Integration of High Field MRI and EPRI For Functional Imaging

高场 MRI 和 EPRI 的集成用于功能成像

• 批准号: 9127730
• 负责人: JAY Louis ZWEIER
• 金额: $ 59.56万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2019-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9127730
• 关键词: Address; Algorithmic Software; Anatomy; Animal Disease Models; Animal Model; Animals; Back; Biochemical; Cardiac; Cardiovascular Physiology; Cardiovascular system; Cell Survival; Cell membrane; Cell physiology; Chemicals; Computer software; Consumption; Coupling; Data; Data Collection; Development; Disease; Electrons; Free Radicals; Frequencies; Functional Imaging; Generations; Health; Heart; Heart Diseases; Image; Imaging technology; Injury; Life; Magnetic Resonance; Magnetic Resonance Imaging; Maps; Measurement; Measures; Mediator of activation protein; Metabolism; Modality; Modeling; Motion; Myocardial; Myocardial Infarction; Nitric Oxide; Noise; Organ; Organ Model; Oxidation-Reduction; Oxygen; Oxygen saturation measurement; Pathology; Perfusion; Physiological; Physiology; Power Sources; Protons; Reactive Oxygen Species; Respiration; Role; Sampling; Scanning; Source; Spin Trapping; Stress; System; Techniques; Technology; Testing; Time; Tissue imaging; Tissues; Work; base; cardiovascular injury; cell injury; free radical oxygen; heart function; image processing; image registration; imaging modality; in vivo; innovation; instrument; instrumentation; prevent; programs; prototype; software development; support tools

DESCRIPTION (provided by applicant): Paramagnetic molecules including oxygen, oxygen radicals and nitric oxide modulate cellular function and injury, altering critical cellular processs such as respiration and redox state with changes in tissue perfusion, energetic state and viability. In view of the critical importance of these interactions in normal cardiovascular functin and disease, there has been a great need for an approach to combine in vivo measurement and imaging of paramagnetic molecules with imaging of tissue and organ biochemical and functional parameters in animal models of disease. EPR imaging (EPRI) is a powerful technique that enables 3D spatial in vivo mapping of radical metabolism, oxygenation, and nitric oxide; however, the utility and power of EPRI has been greatly limited by the need for complementary anatomic and functional data. NMR based MRI, in addition to providing anatomic registration, also can provide critical functional information that is needed to interpret the EPRI data including, imaging of tissue perfusion and viability. Integrated EPR/NMR imaging technology and instrumentation has the unique potential to enable in vivo mapping of free radicals, oxygen and nitric oxide along with NMR based functional and anatomic imaging. This technology is very promising but several fundamental problems presently limit its development and biomedical application. These include the need to: [a] provide seamless integration of the EPR and NMR-MRI systems to enable rapid transition between these two imaging modalities; [b] build dual mode resonators optimized for both EPR and NMR resonances suited for specific biomedical applications; [c] accelerate the process of image data collection in both modes facilitating dual mode anatomic and functional imaging; [d] increase sensitivity and minimize motion-induced noise or image distortion; [e] integrate the EPRI and MRI results and analysis in order to perform accurate image co-registration, fast computation and interpretation of functional data. These critical needs will be addressed with a combination of hardware and software development followed by testing in cardiovascular applications as described in 3 specific aims: I. Development and construction of EPRI hardware optimized for high sensitivity fast scan image acquisition and integration of it with high field MRI; II. Development / implementation of integrated software and algorithms for fast EPRI and NMR MRI acquisition with cardiac gating enabling co-mapping of anatomic structure, perfusion, energetic state and viability (MRI) with free radical distribution, redox state and oximetry (EPRI). EPRI acquisition will be accelerated and refined using NMR image information to refine EPR data collection; III. Application for dual mode functional imaging of the heart with both in vivo and ex vivo measurement of tissue O2 levels, redox status, nitric oxide formation (EPRI) in relation to perfusion, viability, and energeic state (MRI). This program will realize the great synergy and power of integrated real time EPRI and high field MRI optimized for biomedical measurement and imaging of free radicals, redox state, O2, and nitric oxide and their role in function and disease.

描述（由申请人提供）：包括氧、氧自由基和一氧化氮的顺磁性分子调节细胞功能和损伤，通过改变组织灌注、能量状态和活力来改变关键的细胞过程，例如呼吸和氧化还原状态。鉴于这些相互作用在正常心血管功能和疾病中的至关重要性，非常需要一种将顺磁性分子的体内测量和成像与疾病动物模型中的组织和器官生化和功能参数的成像相结合的方法。 EPR 成像 (EPRI) 是一种强大的技术，可实现自由基代谢、氧合作用和一氧化氮的 3D 体内空间绘图；然而，由于需要补充解剖和功能数据，EPRI 的实用性和威力受到极大限制。基于 NMR 的 MRI 除了提供解剖配准之外，还可以提供解释 EPRI 数据所需的关键功能信息，包括组织灌注和活力成像。集成 EPR/NMR 成像技术和仪器具有独特的潜力，可以实现自由基、氧和一氧化氮的体内绘图以及基于 NMR 的功能和解剖成像。这项技术非常有前途，但目前有几个基本问​​题限制了其发展和生物医学应用。其中包括需要： [a] 提供 EPR 和 NMR-MRI 系统的无缝集成，以实现这两种成像模式之间的快速转换； [b] 构建针对 EPR 和 NMR 谐振进行优化的双模式谐振器，适合特定的生物医学应用； [c] 加速两种模式下的图像数据收集过程，促进双模式解剖和功能成像； [d] 提高灵敏度并最大限度地减少运动引起的噪声或图像失真； [e] 整合 EPRI 和 MRI 结果及分析，以执行准确的图像共同配准、快速计算和功能数据的解释。这些关键需求将通过硬件和软件开发的结合来解决，然后在心血管应用中进行测试，如 3 个具体目标所述： I. 开发和构建针对高灵敏度快速扫描图像采集及其与高场集成而优化的 EPRI 硬件核磁共振成像；二.开发/实施集成软件和算法，用于快速 EPRI 和 NMR MRI 采集，通过心脏门控实现解剖结构、灌注、能量状态和活力 (MRI) 与自由基分布、氧化还原状态和血氧测定 (EPRI) 的联合映射。 EPRI 采集将使用 NMR 图像信息来加速和完善，以完善 EPR 数据收集；三．用于心脏双模式功能成像的应用，可体内和离体测量与灌注、活力和能量状态 (MRI) 相关的组织 O2 水平、氧化还原状态、一氧化氮形成 (EPRI)。该计划将实现集成实时 EPRI 和高场 MRI 的巨大协同作用和力量，优化用于自由基、氧化还原态、O2 和一氧化氮及其在功能和疾病中的作用的生物医学测量和成像。

项目成果

Supramolecular host-guest interaction of trityl-nitroxide biradicals with cyclodextrins: modulation of spin-spin interaction and redox sensitivity.

三苯甲基氮氧双自由基与环糊精的超分子主客体相互作用：自旋-自旋相互作用和氧化还原敏感性的调节。

• 发表时间: 2016-02-07
• 影响因子: 3.2
• 作者: Tan, Xiaoli;Song, Yuguang;Liu, Huiqiang;Zhong, Qinwen;Rockenbauer, Antal;Villamena, Frederick A;Zweier, Jay L;Liu, Yangping
• 通讯作者: Liu, Yangping

Mitochondrial Electron Transport Chain-Derived Superoxide Exits Macrophages: Implications for Mononuclear Cell-Mediated Pathophysiological Processes.

线粒体电子传递链衍生的超氧化物退出巨噬细胞：对单核细胞介导的病理生理过程的影响。

• 发表时间: 2016
• 作者: Li, Yunbo;Zhu, Hong;Kuppusamy, Periannan;Zweier, Jay L;Trush, Michael A
• 通讯作者: Trush, Michael A