Latitudinal Landscape Genomics and Ecology of Anopheles Darlingi

达林按蚊纬度景观基因组学和生态学

• 批准号: 10674033
• 负责人: Jan E Conn
• 金额: $ 40.15万
• 依托单位: WADSWORTH CENTER
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-15 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10674033
• 关键词: Affect; Agriculture; Anopheles Genus; Anopheles gambiae; Atlases; Automobile Driving; Blood; Brazil; Case Study; Categories; Characteristics; Chromosome Mapping; Collection; Copy Number Polymorphism; Country; Coupled; Coupling; DNA Resequencing; Data; Deforestation; Demography; Ecology; Economic Development; Elements; Evolution; Female; Genes; Genetic; Genetic Drift; Genetic Markers; Genetic Polymorphism; Genetic Variation; Genome; Genomics; Geographic Locations; Goals; Gold; Habitats; Household; Human; Human Bites; Individual; Interruption; Intervention; Knowledge; Latin America; Link; Maintenance; Malaria; Measurable; Measures; Mining; Nature; Nucleotides; Panama; Parasitic Diseases; Pattern; Population; Population Genetics; Population Sizes; Precipitation; Prevalence; Proliferating; Public Health; Rain; Reporting; Resistance; Rest; Risk; Risk Factors; Role; Rural; Sampling; Seasons; Signal Transduction; Single Nucleotide Polymorphism; Socioeconomic Factors; South America; Structure; Surveys; Temperature; Testing; Variant; Vectorial capacity; Venezuela; anthropogenesis; forest; genome sequencing; genome-wide; genomic signature; improved; indexing; insight; land cover; land use; life history; malaria transmission; mosquito-borne; parity; pressure; public health relevance; reference genome; regional difference; socioeconomics; sugar; transmission process; vector; whole genome

Project Summary Despite major progress in malaria reduction in Latin America from 2005-2015, malaria cases have again increased to nearly one million in the last few years, with 75% of these cases reported in Venezuela and Brazil. We emphasize that ecological variables (such as ecotones and land use classes) that impact the primary neotropical vector Anopheles darlingi (in Brazil and Venezuela) and the regional vector An. albitarsis s.l. (equal in importance to An. darlingi in Venezuela) are largely unquantified or unidentified, constituting a major information gap. The premise of the proposed study is that human-modified landscape types (riverine, mining, agricultural) in persistent malaria hotspots differ significantly in measurable determinants of transmission (ecological, entomological, socioeconomic). Quantification of these determinants in landscape types in Venezuela and Brazil is essential for malaria surveillance to facilitate local targeted interventions to most effectively reduce transmission. Coupling these measures with landscape genomics, we propose to identify and quantify, in a spatially explicit way, landscape features affecting microevolution of malaria vectors. This will provide new insights into local vector adaptation in heterogeneous landscapes, and will help determine why neotropical malaria hotspots persist, at times over multiple years, in certain geographic locations. We hypothesize that the ecological drivers of abundance, proliferation and survival in An. darlingi and An. albitarsis s.l. will differ significantly due to unique ecological signatures (niches) of each species. We anticipate that our findings will provide new information about differential habitat suitability that can be applied throughout the broad and largely overlapping distributions of An. darlingi and An. albitarsis s.l. Polymorphism discovery through whole genome resequencing, integrated with measures of entomological and socioeconomic factors that intensify human-vector contact, will reveal the evolutionary genetic basis of factors promoting vector proliferation and connectivity among vector populations, allowing for more effective vector surveillance. A key remaining issue in our understanding of the role of An. darlingi landscape genomics in malaria transmission is whether gene flow contributes to rapid adaptation and colonization of deforested/degraded habitats throughout the Amazon Basin. We will employ landscape genomics at a regional scale in both Brazil and Venezuela to test whether An. darlingi maintains genetic connectivity or is isolated by resistance across ecotones punctuated by high forest cover. Whereas population genetic surveys based on RADseq or individual genes can detect overall population structure, whole genome sequencing studies have shown that these limited surveys may miss important patterns of ancestry, gene flow and evidence of adaptation (Anopheles gambiae 1000 Genomes Consortium 2017). Herein we present an integrated approach to identify and quantify ecological, entomological and socioeconomic drivers of malaria transmission, based on multi-scale analysis of An. darlingi and An. albitarsis s.l. collected under natural field conditions.

项目摘要 尽管从2005年至2015年开始，拉丁美洲疟疾降低了巨大进展，但疟疾病例又有了 在过去的几年中，增加到近100万，其中75％在委内瑞拉和巴西报告。 我们强调，影响主要的生态变量（例如生态变量和土地利用类别） 新热带媒介围绕着Darlingi（在巴西和委内瑞拉）和区域矢量。 Albitarsis S.L. （平等的 重要性对于一个。委内瑞拉的达令（Darlingi 信息差距。拟议的研究的前提是人类改造的景观类型（河流，采矿，， 持续性疟疾热点的农业）在可测量的传播决定因素上有显着差异 （生态，昆虫学，社会经济）。在景观类型中的这些决定因素的量化 委内瑞拉和巴西对于疟疾监视至关重要 有效减少传输。将这些措施与景观基因组学结合，我们建议确定 并以空间的方式量化影响疟疾载体微观进化的景观特征。这会 对异质景观的本地矢量适应提供新的见解，并将有助于确定为什么 在某些地理位置，新热带疟疾热点有时会在多年内持续存在。我们 假设An的生态驱动因素，增殖和生存的生态驱动因素。达令和。白质 S.L.由于每个物种的独特生态特征（壁ch），将大大差异。我们预计我们的 调查结果将提供有关差异栖息地适用性的新信息，可以在整个过程中应用 广泛的，很大程度上重叠的分布。达令和。 Albitarsis S.L.多态性发现 通过整个基因组重新纠正，与昆虫学和社会经济因素的度量融合在一起 加剧人类载体的接触将揭示促进载体因素的进化遗传基础 矢量种群之间的增殖和连通性，从而可以进行更有效的矢量监视。钥匙 在我们理解AN的作用时仍然存在问题。疟疾传播中的darlingi景观基因组学是 基因流是否有助于整个栖息地的快速适应和定植 亚马逊盆地。我们将在巴西和委内瑞拉的区域范围内采用景观基因组学 测试是否达令维利保持遗传连通性或通过跨ecotone抗性隔离 高森林覆盖物打断。而种群基于RADSEQ或单个基因的遗传调查 可以检测总体结构，整个基因组测序研究表明，这些限制 调查可能会错过祖先，基因流量和适应证据的重要模式（冈比亚山脉 1000个基因组财团2017）。本文我们提出了一种识别和量化的综合方法 基于多尺度分析的疟疾传播的生态，昆虫学和社会经济驱动因素 一个。达令和。 Albitarsis S.L.在自然野外条件下收集。

项目成果

Minimal genetic differentiation of the malaria vector Nyssorhynchus darlingi associated with forest cover level in Amazonian Brazil.

疟疾病媒 Nyssorhynchus darlingi 的最小遗传分化与巴西亚马逊流域森林覆盖水平相关。

• DOI: 10.1371/journal.pone.0225005
• 发表时间: 2019
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Prussing,Catharine;Emerson,KevinJ;Bickersmith,SaraA;Sallum,MariaAniceMureb;Conn,JanE
• 通讯作者: Conn,JanE

Molecular operational taxonomic units of mosquitoes (Diptera: Culicidae) collected in high Andean mountain ecosystems of Antioquia, Colombia.

在哥伦比亚安蒂奥基亚安第斯山脉高山生态系统中收集的蚊子（双翅目：蚊科）的分子操作分类单位。

• DOI: 10.11646/zootaxa.4277.3.3
• 发表时间: 2017
• 期刊: Zootaxa
• 影响因子: 0.9
• 作者: Rosero-garcÍa,Doris;Bickersmith,SaraA;Suaza-Vasco,JuanDavid;Porter,Charles;Correa,MargaritaM;Conn,JanE;Uribe-Soto,Sandra
• 通讯作者: Uribe-Soto,Sandra

Decreasing proportion of Anopheles darlingi biting outdoors between long-lasting insecticidal net distributions in peri-Iquitos, Amazonian Peru.

在秘鲁亚马逊流域伊基托斯周边地区，长期部署的杀虫网分布期间，达林按蚊在户外叮咬的比例有所下降。

• DOI: 10.1186/s12936-018-2234-4
• 发表时间: 2018
• 期刊: Malaria journal
• 影响因子: 3
• 作者: Prussing,Catharine;Moreno,Marta;Saavedra,MarlonP;Bickersmith,SaraA;Gamboa,Dionicia;Alava,Freddy;Schlichting,CarlD;Emerson,KevinJ;Vinetz,JosephM;Conn,JanE
• 通讯作者: Conn,JanE

Evidence for temporal population replacement and the signature of ecological adaptation in a major Neotropical malaria vector in Amazonian Peru.

• DOI: 10.1186/s12936-015-0863-4
• 发表时间: 2015-09-29
• 期刊: Malaria journal
• 影响因子: 3
• 作者: Lainhart W;Bickersmith SA;Nadler KJ;Moreno M;Saavedra MP;Chu VM;Ribolla PE;Vinetz JM;Conn JE
• 通讯作者: Conn JE

Malaria in Southern Venezuela: The hottest hotspot in Latin America.

• DOI: 10.1371/journal.pntd.0008211
• 发表时间: 2021-01
• 期刊: PLoS neglected tropical diseases
• 影响因子: 3.8
• 作者: Grillet ME;Moreno JE;Hernández-Villena JV;Vincenti-González MF;Noya O;Tami A;Paniz-Mondolfi A;Llewellyn M;Lowe R;Escalante AA;Conn JE
• 通讯作者: Conn JE