N6-methyladenosine (m6A) Interplays with RNA and DNA Damage to Regulate DNA Repair

N6-甲基腺苷 (m6A) 与 RNA 和 DNA 损伤相互作用以调节 DNA 修复

• 批准号: 10835455
• 负责人: Yuan Liu
• 金额: $ 2.43万
• 依托单位: FLORIDA INTERNATIONAL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-24 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10835455
• 关键词: Biological Assay; DNA Damage; DNA Modification Process; DNA Polymerase beta; DNA Repair; Deposition; Equipment; Funding; Genes; Goals; Grant; Laboratories; Maps; Mediating; Modeling; National Institute of Environmental Health Sciences; OGG1 gene; RNA; Request for Applications; Research; Services; Time; Transcript; Validation; cancer cell; knock-down; knockout gene; repaired; success; Biological Assay; DNA Damage; DNA Modification Process; DNA Polymerase beta; DNA Repair; Deposition; Equipment; Funding; Genes; Goals; Grant; Laboratories; Maps; Mediating; Modeling; National Institute of Environmental Health Sciences; OGG1 gene; RNA; Request for Applications; Research; Services; Time; Transcript; Validation; cancer cell; knock-down; knockout gene; repaired; success

Project Summary The equipment supplement application requests funds to purchase a Bio-Rad real-time PCR machine that is essential for the validation of OGG1 gene knockout and METTL3 gene knockdown and for mapping the profiles of m6A and RNA and DNA damage on the DNA polymerase β (Pol β) gene in cancer cells under the aims of the PI’s NIEHS active grant R03ES0350200. The strand break-mediated RNA and DNA modification profiling assays (SBRM and SBRM) used for mapping m6A and RNA and DNA damage on the Pol β gene are PCR-based and critical for the success of the proposed studies. The old real-time PCR machine in the PI’s laboratory was broken recently. It cannot be repaired because the old model and its repair service were discontinued by Bio-Rad Laboratories. A new real- time PCR machine will allow us to accomplish the goals of the proposed studies on understanding the effects of RNA and DNA damage on m6A deposition on the Pol β gene and how m6A profiling changes can alter DNA repair by modulating RNA damage on the Pol β transcript in cancer cells. Thus, there is an urgent need to purchase the new real-time PCR machine that is essential for us to accomplish the research goals of our project on time.

项目摘要 设备补充申请要求资金购买Bio-Rad实时PCR机器 这对于验证OGG1基因敲除和Mettl3基因敲低以及 在DNA聚合酶β（polβ）基因中绘制M6A和RNA损伤的谱图 PI的NIEHS主动授予R03ES0350200的癌细胞。链断裂介导的 RNA和DNA修饰分析测定法（SBRM和SBRM）用于映射M6A和RNA，并且 polβ基因的DNA损伤基于PCR，对于拟议研究的成功至关重要。 PI实验室中的旧实时PCR机器最近被打破了。它无法修复 因为旧模型及其维修服务是由Bio-Rad实验室停止的。一个新的真实 时间PCR机器将使我们能够实现有关理解的拟议研究的目标 RNA和DNA损伤对M6A沉积对POLβ基因的影响以及M6A分析如何变化 可以通过调节癌细胞中POLβ转录物对RNA损伤的RNA损伤来改变DNA修复。那是 迫切需要购买新的实时PCR机器，这对于我们完成 我们项目的研究目标准时。