A platform for innovation in miniature microscopy

微型显微镜创新平台

• 批准号: 9193420
• 负责人: Timothy James Gardner
• 金额: $ 27.93万
• 依托单位: BOSTON UNIVERSITY (CHARLES RIVER CAMPUS)
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-01 至 2020-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9193420
• 关键词: 3D Print; Address; Adolescent; Adopted; Animals; Behavior; Boston; Brain; Cell Nucleus; Collaborations; Color; Communities; Computer software; Devices; Disease model; Documentation; Electrophysiology (science); Event; Feedback; Fluorescence; Goals; Head; Housing; Image; Internet; Laboratories; Learning; Letters; Methods; Microscope; Microscopy; Modification; Mus; Neurons; Neurosciences; Pattern; Population; Process; Research; Resolution; Resources; Services; Site; Site-Directed Mutagenesis; Songbirds; Source; Source Code; Structure; Surface; System; Telemetry; Testing; Time; Training; Universities; Variant; Video Recording; Weight; Wireless Technology; awake; base; calcium indicator; cell type; cost; design; flexibility; fluorescence microscope; image processing; innovation; invention; meetings; novel; online repository; open source; optical imaging; relating to nervous system; repository; research study; response; sensor; tool; web-accessible; wiki

Miniature head-mounted fluorescence microscopes allow neuroscientists to record from populations of neurons longitudinally at cellular resolution in freely moving animals. However, off-the-shelf devices currently lack a number of desirable features such as easy modification, wireless interfacing, and flexible real-time analysis software. This project will disseminate an open-source miniature microscope (“miniscope”) that meets these needs. New features realized in the miniscope include a 3D-printed housing for easy microscope reconfiguration, wireless telemetry, and color CMOS sensors for simultaneous recording of multiple fluorescence indicators. In addition to the microscope, this project disseminates open-source software for controlling the microscope. This software is capable of real-time image processing and feedback for closed-loop experiments that trigger stimulation or other events in response to patterns of recorded neural activity. The strategy for dissemination is two-fold. First, the project provides fully functioning miniscopes and associated components and training for 14 collaborating labs. These end-users will use the microscopes to address a wide range of questions in surface and deep brain nuclei, focused on many distinct cell types. These groups will study learning in normal brain functioning and pathological activity patterns in multiple disease models. Second, the project will create a public web repository containing resources and documentation necessary to reproduce the microscope in other laboratories. Finally, the project will implement a number of design variations requested by end-users. These design variations include changes in the field of view, specialized microscope housings, adaptations for simultaneous electrophysiology, new color imaging strategies, and user-defined changes to the real-time software. These design variations will also be described in the public web resources. Ultimately, the goal of the project is to provide a platform for innovation in the use of customized miniature microscopes.

微型头戴式荧光显微镜使神经科学家能够以细胞分辨率纵向记录自由活动动物的神经元群，但是，现成的设备目前缺乏许多理想的功能，例如易于修改、无线接口和灵活的实时功能。该项目将推广满足这些需求的开源微型显微镜（“微型显微镜”）。微型显微镜中实现的新功能包括可轻松重新配置显微镜的 3D 打印外壳、无线遥测和颜色。用于同时记录多个荧光指示器的 CMOS 传感器 除了显微镜之外，该项目还传播用于控制显微镜的开源软件，该软件能够对触发刺激或其他的闭环实验进行实时图像处理和反馈。传播策略有两个方面：首先，该项目为 14 个合作实验室提供功能齐全的微型显微镜和相关组件以及培训。的其次，该项目将创建一个公共网络存储库，其中包含复制所需的资源和文档。最后，该项目将实现最终用户要求的许多设计变化，这些设计变化包括视野的变化、专用显微镜外壳、同步电生理学的适应、新的彩色成像策略和用户。 - 实时定义的变化这些设计变化也将在公共网络资源中进行描述，该项目的最终目标是为定制微型显微镜的使用提供一个创新平台。