ENHANCED DIAGNOSTICS FOR SIMIAN TYPE D RETROVIRUS

改进的猴 D 型逆转录病毒诊断

• 批准号: 3742078
• 负责人: NICHOLAS W LERCHE
• 金额: --
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3742078
• 关键词: Macaca; communicable disease diagnosis; diagnosis design /evaluation; polymerase chain reaction; type D retrovirus group

Eleven macaques (4 rhesus, 1 pig-tail, and 6 long-tail) found to be naturally infected with various serotypes of Simian type D retrovirus (SRV/D) during routine colony and quarantine screening, will be maintained in BSL-2 housing to provide blood and other biological samples for use in the development of enhanced diagnostic techniques, including polymerase chain reaction (PCR) for the detection of integrated proviral DNA; reverse transcriptase (RT)- PCR for the detection of viral RNA in plasma and other body fluids; and synthetic peptide based enzyme immunoassays for detection of SRV/D-specific antibodies. To date, gag and env PCR primer pairs have been developed that are capable of recognizing SRV serotypes 1,2,3 in infected cell cultures, peripheral blood and lymphoid tissues. Development of pol gene primers able to recognize all 5 known SRV serotypes is currently in progress.

11 只猕猴（4 只恒河猴、1 只猪尾猴和 6 只长尾猴）被发现 自然感染猿 D 型逆转录病毒的各种血清型 (SRV/D) 在例行菌落和检疫筛查期间，将 保存在 BSL-2 外壳中以提供血液和其他生物样本 用于开发增强诊断技术，包括 用于检测整合原病毒的聚合酶链反应 (PCR) 脱氧核糖核酸;逆转录酶 (RT) - PCR 用于检测病毒 RNA 血浆和其他体液；和合成肽基酶 用于检测 SRV/D 特异性抗体的免疫测定。 迄今为止，堵嘴 和 env PCR 引物对已经开发出来，能够 识别受感染细胞培养物、外周血中的 SRV 血清型 1、2、3 血液和淋巴组织。 pol 基因引物的开发能够 目前正在进行识别所有 5 种已知 SRV 血清型的工作。