrosine kinase inhibitors (TKIs), such as erlotinib (Tarceva) and gefitinib (Iressa), are approved for cancer treatment but have induced a clinical response in only a subgroup of patients. Therefore, EGFR-TKI's molecular mechanism of action needs to be better understood. My long-term goal is to elucidate the molecular mechanism of action of EGFR-TKI so that novel therapeutic approaches or diagnostic tools that are clinically relevant can be developed. I have recently shown that in vitro erlotinib sensitivity is partially dependent on the activity of cyclin-dependent kinase 2 (Cdk2), which is the most downstream kinase of the EGFR pathway that regulates the transition from the G1 phase to the S phase. The objective of this application is to determine which downstream molecules of the EGFR and non-EGFR signaling pathway predict the response to EGFR-TKIs. The central hypothesis of this proposal is that the effect of Cdk2 activity on EGFR-TKI-mediated cytotoxicity is regulated by downstream molecules of the cell signaling pathway, specifically ERK, p27, and PEA15. This hypothesis is based on the following observations. First, erlotinib inhibits the tyrosine kinase of EGFR in both erlotinib-sensitive and erlotinib-resistant breast cancer cells; however, erlotinib inhibits Cdk2 activity only in sensitive cells. These findings indicate that there is an abnormality in the EGFR signaling pathway in erlotinib-resistant cells. Second, phosphorylated ERK is downregulated and p27 is upregulated in erlotinib-sensitive cells, and the phosphorylation status of p27 affects its nuclear-cytoplasmic localization and expression level. Third, PEA15 sequesters ERK into the cytoplasm from the nucleus and reduces cell proliferation. I have designed three independent but interrelated specific aims to provide a comprehensive assessment of the downstream EGFR and non-EGFR signaling pathway in breast cancer cells treated with EGFR-TKI. Specific Aim 1. Establish how erlotinib regulates p27 to suppress Cdk2 activity in vitro. Specific Aim 2. Establish how PEA15 modulates erlotinib sensitivity in vitro and in vivo. Specific Aim 3. Establish in vivo biomarkers that predict erlotinib sensitivity. Relevance: This study will provide significant insight into the role of downstream molecules affected by EGFR-TKI. Our findings will improve the outcome of cancer patients by increasing EGFR-TKI efficacy and facilitating the selection of patients who may benefit from EGFR-TKI therapy.

描述（由申请人提供）：表皮生长因子受体（EGFR）信号通路是细胞生长和增殖的中心调节因子，并调节关键的细胞周期调节分子。该通路已成为癌症治疗的一个有前景的靶点。 EGFR 酪氨酸激酶抑制剂 (TKI)，例如厄洛替尼 (Tarceva) 和吉非替尼 (Iressa)，已被批准用于癌症治疗，但仅在一小部分患者中引起临床反应。因此，需要更好地了解EGFR-TKI的分子作用机制。我的长期目标是阐明 EGFR-TKI 的分子作用机制，以便开发出临床相关的新型治疗方法或诊断工具。我最近表明，体外厄洛替尼敏感性部分依赖于细胞周期蛋白依赖性激酶 2 (Cdk2) 的活性，Cdk2 是 EGFR 通路的最下游激酶，调节从 G1 期到 S 期的转变。本申请的目的是确定 EGFR 和非 EGFR 信号通路的哪些下游分子可预测对 EGFR-TKI 的反应。该提议的中心假设是，Cdk2 活性对 EGFR-TKI 介导的细胞毒性的影响受到细胞信号通路下游分子（特别是 ERK、p27 和 PEA15）的调节。该假设基于以下观察。首先，厄洛替尼在厄洛替尼敏感和厄洛替尼耐药的乳腺癌细胞中抑制EGFR的酪氨酸激酶；然而，厄洛替尼仅在敏感细胞中抑制 Cdk2 活性。这些发现表明厄洛替尼耐药细胞中的 EGFR 信号通路存在异常。其次，在厄洛替尼敏感细胞中，磷酸化的ERK下调，p27上调，p27的磷酸化状态影响其核质定位和表达水平。第三，PEA15将ERK从细胞核隔离到细胞质中并减少细胞增殖。我设计了三个独立但相互关联的具体目标，以对接受 EGFR-TKI 治疗的乳腺癌细胞中的下游 EGFR 和非 EGFR 信号通路进行全面评估。具体目标 1. 确定厄洛替尼如何在体外调节 p27 抑制 Cdk2 活性。具体目标 2. 确定 PEA15 如何在体外和体内调节厄洛替尼敏感性。具体目标 3. 建立预测厄洛替尼敏感性的体内生物标志物。相关性：本研究将深入了解受 EGFR-TKI 影响的下游分子的作用。我们的研究结果将通过提高 EGFR-TKI 疗效并促进选择可能受益于 EGFR-TKI 治疗的患者来改善癌症患者的预后。