Effects of chronic use of thickened liquids in a rat model of aging

长期使用增稠液体对衰老大鼠模型的影响

• 批准号: 10728328
• 负责人: Linda M Rowe
• 金额: $ 4.72万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-07 至 2024-09-06
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10728328
• 关键词: Address; Affect; Age; Aging; Animal Model; Aspiration Pneumonia; Behavioral Assay; Benefits and Risks; Biological; Biological Assay; Biology; Blood specimen; Bolus Infusion; Cephalic; Chronic; Clinical; Clinical Research; Clinical Treatment; Contracts; Deglutition; Deglutition Disorders; Dehydration; Disease; Dose; Elderly; Equilibrium; Event; Exposure to; Fast-Twitch Muscle Fibers; Food; Foundations; Frequencies; Future; Goals; Guidelines; Health; Health care facility; Hematocrit procedure; Hydration status; Individual; Institute of Medicine (U.S.); Intake; Intervention; Invaded; Knowledge; Liquid substance; Long-Term Effects; Malnutrition; Measurement; Measures; Medical; Methods; Modeling; Monitor; Movement; Muscle; Muscle Contraction; Myosin Heavy Chains; Nonmuscle Myosin Type IIB; Oral; Oropharyngeal Dysphagia; Outcome; Outcome Measure; Patient risk; Patients; Persons; Physiology; Population; Property; Protein Isoforms; Quality of life; Rattus; Recommendation; Research; Risk; Solid; Speed; Stimulus; System; Testing; Texture; Thinness; Time; Tissues; Tongue; Translations; Urinary tract infection; Viscosity; Work; age effect; age related; aging population; aspirate; clinical decision-making; clinically relevant; compliance behavior; genioglossus muscle; improved; insight; kinematics; medical complication; mortality; nutrition; prevent; respiratory; response; young adult

Abstract Swallowing disorders are highly prevalent in elderly people, including those with age-related diseases. Thickened liquids are frequently used in the treatment of swallowing disorders and have been shown to immediately reduce the chance of airway invasion. However, thickened liquids have been associated with deleterious health effects such as dehydration, urinary tract infection, and reduced quality of life. Despite their common clinical use, the long-term efficacy of thickened liquids, and their effect on swallowing kinematics, systemic hydration, and underlying biology, is not well understood. Given the rapidly increasing population of persons over 65, estimated to reach 2.1 billion by 2051, it is critical to understand the efficacy of dysphagia interventions. The overarching goal of this proposal is to contribute foundational knowledge of biological mechanisms underlying functional swallowing changes in response to a frequently used, but understudied, intervention for dysphagia management in the aging population. The proposed study will use an aging rat model to interrogate how use of thickened liquids affects swallowing kinematics, systemic hydration, and underlying biology over time. We have 3 specific aims, each with a unique hypothesis. Aim 1 will test the hypothesis that chronic use of thickened liquid textures reduces bolus speed, reduces coordination of swallowing muscles, and disrupts normal respiratory-swallow event sequencing. Aim 2 will test the hypothesis that thickened liquids result in reduced volume of fluid intake and systemic dehydration, which will be exacerbated by viscosity and age. Aim 3 will test the hypothesis that thickened liquids, in the absence of thin liquid, will slow swallowing movements, dynamically altering muscle contractile properties to include reduced tongue forces and increased contraction times in an age-dependent manner. In concert, the proportion of fast- contracting type IIb myosin heavy chain (MyHC) isoforms in the tongue will be reduced, causing an increased proportion of slower-contracting isoforms, such as type IIa and type I. This research is highly significant in that it will be the first in an aging animal model to examine the effects of thickened liquids on systemic hydration along with underlying muscle biology, kinematics, and physiology, and the relationship to clinically relevant outcome measures of swallow function. Knowledge gained from this work will lead to hypotheses for testing in clinical studies that are crucial for clinical decisions that must balance patient risk of aspiration and subsequent medical complications, against the impact of potential deleterious effects of thickened liquids on patient overall medical status and quality of life.

抽象的 吞咽疾病在老年人中非常普遍，包括患有年龄相关的疾病的人。 增厚液体经常用于治疗吞咽障碍，并已证明 立即减少气道入侵的机会。但是，增稠的液体与 有害的健康影响，例如脱水，尿路感染和生活质量降低。尽管他们 常见的临床用途，增厚液体的长期功效以及它们对吞咽运动学的影响， 系统性水合和潜在的生物学尚不清楚。鉴于人口迅速增加 到2051年到2051年估计有65岁以上的人，了解吞咽困难的功效至关重要 干预措施。该提案的总体目标是为生物学贡献基础知识 功能性吞咽的基础机制，响应经常使用但研究过的响应， 老龄化人群中吞咽困难的干预。拟议的研究将使用老化的老鼠 询问使用加厚液体的使用模型如何影响吞咽运动学，全身水合和 随着时间的流逝，生物学的基础。我们有3个特定目标，每个目标都有一个独特的假设。 AIM 1将测试 假设长期使用增厚的液体质地会降低推注速度，从而降低 吞咽肌肉，并破坏正常的呼吸道戒断事件测序。 AIM 2将检验假设 增厚的液体导致液体摄入量和全身脱水的体积减少，这将是 因粘度和年龄而加剧。 AIM 3将检验以下液体增厚的假设 液体，会减慢吞咽运动，动态改变肌肉收缩特性以减少 舌作用和以年龄依赖的方式增加收缩时间。在协同的情况下，快速的比例 舌头中的IIB型肌球蛋白重链（MYHC）同工型将减少，从而增加 较慢的合同同工型（例如IIA型和I型）的比例。这项研究非常重要，因为 它将是老化动物模型中的第一个检查增厚液体对全身水合的影响 以及潜在的肌肉生物学，运动学和生理学以及与临床相关的关系 吞咽功能的结果度量。从这项工作中获得的知识将导致测试的假设 对于必须平衡患者抽吸风险和随后的临床决策至关重要的临床研究 医疗并发症，反对增稠液体对患者总体的潜在有害影响的影响 医疗状况和生活质量。