Georgia Comprehensive Metabolomics and Proteomics Unit for MoTrPAC

佐治亚州 MoTrPAC 综合代谢组学和蛋白质组学单位

基本信息

批准号：
9246760
负责人：
Dean Paul Jones
金额：
$ 8.45万
依托单位：
EMORY UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-12-08 至 2022-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9246760
关键词：
3-Dimensional Adipocytes Affect Aging Animal Experimentation Animals Automobile Driving Big Data Bioinformatics Biological Assay Chemicals Clinical Clinical Research Clinical Sciences Collaborations Communication Complement Complex Custom Data Data Coordinating Center Development Dimensions Disease Educational workshop Elements Environment Exercise Forensic Medicine Goals Health Health Benefit Immunoassay Institutes Isotopes Label Laboratories Leadership Link Lipids Liquid Chromatography Machine Learning Malignant Neoplasms Maps Mass Spectrum Analysis Measurement Measures Mediating Methods Modality Molecular Molecular Structure Molecular Target Monitor Muscle NMR Spectroscopy Neurodegenerative Disorders Nuclear Oral Oxidation-Reduction Oxidative Stress Particle Size Peptide Mapping Phosphotransferases Physical activity Plasma Post-Translational Protein Processing Procedures Process Proteins Proteome Proteomics Protocols documentation Publications Reaction Records Research Research Personnel Resolution Sampling Scientist Signal Transduction Site System Systems Biology Technology Time Training Transducers Translational Research United States National Institutes of Health Universities Validation advanced system analytical method chemoproteomics cytokine data resource design differential expression innovation instrumentation ion mobility lectures member metabolomics molecular imaging novel oxidized lipid phenotypic biomarker response small molecule tandem mass spectrometry tool ultra high resolution

项目摘要

PROJECT SUMMARY The Georgia Comprehensive Metabolomics and Proteomics Unit for MoTrPAC (CMP-GA) is a cross-cutting team with advanced high-throughput chemical analytics and big data capabilities to develop a comprehensive map of molecular transducers of physical activity. The investigative team excels in ultrasensitive, precise and spatially resolved analyses of small molecules, lipids, and proteins. The team members have strong academic records as innovative, independent scientists, core laboratory directors and effective collaborators in complex research initiatives. Instrumentation includes state-of-the-art ultra-high resolution accurate mass high-field Orbitrap tandem mass spectrometry (MS) and ultraperformance liquid chromatography (UPLC); three- dimensional (3-D) molecular imaging via high throughput multi-modal MS at 5 m resolution; unique ion mobility-mass spectrometry machine-learning approaches for chemical identifications; and other such as GC- Orbitrap, 1D and 2D high field (800 MHz) NMR spectroscopy, ICP-MS, immunoassays, chemical and enzymatic assays, etc. The analytical laboratories are integrated through the NIH-supported Atlanta Clinical and Translational Sciences Institute, and operate within the outstanding research environment of Emory University and the Georgia Institute of Technology (GA Tech). CMP-GA has six elements organized to provide 1) leadership in the design and implementation of MoTrPAC goals; 2) bioinformatics, computational support and data delivery to the MoTrPAC Data Coordinating Center; 3) global, targeted and spatially resolved metabolomics; 4) metabolite forensics for unequivocal chemical identification of novel molecular transducers; 5) innovative proteomic and chemoproteomic technologies to define transducers within the redox proteome, ubiquitinome, acetylome, kinome and nuclear proteome; and 6) identification and quantification of lipid transducers. Through the successful completion of these aims and collaboration with the MoTrPAC consortium, investigators of CMP-GA will deliver a publically-available data resource and molecular transducer map that will enhance and accelerate mechanistic research on diseases and conditions affected by physical activity.

项目概要佐治亚州 MoTrPAC 综合代谢组学和蛋白质组学单位 (CMP-GA) 是一个跨领域的机构团队拥有先进的高通量化学分析和大数据能力，开发全面的身体活动的分子传感器图谱研究团队擅长超灵敏、精确和精确。小分子、脂质、蛋白质的空间分辨分析，团队成员学术实力雄厚。记录作为复杂的创新、独立科学家、核心实验室主任和有效合作者研究计划包括最先进的超高分辨率精确质量高场。 Orbitrap串联质谱（MS）和超高效液相色谱（UPLC）；通过高通量多模态 MS 以 5 µm 分辨率进行三维 (3-D) 分子成像；用于化学鉴定的迁移率质谱机器学习方法以及其他方法，例如 GC- Orbitrap、一维和二维高场 (800 MHz) NMR 波谱、ICP-MS、免疫测定、化学和酶分析等。分析实验室通过 NIH 支持的亚特兰大临床中心进行整合和转化科学研究所，并在埃默里大学出色的研究环境中运作大学和佐治亚理工学院 (GA Tech) 有六个组织机构提供服务。 1) 领导 MoTrPAC 目标的设计和实施 2) 生物信息学、计算支持；并将数据传送至 MoTrPAC 数据协调中心 3) 全球、有针对性和空间解析；代谢组学；4）代谢物取证，用于新型分子传感器的明确化学鉴定； 5) 创新的蛋白质组学和化学蛋白质组学技术，用于定义氧化还原蛋白质组内的传感器，泛素组、乙酰组、激酶组和核蛋白质组；6) 脂质的鉴定和定量；通过成功完成这些目标以及与 MoTrPAC 的合作。 CMP-GA 联盟的研究人员将提供公开可用的数据资源和分子传感器该地图将加强和加速对受物理影响的疾病和状况的机制研究活动。