Synaptic Organization of Simple Cell Receptive Fields

简单细胞感受野的突触组织

基本信息

批准号：
9332729
负责人：
Diego Contreras
金额：
$ 56.92万
依托单位：
UNIVERSITY OF PENNSYLVANIA
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-08-01 至 2022-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9332729
关键词：
Architecture Attention Autistic Disorder Automobile Driving Axon Behavior Cells Cerebral cortex Closure by clamp Data Electrophysiology (science)Environment Exhibits Felis catus Frequencies Image Individual Injection of therapeutic agent Knowledge Lateral Geniculate Body Light Measures Mental Depression Mental disorders Methods Minority Modeling Neurons Noise Operative Surgical Procedures Output Pattern Picrotoxin Play Property Rodent Role Schizophrenia Seminal Sensory Site Structure Synapses Techniques Testing Thalamic structure Time Variant Vision Visual Visual Acuity Visual Cortex Visual system structure area striata comparative experimental study extracellular gabazine in vivo insight millisecond novel strategies orientation selectivity postsynaptic receptive field response spatiotemporal synaptic function synaptic inhibition virtual visual information

项目摘要

PROJECT SUMMARY Image representation in primary visual cortex depends critically on the spatiotemporal pattern of convergence of lateral geniculate axons as well as on the dynamic properties of thalamocortical synapses. Thalamic input represents only a fraction of the excitatory drive to cortical cells in layer 4 ,but dominates their visual response patterns. We will use electrophysiological methods in vivo to record visual responses simultaneously from thalamorecipient neurons in primary visual cortex and several of their input cells in the lateral geniculate nucleus. In Aim 1, we will test the hypothesis that inhibition controls the temporal course of simple cells in layer 4. In Aim 2, we will characterize the properties of the postsynaptic potentials from single LGN neurons onto excitatory and inhibitory simple cells in layer 4. Finally, in Aim 3, we will use dynamic clamp in layer 4 simple cells to study convergent input from combinations of single LGN neurons (using knowledge obtained in Aims 1 and 2) without concomitant activation of cortical circuits. We will estimate the contributions of local inhibition in establishing L4 simple cell visual responses. These studies will generate critical insight into the transformation of visual information that takes place in the thalamocortical synapse.

项目概要初级视觉皮层的图像表征很大程度上取决于图像的时空模式外侧膝状体轴突的收敛以及丘脑皮质的动态特性突触。丘脑输入仅代表皮质细胞兴奋驱动的一小部分第 4 层，但主导他们的视觉反应模式。我们将使用电生理学方法体内同时记录初级丘脑受神经元的视觉反应视觉皮层及其在外侧膝状核中的几个输入细胞。在目标 1 中，我们将检验抑制控制第 4 层简单细胞的时间过程的假设。在 Aim 中 2，我们将表征单个 LGN 神经元的突触后电位的特性到第 4 层的兴奋性和抑制性简单细胞上。最后，在目标 3 中，我们将使用动态钳在第 4 层简单细胞中研究单个 LGN 神经元组合的收敛输入（使用目标 1 和 2 中获得的知识）无需同时激活皮质回路。我们将估计局部抑制对建立 L4 简单细胞视觉的贡献回应。这些研究将对视觉的转变产生批判性的见解丘脑皮质突触中发生的信息。