CRPRC ROTAVIRUS STUDY

CRPRC 轮状病毒研究

• 批准号: 3742107
• 负责人: GEOFFREY Y AKITA
• 金额: --
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3742107
• 关键词: Macaca mulatta; Rotavirus; disease /disorder model; enzyme linked immunosorbent assay; feces analysis; gastrointestinal infection; polymerase chain reaction

In an effort to develop a monkey model of rotavirus (RV) infection we have initially characterized natural RV infection in colony reared rhesus monkeys. Feces and serum were collected from 37 diarrheic monkeys of ages ranging from 8 to 61 months. Feces were screened by a PCR assay for group anti-RV and a commercial enzyme immunozassay (EIA). Virus isolation (VI) was performed on feces that were positive by either assay. Anti-RV IGG was quantitated in serum and fecal washes by elisa. RV antigen recognition by serum IGG was determined by immunoblot. Suitable reagents were not available for detection of anti-RV IGA. PCR was more sensitive in detecting fecal RV shedding than EIA. A RV was isolated from one PCR and EIA positive fecal sample. The majority of animals with sera available for testing had IGG titers to RV. The fecal RV positive animals were either serum antibody negative or sera was not available. Generally sera with higher elisa titers recognized more RV viral proteins (VP) by immunoblot. Anti-RV copro-IGG is present in the majority of animals assayed to date. Two animals with anti-RV copro-IGG were fecal RV positive. Natural RV infection in colony reared rhesus monkeys appears similar to natural RV infection in humans.

为了开发轮状病毒（RV）感染的猴子模型 最初表征了殖民饲养的恒河猴的天然RV感染 猴子。 粪便和血清是从37只腹泻猴子收集的 年龄从8到61个月不等。 通过PCR测定法对粪便进行筛选 组抗RV和商业酶免疫综合（EIA）。 病毒 分离（VI）是对通过两种测定的阳性的粪便进行的。 ELISA在血清和粪便洗涤中定量抗RV IgG。 RV 通过免疫印迹确定血清IgG抗原识别。 合适的 试剂无法检测抗RV IgA。 PCR更多 比EIA敏感检测粪便RV脱落。 RV隔离 来自一个PCR和EIA阳性粪便样品。 大多数动物 可用于测试的血清具有IgG滴度为RV。 粪便阳性 动物要么是血清抗体阴性的，要么是血清不可用的。 通常具有较高ELISA滴度的血清识别出更多的RV病毒蛋白 （VP）通过免疫印迹。 抗RV Copro-iGG大多数 迄今为止，动物分析了。 两只具有抗RV Copro-igg的动物是粪便 RV阳性。 殖民地饲养的恒河猴的天然RV感染 似乎类似于人类的天然RV感染。