A multi-laboratory validation of the extraction of fat-soluble vitamins from tissue and feed and their analysis by LC-MS/MS

从组织和饲料中提取脂溶性维生素及其 LC-MS/MS 分析的多实验室验证

• 批准号: 10829100
• 负责人: John Philip Buchweitz
• 金额: $ 5.79万
• 依托单位: MICHIGAN STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10829100
• 关键词: multi; laboratory; validation; extraction; fat

PAR-18-604 Vet-LIRN Network Capacity-Building Project A multi-laboratory validation of the extraction of fat-soluble vitamins from tissue and feed and their analysis by LC-MS/MS Abstract The fat-soluble vitamins A, E, and D are essential dietary components for all animal species. Although required in small quantities, these compounds exert a multitude of metabolic and physiologic effects through all stages of an animal’s life. Because these vitamins or their precursors are naturally occurring, most free ranging animals can maintain adequate levels through the browse or forage they consume. However, processed grains and other ingredients used in the generation of commercial feeds for companion, production, and specialty species are challenged with the propensity of vitamins toward degradation. Accordingly, the commercial feed industry supplements diets with synthetic forms of these vitamins to provide adequate nutrient value. However, storage conditions and mixing errors can adversely impact the final concentration that is consumed. As a result, nutrient vitamin concentrations may be either inadequate and lead to clinical signs of deficiency or excessive and exhibit manifestations of toxicosis. Clinically, both deficiencies and toxicoses can be established ante-mortem through the measurement of serum vitamin A, E, or the vitamin D metabolite, 25-hydroxy vitamin D (25-OH D). For the post-mortem animal, tissue vitamin analysis is generally performed with liver being the sample of choice for vitamins A and E, and kidney being the best sample for 25-OH D. A clinical diagnosis that is suggestive of vitamin deficiency or excess generally (but not always) implicates a potential problem with feed. Current methods to address fat soluble vitamin content in feed are somewhat onerous, time-consuming, and may utilize outdated equipment. We propose a method of extraction and analysis for both tissues and feed that is simple, accurate, and reproducible across laboratories within the CVM Vet-LIRN community, Michigan State University Veterinary Diagnostic Laboratory (MSU VDL), Kansas State University Veterinary Diagnostic Laboratory (KSU VDL), Tennessee Department of Agriculture Kord Animal Health Diagnostic Laboratory (TN Ag Lab), Texas A&M University Veterinary Medical Diagnostic Laboratory (TVMDL), University of California Davis California Animal Health and Food Safety (CAHFS) lab, and University of Guelph Animal Health Laboratory (Guelph AHL) equipped with liquid chromatography tandem mass spectrometry.

PAR-18-604 VET LIRN网络能力建设项目 对从组织和饲料中提取脂溶性维生素及其的多样性验证 通过LC-MS/MS分析 抽象的 脂溶性维生素A，E和D是所有动物物种的重要饮食成分。尽管需要 这些化合物在少量的情况下在各个阶段发挥了多种代谢和生理作用 动物的生活。因为这些维生素或其前体是自然存在的，所以最自由的 动物可以通过消耗的浏览或饲料维持足够的水平。但是，加工的谷物 以及用于合作伙伴，生产和专业的商业用品中使用的其他成分 物种受到维生素降解的承诺的挑战。根据商业提要 用这些维生素的合成形式补充饮食，以提供足够的营养价值。然而， 存储条件和混合错误可能会对消耗的最终浓度产生不利影响。作为 结果，营养维生素浓度可能不足，并导致缺乏症或 过度和表现出毒理的表现。在临床上，可以建立缺陷和毒性 通过测量血清维生素A，E或维生素D代谢产物，25-羟基维生素的测量。 D（25-OH D）。对于死后动物，通常进行组织维生素分析，肝脏是 维生素A和E的首选样本，肾脏是25-OH D的最佳样本。 暗示维生素缺乏症或过量（但并非总是如此） 喂养。当前解决饲料中脂肪固体维生素含量的方法有些繁重，耗时， 并可能利用过时的设备。我们提出了一种针对组织和饲料的提取和分析方法 密歇根州CVM兽医社区内的实验室中，这是简单，准确且可再现的 州立大学兽医诊断实验室（MSU VDL），堪萨斯州立大学兽医诊断 田纳西州农业科德动物健康诊断实验室（TN）的实验室（KSU VDL） AG LAB），得克萨斯州农工大学兽医医学诊断实验室（TVMDL），加利福尼亚大学 戴维斯加州动物健康与食品安全（CAHFS）实验室和圭尔夫大学动物健康 实验室（Guelph AHL）配备了液相色谱串联质谱法。