Core B - Large Animal Core

核心B - 大型动物核心

基本信息

批准号：
10677723
负责人：
GREGORY P WALCOTT
金额：
$ 48.89万
依托单位：
UNIVERSITY OF ALABAMA AT BIRMINGHAM
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-09-01 至 2027-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10677723
关键词：
Acceleration Adult Alabama Animal Model Animals Archives Arrhythmia Bioinformatics Cardiac Cardiac Myocytes Cell Cycle Complex Data Data Analyses Documentation Echocardiography Electric Stimulation Electrophysiology (science)Ensure Equipment Evaluation Family suidae Gene Delivery Housing Human Engineering Human Resources Implant Individual Injury Investigational Therapies Laboratories Leadership Magnetic Resonance Imaging Mammals Maps Mediating Methods Modeling Molecular Analysis Monitor Muscle Myocardial Myocardial tissue Neonatal Operative Surgical Procedures Optics Perioperative Care Predisposition Procedures Process Proliferating Protocols documentation Regulator Genes Reporting Reproducibility Research Personnel Resources Sampling Secure Site Structure Surgeon Tissues Training Transplantation Universities Ventricular Veterinarians animal resource biological preparation cost data quality experience experimental study good laboratory practice heart function heart rhythm hemodynamics histological specimens in vivo myocardial injury operation programs protocol development skills treatment effect

项目摘要

ABSTRACT (Core B) Large Animal Model and Electrophysiology Core Experiments in large mammals are a defining feature of this P01. All three projects require analyses of cardiac function in neonatal or adult pigs, but the housing, surgical, and analytical requirements are too expensive to be conducted in an individual laboratory and typically require expertise in the use of specialized and sophisticated equipment. Thus, the Large Animal Core (Core B) will provide a centralized facility for all surgical procedures (e.g., induction of myocardial injury, treatment administration, perioperative care), cardiac functional assessments (magnetic resonance imaging [MRI], hemodynamics), and electrophysiological evaluations (programmed electrical stimulation, implanted loop recorders, optical mapping). Core B will be located at the University of Alabama at Birmingham in the Cardiac Rhythm Management Laboratory (CRML), and animals will be housed at the adjacent Animal Resource Program (ARP). Collectively, these two facilities have all of the resources and equipment needed to conduct our proposed experiments and are staffed by an exceptionally skilled, efficient, and experienced team of investigators, cardiac surgeons, veterinarians, and technicians. The responsibilities of Core B are organized under the following three Specific Aims (SAs): SA 1, Induction of myocardial injury in neonatal and adult pigs; SA 2, Delivery of experimental therapy (i.e., transplantation of engineered human myocardial tissue patches [hMTPs] or state-of-the-art [AAV- or modRNA-mediated] gene delivery); and SA 3, Electrophysiological and functional assessments. Core B will also collect tissues, prepare biological samples for histological and molecular analysis, coordinate the shipment of tissues and samples to all Projects, and ensure that all experimental data, protocols, reports, and other documentation are securely stored, archived, and shared among Projects and Cores. Notably, all experimental procedures will be rigorously and consistently defined and executed for all Projects, which will accelerate the pace of scientific discovery by enabling us to draw valid conclusions from comparisons across the full range of experiments conducted under this PPG.

抽象的（核心B）大型动物模型和电生理学核心大型哺乳动物实验是 P01 的一个显着特征。所有三个项目都需要进行心脏分析在新生猪或成年猪中发挥功能，但饲养、手术和分析要求太昂贵，无法实现在单独的实验室中进行，通常需要使用专门和复杂的技术的专业知识设备。因此，大型动物核心（核心 B）将为所有外科手术提供集中设施（例如，诱导心肌损伤、治疗管理、围手术期护理）、心脏功能评估（磁共振成像 [MRI]、血流动力学）和电生理学评估（编程电刺激、植入循环记录器、光学测绘）。核心 B 将位于阿拉巴马大学伯明翰分校的心律管理实验室 (CRML)，动物将安置在邻近的动物资源计划 (ARP)。总的来说，这两个设施拥有所有进行我们提议的实验所需的资源和设备，并配备了非常出色的人员技术精湛、高效且经验丰富的研究人员、心脏外科医生、兽医和技术人员团队。这核心 B 的职责根据以下三个具体目标 (SA) 进行组织： SA 1，归纳新生猪和成年猪的心肌损伤； SA 2，实验性治疗的实施（即移植工程化人类心肌组织斑块 [hMTP] 或最先进的 [AAV 或 modRNA 介导的] 基因送货）; SA 3，电生理学和功能评估。核心B也会收集组织，准备用于组织学和分子分析的生物样品，协调组织的运输和所有项目的样品，并确保所有实验数据、协议、报告和其他文件在项目和核心之间安全地存储、存档和共享。值得注意的是，所有实验程序都将所有项目都严格一致地定义和执行，这将加快科学发展的步伐通过使我们能够从全方位实验的比较中得出有效的结论来发现根据本 PPG 进行。