Protein-Trap in Vasculogenesis and Angiogenesis

血管生成和血管生成中的蛋白质陷阱

• 批准号: 7024357
• 负责人: THOMAS N SATO
• 金额: $ 10.87万
• 依托单位: WEILL MEDICAL COLL OF CORNELL UNIV
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7024357
• 关键词: angiogenesis; antibody; cell differentiation; cell proliferation; clone cells; developmental genetics; electroporation; embryonic stem cell; genetic library; genetically modified animals; laboratory mouse; posttranslational modifications; protein localization

DESCRIPTION (provided by applicant): My long-term goal is to understand molecular mechanisms of cell differentiation and development with particular emphasis on vasculogenesis and angiogenesis. Major effort has been made to discover genes that play critical roles in cell differentiation and development. Although this "gene hunting" effort has been paying off recently, there is major pit-fall in this type of approach. We have invented a new version of "protein-trap" approach, which enables us to discover proteins that escape the hunting effort made by any of the currently used gene-discovery methods. Furthermore, we expect to gain novel mechanistic insights into the processes of cell differentiation and development including vasculogenesis and angiogenesis based on the discovery made by this protein-type approach. This is a R21 application to request for the two year funding that enables us to use this novel approach to discover key proteins in cell differentiation and development. Initially, we will focus on proteins expressed during vasculogenesis and/or angiogenesis. However, our goal is not limited to these classes of proteins. We are also broadly interested in other classes of proteins that play key roles in cell differentiation and development in general. We expect that this two-year funding will enable us to make the initial discovery. This initial discovery is expected to form a basis for us to move on to more functional and mechanistic studies that could be funded through R01 mechanism following the completion of this project. We expect our discovery has immediately impact on the invention of novel and effective therapeutics targeted to many clinical areas including cancer, stem cell based regenerative medicine and cardiovascular diseases.

描述（由申请人提供）：我的长期目标是了解细胞分化和发育的分子机制，特别着重于血管生成和血管生成。已经做出了重大努力，以发现在细胞分化和发育中起关键作用的基因。尽管最近的“基因狩猎”努力已经获得了回报，但这种方法在这种方法中仍存在重大坑。我们发明了一种新版本的“蛋白质陷阱”方法，使我们能够发现蛋白质逃避任何当前使用的基因分辨方法所做的狩猎工作。此外，我们希望根据这种蛋白质型方法的发现，对细胞分化和发育过程（包括血管生成和血管生成），包括血管生成和血管生成。这是R21的应用程序，要求要求我们使用这种新颖的方法来发现细胞分化和开发中的关键蛋白质。最初，我们将专注于在血管生成和/或血管生成期间表达的蛋白质。但是，我们的目标不仅限于这些类别的蛋白质。我们也对其他在细胞分化和开发中起关键作用的蛋白质的其他类别感兴趣。我们预计这两年的资金将使我们能够最初的发现。预计这一最初的发现将为我们提供更多功能和机械研究的基础，这些研究可以通过R01机制资助，该研究可以通过R01机制资助。 我们预计我们的发现会立即影响针对许多临床领域的新型和有效治疗剂的发明，包括癌症，基于干细胞的再生医学和心血管疾病。