Cellular and Molecular Biology Core

细胞和分子生物学核心

基本信息

批准号：
10704037
负责人：
Vinay Aakalu
金额：
$ 18.37万
依托单位：
UNIVERSITY OF ILLINOIS AT CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-09-01 至 2026-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10704037
关键词：
Cell Culture Techniques Cell Line Cells Cellular biology Cloning Core Grant Cultured Cells DNA DNA sequencing Equipment Flow Cytometry Goals Histologic Histology Individual MicroRNAs Molecular Biology Morphology Organ Culture Techniques Protein Analysis Proteomics Research Research Personnel Services Specimen Time Tissues Transfection Viral Packaging Viral Vector Vision research cost design experimental study instrument instrumentation laser capture microdissection skills stem cells virology

Cell Culture Techniques Cell Line Cells Cellular biology Cloning Core Grant Cultured Cells DNA DNA sequencing Equipment Flow Cytometry Goals Histologic Histology Individual MicroRNAs Molecular Biology Morphology Organ Culture Techniques Protein Analysis Proteomics Research Research Personnel Services Specimen Time Tissues Transfection Viral Packaging Viral Vector Vision research cost design experimental study instrument instrumentation laser capture microdissection skills stem cells virology

展开

项目摘要

PROJECT SUMMARY: CELLULAR AND MOLECULAR BIOLOGY CORE The Cellular and Molecular Biology Core provides essential equipment and support for molecular biology, cell culture, histology, and virology. Importantly, the Core contains a number of specialized instruments that are either too costly for individual investigators, necessary but infrequently used, or require skilled assistance to use. The goals are to provide facilities, expertise, and services for: 1) establishing, expanding, and maintaining primary cells, stem cells, and transformed, transfected, and inducible cell lines as well as organ cultures for use in vision research, 2) performing cellular and molecular biology experiments for ophthalmic research that require use of specialized instrumentation, 3) assisting and coordinating experiments that involve cell culturing, transfection, viral packaging, primer design, cloning, construction of mammalian and viral vectors, flow cytometry, laser capture microdissection, DNA sequencing, real time PCR, DNA/microRNA microarray, Luminex, proteomics, and protein analyses, and 4) assisting in histologic and morphological studies of tissue specimens and cultured cells.

项目摘要：细胞和分子生物学核心细胞和分子生物学核心为分子生物学提供必不可少的设备和支持文化，组织学和病毒学。重要的是，核心包含许多专业仪器对于个人调查人员来说，要么太昂贵了，因此需要使用，但很少使用，或者需要熟练的帮助才能使用。目标是为：1）建立，扩展和维护的设施，专业知识和服务原代细胞，干细胞和转化，转染和诱导细胞系以及器官培养物的在视觉研究中使用2）进行眼科研究的细胞和分子生物学实验需要使用专门的仪器，3）协助和协调涉及细胞培养的实验，转染，病毒包装，底漆设计，克隆，哺乳动物和病毒载体的构造，流量细胞仪，激光捕获微分解，DNA测序，实时PCR，DNA/microRNA微阵列， Luminex，蛋白质组学和蛋白质分析，以及4）有助于组织学和形态学研究标本和培养细胞。