SIV AND MACAQUE MODEL OF CHRONIC CRYPTOSPORIDIOSIS

慢性隐孢子虫病的 SIV 和猕猴模型

• 批准号: 3742080
• 负责人: NICHOLAS W LERCHE
• 金额: --
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3742080
• 关键词: Cryptosporidium; Macaca mulatta; cryptosporidiosis; disease /disorder model; model design /development; simian AIDSs; simian immunodeficiency virus

Chronic cryptosporidiosis is a common opportunistic infection in humans with AIDS and also in macaques with SIV-induced immunodeficiency. Cryptosporidiosis in SIV infected macaques is being evaluated as an animal model system for studying pathogenesis of cryptosporidium in immune compromised hosts, and for testing the efficacy of therapeutic interventions. Ten SIV-infected rhesus macaques, released from other SIV research projects have been enrolled in the study. Baseline data on clinical and immunologic status have been collected for "staging" of SIV-related disease. To date, four animals with CD4 cell counts above 250/ul and free of naturally acquired cryptosporidiosis have been inoculated orally with infectious oocysts, two with a dose of 100 oocysts and two with 1000 oocysts. Intermittent, low level shedding of oocysts has been observed in all 4 animals without diarrhea these animals showed no "boost" in serum IGG or in vitro lymphocyte proliferation against oocysts antigens. Analyses of saliva and gut lavage samples for cryptosporidium-specific IGA responses are in progress. Techniques have been established to harvest gut mucosal lymphocytes for immunologic analyses. In addition, two animals have been identified with naturally acquired cryptosporidium infection that are chronic shedders of oocysts, without clinical disease. Analysis of the cryptosporidium-specific immune responses of these animals is in progress.

慢性隐孢子虫病是人类常见的机会感染 具有辅助物以及具有SIV诱导的免疫缺陷的猕猴。 SIV感染的猕猴中的隐孢子虫病被评估为一种 用于研究隐孢子虫发病机理的动物模型系统 免疫受损的宿主，并用于测试治疗的功效 干预措施。 从其他SIV释放的十个SIV感染的恒河猕猴 研究项目已入学。 基线数据 为“分期”收集了临床和免疫状态 与SIV相关的疾病。 迄今为止，四只具有CD4细胞计数的动物 250/UL，没有自然获得的隐孢子虫病 用传染性卵囊接种口服，两个剂量为100卵囊 和两个卵囊。 间歇性低水平的卵囊 这些动物在没有腹泻的所有四只动物中都观察到 在血清IgG或体外淋巴细胞增殖中无“增强” 卵囊抗原。 唾液和肠道灌洗样品的分析 隐孢子虫特异性IgA响应正在进行中。 技术有 建立以收获肠粘膜淋巴细胞的免疫学 分析。 另外，已经自然地发现了两只动物 获得的隐孢子虫感染是卵囊的慢性棚架， 没有临床疾病。 分析隐孢子虫特异性 这些动物的免疫反应正在进行中。