Biospecimen Repository and Pathology Core

生物样本库和病理学核心

• 批准号: 10704083
• 负责人: GEORGE PLITAS
• 金额: $ 33.26万
• 依托单位: SLOAN-KETTERING INST CAN RESEARCH
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-13 至 2025-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10704083
• 关键词: Aftercare; Animal Model; Archives; Artificial Intelligence; Biological Assay; Biological Specimen Banks; Blood; Breast; Breast Cancer Model; Breast Cancer Patient; Chromosomal Instability; Clinical; Collection; Communities; Comparative Pathology; DNA Repair; Dedications; Defect; Development; Diagnosis; Drug or chemical Tissue Distribution; Ensure; Evaluation; Formalin; Freezing; Generations; Genetic; Genetically Engineered Mouse; Genomic Instability; Genomic approach; Guidelines; Histopathology; Human; Immunofluorescence Immunologic; Immunohistochemistry; Immunooncology; Immunophenotyping; Individual; Infrastructure; Institution; Institutional Review Boards; Laboratories; Memorial Sloan-Kettering Cancer Center; Metastatic breast cancer; Microdissection; Mus; Normal tissue morphology; Nucleic Acids; Paraffin Embedding; Pathologic; Pathologist; Pathology; Patients; Pattern; Phenotype; Pilot Projects; Preparation; Protocols documentation; Research; Research Personnel; Research Project Grants; Resources; Sampling; Scientist; Services; Standardization; System; Tissue Embedding; Tissue Microarray; Tissue Procurements; Tissue Sample; Tissues; Translational Research; biobank; cell free DNA; central database; comparative; data mining; design; digital; digital imaging; experience; imaging platform; immune cell infiltrate; malignant breast neoplasm; molecular pathology; mouse model; multiplexed imaging; neoplastic cell; novel; participant enrollment; patient derived xenograft model; peripheral blood; repository; research study; tissue processing; tumor; tumor microenvironment

PROJECT SUMMARY/ABSTRACT The Biospecimen Repository and Pathology Core is designed to provide support for the translational research efforts of the Genomic Instability in Breast Cancer SPORE, serving the SPORE RP1, RP2 and RP3, and developmental research projects. This Core will ensure a seamless integration of the current infrastructure available at Memorial Sloan Kettering Cancer Center (MSKCC), and constitute an essential component of this SPORE project, facilitating and expediting the collection, annotation, storage, distribution, and digital archiving and processing of biospecimens from breast cancer patients with specific patterns of DNA repair defects and/or genetic instability included in the SPORE research projects. This Core was also conceived to provide SPORE investigators with expert histopathologic evaluation of tumor samples from breast cancer patients enrolled in the research protocols, patient-derived xenografts and murine models described in this SPORE application, in conjunction with comparative pathologists from the MSKCC Laboratory of Comparative Pathology and the Genetically Engineered Mouse Phenotyping Service. The Core will also provide assistance in performing and interpreting immunohistochemical assays, in selecting tissue for microdissection and performing these microdissections, and in the generation of dedicated tissue microarrays. The Core will provide multiplexed immunophenotyping of the tumor and tumor microenvironment from breast cancers and breast cancer models. The specific aims of the Biospecimen Repository and Pathology Core include 1) to maintain and expand the systematic collection, annotation, and storage of bio-specimens for translational research of breast cancers with specific patterns of DNA repair defects and/or genomic instability, 2) to perform expert pathologic evaluation of all human breast cancer and animal models of breast cancer samples with specific patterns of DNA repair defects and/or genomic instability and preparation of appropriate material for use by SPORE investigators, and 3) to perform detailed immunophenotypic characterization of breast cancers and animal models of breast cancers with specific patterns of DNA repair defects and/or genomic instability. By centralizing and standardizing the pathology review of both human and murine breast cancer samples, from both clinical and research settings, and by working in conjunction with other Cores within MSKCC on the processing and curation of the biospecimen materials and digital images obtained from the samples included, the Biospecimen Repository and Pathology Core will help mitigate the impact of common confounders in translational research studies, as well as it will assist in the integration and prioritization of a variety of institutional pathology systems-related development efforts.

项目摘要/摘要 生物循环库和病理核心旨在为翻译研究提供支持 乳腺癌孢子中基因组不稳定性的努力，为孢子RP1，RP2和RP3服务以及 发展研究项目。该核心将确保当前基础架构的无缝集成 可在纪念斯隆·凯特林癌症中心（MSKCC）上找到，构成了此的重要组成部分 孢子项目，促进和加快收集，注释，存储，分发和数字归档 以及来自具有DNA修复缺陷和/或的特定模式的乳腺癌患者的生物测量 孢子研究项目中包括遗传不稳定。该核心也被认为提供孢子 对参加乳腺癌患者的肿瘤样本的专家组织病理学评估的研究者 研究方案，患者衍生的异种移植物和此孢子应用中描述的鼠模型， 与比较病理学的MSKCC实验室的比较病理学家的结合 基因工程的鼠标表型服务。核心还将在执行和 解释免疫组织化学测定，在选择微调的组织和执行这些组织时 微作用，以及专用组织微阵列的产生。核心将提供多重 乳腺癌和乳腺癌模型的肿瘤和肿瘤微环境的免疫表型。 生物测量存储库和病理核心的具体目的包括1）维护和扩展 生物特异性的系统收集，注释和存储，用于乳腺癌的翻译研究 DNA修复缺陷和/或基因组不稳定性的特定模式，2）执行专家病理学评估 所有人类乳腺癌和乳腺癌样品的动物模型，具有特定的DNA修复缺陷 和/或基因组不稳定性以及为孢子研究者使用适当的材料，3） 对乳腺癌和乳腺癌动物模型进行详细的免疫表型表征 具有DNA修复缺陷和/或基因组不稳定性的特定模式。通过集中和标准化 临床和研究环境中的人和鼠类乳腺癌样本的病理评论， 并通过与MSKCC中的其他核心合作进行生物测试的处理和策划 从包含的样品，生物测量存储库和病理学获得的材料和数字图像 核心将有助于减轻普通混杂因素在翻译研究中的影响，并将 协助整合和优先级 努力。