NANOTUBE-BASED ELECTRONIC BIOSENSORS

基于纳米管的电子生物传感器

• 批准号: 6690869
• 负责人: Gang Gu
• 金额: $ 13.7万
• 依托单位: MOLECULAR NANOSYSTEMS, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2005-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6690869
• 关键词: bioengineering /biomedical engineering; biomedical equipment development; biosensor device; carbon; computer program /software; electrodes; microarray technology; nanotechnology; robotics; semiconduction

DESCRIPTION (provided by applicant): The goal of the project is to develop a novel sensor technology platform based on carbon nanotube electronic sensor device, which could be integrated into a biochip and used for detection and analysis of biomolecules in samples from blood, saliva and other body fluids, as well as studies of protein-protein and protein-small molecule interactions in the research laboratory. The fundamental principle of the technology has been demonstrated by Professor Hongjie Dai's group at Stanford University, showing that the electrical resistance change of a nanotube can be detected when a biological event such as antigen-antibody binding occurs on the surface of the nanotubes. In the proposed electronic sensor array each nanotube sensor will be chemically functionalized and immobilized with biomolecules to provide selectivity and specificity for detection of various analytes. The detection scheme is based on changes in the nanotube's electrical conductance due to changes of the electrostatic environment upon analyte binding. Such detection system requires no expensive detection equipment, such as lasers, or fluorescence labeling of analytes. Instead the detection is based on a simple direct electrical readout. The objective of this program is to demonstrate the feasibility of a nanosensor providing the sensitivity and selectivity for detection of a biological marker, which is integrated into an electrical circuitry for easy readout. The specific aims include: (1) the fabrication of electrical devices containing semiconducting single-walled carbon nanotubes; this involves controlled growth of nanotubes on wafers by chemical vapor deposition and construction of electrodes and electrical circuits on wafers by semiconductor technology, (2) the functionalization of the nanotubes in the electrical devices for immobilization of biomolecules onto the nanotubes. A variety of functionalization strategies will be investigated to minimize non-specific adsorption and to maximize selectivity. Successful demonstration of the proposed nanotube-based biosensor will have a significant impact on a number of commercial sectors and help establish the United States as leader in the emerging field of nanotechnology.

描述（由申请人提供）：该项目的目的是基于碳纳米管电子传感器设备开发一种新型的传感器技术平台，该平台可以集成到生物芯片中，用于检测和分析血液，唾液和其他体液中的样品中的生物分子，以及蛋白质蛋白质和蛋白质 - 蛋白质 - 蛋白质 - 蛋白质 - 蛋白质互动的研究。洪吉·戴（Hongjie Dai）教授在斯坦福大学（Stanford University）的小组已经证明了该技术的基本原理，这表明当纳米管的表面发生抗原抗体结合（抗原抗体结合）时，可以检测到纳米管的电阻变化。在提出的电子传感器阵列中，每个纳米管传感器将化学功能化并用生物分子固定，以提供选择性和特异性，以检测各种分析物。检测方案基于纳米管电导的变化，这是由于分析物结合后静电环境的变化而导致的。这种检测系统不需要昂贵的检测设备，例如激光器或分析物的荧光标记。相反，检测基于简单的直接电气读数。该程序的目的是证明纳米传感器的可行性，从而提供了检测生物标记物的灵敏度和选择性，该生物标记物已集成到电路中以方便读数。具体目的包括：（1）制造含有半导体单壁碳纳米管的电气设备；这涉及通过化学蒸气沉积在晶片上控制纳米管的生长，并通过半导体技术对晶片的电极和电路的构建，（2）纳米管在电气设备中的官能化以将生物分子固定到纳米管上。将研究各种功能化策略，以最大程度地减少非特异性吸附并最大化选择性。拟议的基于纳米管的生物传感器的成功演示将对许多商业领域产生重大影响，并帮助将美国确立为纳米技术新兴领域的领导者。