Development of Bioelectric Field Imaging Instrumentation

生物电场成像仪器的研制

• 批准号: 6693938
• 负责人: RICHARD Lee NUCCITELLI
• 金额: $ 17.62万
• 依托单位: BIOELECTROMED CORPORATION
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2004-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6693938
• 关键词: bioengineering /biomedical engineering; biomedical automation; biomedical equipment development; clinical biomedical equipment; electric field; electrical measurement; electrical potential; electrophysiology; galvanic skin response; imaging /visualization /scanning; ionic strengths; laboratory mouse; noninvasive diagnosis; skin disorder; skin disorder diagnosis; wound; wound healing

DESCRIPTION (provided by applicant): We have known for over a century, that human skin drives ionic current out of regions where the integrity of the epidermis has been breached (DuBois-Reymond, 1843,Ann.Phys.U.Chem.58:1) DuBois-Reymond used one of the earliest galvanometers to measure 1 uA leaving wounds in his skin. This has been confirmed using modern techniques and we now know that this current is driven out of the wound by the transepithelial potential generated across the epidermis by the apical-basal transport of Na+. As this wound current traverses the epidermis it generates a local electric field that points towards the wound from all directions around it. While we know that wound currents exist, we do not know the local electric field strength that they generate as they move through the skin. It is this electric field that can influence wound healing by moving molecules by electrophoresis or attracting cells by galvanotaxis. Our goal is to measure this wound field in vivo. This field has been measured near guinea pig skin wounds but never in humans (Barker et al., 1982,Am.J.Physiol. 242:R358). Our company has been developing a new instrument called the Bioelectric Field Imager (BFI) that will be able to measure these lateral fields non-invasively. Just as the EKG can provide information about heart disease by measuring voltages between the limbs, the BFI technique provides information about skin disease. This will have many important applications to improving human health including the design of new electrical therapies for healing chronic wounds and possibly the early detection and diagnosis of skin diseases such as melanoma and psoriasis. Our Specific Aim is to adapt a prototype, BFI device to non-invasively measure the skin surface potential distribution in the region surrounding mammalian skin wounds. We will optimize vibration amplitude, distance between skin and probe, and signal averaging. We will introduce scanning capabilities that allow the two-dimensional mapping of the surface potential distribution around a skin wound. Finally we will determine the reproducibility of the device by laboratory bench testing on well-defined mammalian wounds. This instrument will allow us to compare the endogenous fields near normally healing wounds with those near chronic wounds in order to determine if there are differences that might be reduced by electrotherapy. This technique also has the potential for early detection and diagnosis of various skin diseases including melanoma and psoriasis.

描述（由申请人提供）： 我们已经知道一个多世纪以来，人类的皮肤将离子电流驱逐出被违反表皮的完整性的区域（Dubois-Reymond，1843年，Ann.phys.u.Chem.58：1）dubois-reymond使用了最早的省级仪表仪，以1 UA的伤口，使他的皮肤受伤。这已通过现代技术证实，我们现在知道，这种电流是由Na+的顶端基质转运在表皮上产生的跨月势驱动的。当该伤口电流横穿表皮时，它会产生一个局部电场，该电场指向其周围各个方向的伤口。尽管我们知道存在伤口电流，但我们不知道它们在皮肤中移动时会产生的当地电场强度。正是这种电场可以通过电泳移动分子或通过galvanotaxis吸引细胞来影响伤口愈合。我们的目标是在体内测量这一伤口场。该领域已在豚鼠皮肤伤口附近进行测量，但在人类中从未测量过（Barker等，1982，Am.J.Physiol。242：R358）。我们公司一直在开发一种称为生物电场成像仪（BFI）的新仪器，该工具将能够非侵入性地测量这些横向场。正如心电图通过测量四肢之间的电压提供有关心脏病的信息一样，BFI技术也提供了有关皮肤病的信息。这将在改善人类健康方面具有许多重要的应用，包括设计新的电气疗法来治愈慢性伤口，以及可能的早期发现和诊断皮肤病，例如黑色素瘤和牛皮癣。我们的具体目的是调整原型BFI设备，以非侵入性测量哺乳动物皮肤伤口周围区域的皮肤表面电位分布。我们将优化振动幅度，皮肤和探针之间的距离以及平均信号。我们将介绍扫描功能，以允许对皮肤伤口周围的表面电位分布进行二维映射。最后，我们将通过对明确定义的哺乳动物伤口进行实验室长凳测试来确定设备的可重复性。该仪器将使我们能够将正常愈合伤口附近的内源性场与附近的慢性伤口进行比较，以确定是否存在差异可能会减少电疗法。该技术还具有早期检测和诊断各种皮肤病（包括黑色素瘤和牛皮癣）的潜力。