Non-Human Primates

非人类灵长类动物

• 批准号: 8829139
• 负责人: Gunilla Karlsson Hedestam
• 金额: $ 50.33万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8829139
• 关键词: Address; Animal Welfare; Animals; Antibodies; Antigens; Arts; B-Lymphocytes; Back; Biopsy; Bone Marrow; Caring; Cells; Clinical; Collaborations; Communication; Coupled; DNA; Decision Making; Dose; Ensure; Ethics; Freezing; Generations; Genetic; Goals; HIV-1; Hand; Hemorrhage; Housing; Human; Human Resources; Immune response; Immunology; Instruction; Laboratories; Laboratory Personnel; Link; Liquid substance; Lymphocyte; Macaca mulatta; Methods; Nitrogen; Pathogenesis; Plasma Cells; Population; Preparation; Primates; Process; Protocols documentation; Publications; Resolution; Risk Assessment; Sampling; Schedule; Services; Sweden; Time; United States National Institutes of Health; Update; Vaccine Adjuvant; Vaccine Antigen; Vaccine Design; Vaccines; Vial device; Work; animal facility; biobank; design; env Glycoproteins; immunogenicity; improved; lymph nodes; meetings; nonhuman primate; professor; research study; response; sample collection; success; vaccine candidate; vaccine evaluation; vaccine trial

Studies in non-human primates (NHPs) have made substantial contributions to our understanding of HIV-1 pathogenesis and vaccine immunology. The high overall genetic homology between humans and rhesus macaques, coupled with the phenotypic conservation of lymphocyte populations, highlights the utility of NHPs for evaluating clinical vaccine candidates. Core B will be responsible for the planning, design and execution of non-human primate (NHP) experiments described in Projects 1-4 of this HIVRAD P01. Additionally, the Core will support the dissemination of NHP cells, plasma, DNA and cloned antibodies to Project 1-4 as required to meet the overall goals ofthe HIVRAD consortia. In addition, the Core will support sample distribution to relevant collaboration partners, to maximize the information gained from these studies. In Aim 1, Core B will provide the necessary logistical support for the NHP experiments such as complete the appropriate papenwork for conducting NHP studies at the Astrid Fagraeus Laboratory (AFL) in Stockholm, plan the purchase and transport ofthe rhesus macaques and their housing organization. Core B will also serve as a communication link between Projects 1-4 and the NIH for all issues related to the NHP immunogenicity studies to support the efforts of all Projects within the proposal. In Aim 2, Core B will oversee the execution ofthe NHP experiments to be conducted in this proposal, including ensuring that detailed schedules for inoculations and sample collection exist as well as robust processes to maintain the NHP biobank. In Aim 3, Core B will work with the personnel at the AFL to refine methods for B cell sampling and analysis of humoral immune responses. The proximity between the AFL and the Karisson Hedestam laboratory means that vaccine components can be prepared immediately before animal inoculation and hand-delivered directly to the AFL. Similariy, transfer of cells from the animals back to the laboratory is performed by personnel in the Karlsson Hedestam laboratory who collect PBMCs, bone marrow, lymph node biopsies directly from the AFL for direct analysis or freezing back in the Karisson Hedestam laboratory. These processes are of critical importance forthe success ofthe proposed HIVRAD project.

非人类灵长类动物 (NHP) 的研究为我们了解 HIV-1 做出了重大贡献 发病机制和疫苗免疫学。人类和恒河猴之间的整体遗传同源性很高 猕猴，加上淋巴细胞群体的表型保守，强调了 用于评估临床候选疫苗的 NHP。核心B将负责规划、设计和 执行本 HIVRAD P01 项目 1-4 中描述的非人类灵长类动物 (NHP) 实验。 此外，该核心还将支持 NHP 细胞、血浆、DNA 和克隆抗体的传播 项目 1-4 根据需要满足 HIVRAD 联盟的总体目标。此外，该核心还将支持 将样本分发给相关合作伙伴，以最大限度地利用从这些研究中获得的信息。 在目标 1 中，核心 B 将为 NHP 实验提供必要的后勤支持，例如完成 在斯德哥尔摩的 Astrid Fagraeus 实验室 (AFL) 进行 NHP 研究的适当文书工作， 规划恒河猴的购买和运输及其住房组织。核心B也将 作为项目 1-4 和 NIH 之间关于 NHP 相关所有问题的沟通纽带 免疫原性研究，以支持提案内所有项目的努力。在目标 2 中，核心 B 将监督 本提案中将进行的 NHP 实验的执行，包括确保详细的 现有接种和样本采集时间表以及维护 NHP 的稳健流程 生物样本库。在目标 3 中，Core B 将与 AFL 的人员合作，完善 B 细胞采样和 体液免疫反应的分析。 AFL 和 Karisson Hedestam 之间的距离很近 实验室意味着可以在动物接种前立即制备疫苗成分 直接手工交付给 AFL。类似地，将细胞从动物转移回实验室是 由 Karlsson Hedestam 实验室的人员进行，收集 PBMC、骨髓、淋巴结 直接从 AFL 进行活检进行直接分析或冷冻回 Karisson Hedestam 实验室。 这些过程对于拟议的 HIVRAD 项目的成功至关重要。