CRYOPRESERVATION OF MURINE GERMPLASM

鼠种质的冷冻保存

• 批准号: 6544910
• 负责人: LARRY E MOBRAATEN
• 金额: $ 39.86万
• 依托单位: JACKSON LABORATORY
• 依托单位国家: 美国
• 财政年份: 1982
• 资助国家: 美国
• 起止时间: 1982-05-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6544910
• 关键词: artificial fertilization; cryopreservation; egg /ovum; embryo /fetus preservation; genetic strain; genetically modified animals; in vitro fertilization; laboratory mouse; sperm; tissue resource /registry

DESCRIPTION (provided by applicant): The number of mouse strains used in biomedical research is growing rapidly as a result of the technology that allows one to selectively alter mammalian genomes. To insure rapid and efficient advancement of biomedical research, it is vitally important that these new transgenic, targeted mutant, and chemically mutagenized mice, along with other equally valuable mouse strains, be protected against accidental loss. Cryopreservation of embryos or sperm offers an economical means of preserving scientifically important strains for future biomedical research. TJL has established the largest repository for frozen germplasm of genetically defined strains and has over 25 years of experience in cryopreservation. The objective of this project is to assure the continuity of this repository and the availability of mice from preserved germplasm to the scientific community. The Specific Aims of this application are to: 1) maintain the infrastructure of the continually growing TJL repository; 2) recover and distribute mice from frozen germplasm as needed for scientific research; 3) cryopreserve germplasm from scientifically valuable strains of mice that are not supported by other specific funding sources; 4) carry out research to develop, refine, and/or acquire new methods to improve the efficiency and productivity of the cryopreservation resource; and 5) interact and coordinate efforts with other resource centers.

描述（由申请人提供）：由于该技术允许人们选择性改变哺乳动物基因组的技术，生物医学研究中使用的小鼠菌株数量正在迅速增长。 为了确保生物医学研究的快速有效进步，至关重要的是，这些新的转基因，靶向突变体和化学诱变的小鼠以及其他同样有价值的小鼠菌株受到保护，以防止意外损失。 胚胎或精子的冷冻保存提供了一种经济的手段，可以为未来的生物医学研究保存科学重要的菌株。 TJL已建立了最大的遗传学菌株生殖种质的最大存储库，并拥有超过25年的冷冻保存经验。 该项目的目的是确保该存储库的连续性以及从保存的种质到科学界的小鼠的可用性。 该应用程序的具体目的是：1）维护不断增长的TJL存储库的基础架构； 2）根据需要从冷冻种质中恢复和分配小鼠； 3）从其他特定资金来源支持的科学有价值的小鼠菌株中的冷冻水平种质； 4）进行研究以开发，完善和/或获取新方法，以提高冷冻保存资源的效率和生产力； 5）与其他资源中心进行互动和协调。