Core B - Large Animal Core

核心B - 大型动物核心

基本信息

批准号：
10677723
负责人：
GREGORY P WALCOTT
金额：
$ 48.89万
依托单位：
UNIVERSITY OF ALABAMA AT BIRMINGHAM
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-09-01 至 2027-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10677723
关键词：
Acceleration Adult Alabama Animal Model Animals Archives Arrhythmia Bioinformatics Cardiac Cardiac Myocytes Cell Cycle Complex Data Data Analyses Documentation Echocardiography Electric Stimulation Electrophysiology (science)Ensure Equipment Evaluation Family suidae Gene Delivery Housing Human Engineering Human Resources Implant Individual Injury Investigational Therapies Laboratories Leadership Magnetic Resonance Imaging Mammals Maps Mediating Methods Modeling Molecular Analysis Monitor Muscle Myocardial Myocardial tissue Neonatal Operative Surgical Procedures Optics Perioperative Care Predisposition Procedures Process Proliferating Protocols documentation Regulator Genes Reporting Reproducibility Research Personnel Resources Sampling Secure Site Structure Surgeon Tissues Training Transplantation Universities Ventricular Veterinarians animal resource biological preparation cost data quality experience experimental study good laboratory practice heart function heart rhythm hemodynamics histological specimens in vivo myocardial injury operation programs protocol development skills treatment effect

Acceleration Adult Alabama Animal Model Animals Archives Arrhythmia Bioinformatics Cardiac Cardiac Myocytes Cell Cycle Complex Data Data Analyses Documentation Echocardiography Electric Stimulation Electrophysiology (science)Ensure Equipment Evaluation Family suidae Gene Delivery Housing Human Engineering Human Resources Implant Individual Injury Investigational Therapies Laboratories Leadership Magnetic Resonance Imaging Mammals Maps Mediating Methods Modeling Molecular Analysis Monitor Muscle Myocardial Myocardial tissue Neonatal Operative Surgical Procedures Optics Perioperative Care Predisposition Procedures Process Proliferating Protocols documentation Regulator Genes Reporting Reproducibility Research Personnel Resources Sampling Secure Site Structure Surgeon Tissues Training Transplantation Universities Ventricular Veterinarians animal resource biological preparation cost data quality experience experimental study good laboratory practice heart function heart rhythm hemodynamics histological specimens in vivo myocardial injury operation programs protocol development skills treatment effect

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项目摘要

ABSTRACT (Core B) Large Animal Model and Electrophysiology Core Experiments in large mammals are a defining feature of this P01. All three projects require analyses of cardiac function in neonatal or adult pigs, but the housing, surgical, and analytical requirements are too expensive to be conducted in an individual laboratory and typically require expertise in the use of specialized and sophisticated equipment. Thus, the Large Animal Core (Core B) will provide a centralized facility for all surgical procedures (e.g., induction of myocardial injury, treatment administration, perioperative care), cardiac functional assessments (magnetic resonance imaging [MRI], hemodynamics), and electrophysiological evaluations (programmed electrical stimulation, implanted loop recorders, optical mapping). Core B will be located at the University of Alabama at Birmingham in the Cardiac Rhythm Management Laboratory (CRML), and animals will be housed at the adjacent Animal Resource Program (ARP). Collectively, these two facilities have all of the resources and equipment needed to conduct our proposed experiments and are staffed by an exceptionally skilled, efficient, and experienced team of investigators, cardiac surgeons, veterinarians, and technicians. The responsibilities of Core B are organized under the following three Specific Aims (SAs): SA 1, Induction of myocardial injury in neonatal and adult pigs; SA 2, Delivery of experimental therapy (i.e., transplantation of engineered human myocardial tissue patches [hMTPs] or state-of-the-art [AAV- or modRNA-mediated] gene delivery); and SA 3, Electrophysiological and functional assessments. Core B will also collect tissues, prepare biological samples for histological and molecular analysis, coordinate the shipment of tissues and samples to all Projects, and ensure that all experimental data, protocols, reports, and other documentation are securely stored, archived, and shared among Projects and Cores. Notably, all experimental procedures will be rigorously and consistently defined and executed for all Projects, which will accelerate the pace of scientific discovery by enabling us to draw valid conclusions from comparisons across the full range of experiments conducted under this PPG.

抽象的（核心B）大动物模型和电生理核心大型哺乳动物的实验是该P01的定义特征。这三个项目都需要对心脏进行分析在新生儿或成年猪中的功能，但是住房，外科和分析要求太贵了在单个实验室中进行，通常需要专业知识，以使用专业和精致设备。因此，大动物核心（核心B）将为所有手术程序提供集中设施（例如，诱导心肌损伤，治疗给药，围手术期），心脏功能评估（磁共振成像[MRI]，血液动力学）和电生理评估（编程的电刺激，植入环记录器，光学映射）。核心B将位于阿拉巴马大学伯明翰的心律管理实验室（CRML），动物将被安置在邻近的动物资源计划（ARP）中。总的来说，这两个设施都有所有进行我们提出的实验所需的资源和设备，并由异常人员组成熟练，高效且经验丰富的研究人员，心脏外科医生，兽医和技术人员。这核心B的责任是在以下三个特定目标（SAS）下组织的：SA 1，归纳新生儿和成年猪的心肌损伤； SA 2，实验疗法的递送（即移植工程化的人体心肌组织斑块[HMTPS]或最先进的[AAV-或ModRNA介导的]基因送货）; SA 3，电生理和功能评估。核心B还将收集组织准备生物样品进行组织学和分子分析，协调组织和样本到所有项目，并确保所有实验数据，协议，报告和其他文档均为在项目和核心之间牢固存储，存档和共享。值得注意的是，所有实验程序将是严格且始终如一地定义和执行所有项目，这将加速科学的步伐通过使我们能够从整个实验范围的比较中得出有效的结论来发现有效的结论在此PPG下进行。