MOUSE MODELS FOR HUMAN CANCER

人类癌症小鼠模型

• 批准号: 6633571
• 负责人: WILLIAM Franklin DOVE
• 金额: $ 54万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-30 至 2004-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6633571
• 关键词: Hodgkin's disease; adenocarcinoma; animal genetic material tag; bioengineering /biomedical engineering; biomarker; cervix neoplasms; clinical research; cooperative study; disease /disorder model; fluorescent dye /probe; gene mutation; gene targeting; genetic models; genetically modified animals; human tissue; intestine neoplasm; laboratory mouse; magnetic resonance imaging; melanoma; model design /development; mutant; neoplasm /cancer genetics; pancreas neoplasms; retinoblastoma; technology /technique development

A group of experienced investigators in cancer genetics and human cancer virology at the University of Wisconsin have organized a set of projects that would synergize with the MMHCC. These projects include de novo derivations of mouse models for ductal pancreatic carcinoma, cervical carcinoma, uveal melanoma, and Hodgkin's Disease. Further, these investigators propose the further development of extant mouse models for intestinal neoplasia and retinoblastoma leading to validation against the corresponding human disease. More globally, it is proposed to broaden the range of mouse models displaying genetic instability, including deficiencies in double-strand break repair and G-T mismatch repair. These deficiencies will be studied within the set of tissue-specific mouse models for cancer, seeking acceleration of the pathogenetic process within the one-year lifespan of the mouse. Finally, two initiatives of technology development are proposed. One seeks high-resolution MRI imaging at 9.4 Tesla in order to follow directly the regression of mouse tumors whose sizes are often in the mm range. The second seeks a way to develop fluorigenic markers by which to characterize the distinct cell types of the normal intestinal epithelium, document the source of tumors under different genetic and environmental conditions, and cross-reference between mouse and human tumors.

威斯康星大学的一群经验丰富的癌症遗传学和人类癌症病毒学研究人员组织了一组将与MMHCC协同作用的项目。 这些项目包括针对导管胰腺癌，宫颈癌，紫外黑色素瘤和霍奇金氏病的小鼠模型的从头推导。 此外，这些研究者提出了用于肠道肿瘤和视网膜细胞瘤的现存小鼠模型的进一步发展，从而对相应的人类疾病进行了验证。 在全球范围内，建议扩大显示遗传不稳定性的小鼠模型的范围，包括双链断裂修复和G-T不匹配修复中的缺陷。 这些缺陷将在组织特异性小鼠模型的集合中进行研究，并在小鼠的一年寿命内寻求致病过程的加速。 最后，提出了两项​​技术开发计划。 一个人在9.4 Tesla处寻求高分辨率MRI成像，以直接跟随小鼠肿瘤的回归，其大小通常在MM范围内。 第二个寻求一种开发荧光标记的方法，通过该标记来表征正常肠上皮的不同细胞类型，记录在不同遗传和环境条件下肿瘤的来源，以及小鼠和人类肿瘤之间的交叉引用。