Cellular and Molecular Imaging Core

细胞和分子成像核心

基本信息

批准号：
10666523
负责人：
Alexander Martinus Van der Linden
金额：
$ 12.51万
依托单位：
UNIVERSITY OF NEVADA RENO
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-07-15 至 2027-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10666523
关键词：
3-Dimensional Acceleration Administrative Supplement Animals Automation Award Biology Cell Separation Cells Cellular biology Centers of Research Excellence Consult Contract Services Core Facility DNA Data Data Collection Detection Development Disease Education Emerging Technologies Ensure Environment Equipment Extramural Activities Faculty Fee-for-Service Plans Fees Funding Gel Goals Grant Human Resources Hybrids Image Imaging Device Imaging technology Individual Institution Knowledge Laboratories Microfluidics Microscope Microscopy Modeling Modernization Molecular Molecular Biology Techniques Nervous System Physiology Neurosciences Neurosciences Research Nevada Organ Organism Phase Pilot Projects Postdoctoral Fellow Principal Investigator Process Productivity Proteins Publications RNA Research Research Infrastructure Research Personnel Research Priority Research Support Resources Robot Sampling Services Specialist Structure Students Technology Tilia Time Tissues Training Training and Education Translating Universities Visualization Wages base cellular imaging cost effective imager imaging platform instrument microscopic imaging model organism molecular imaging nervous system development nervous system disorder operation parent grant programs recruit satisfaction success technology platform tissue culture tool two-photon ultra high resolution voucher

项目摘要

Program Director/Principal Investigator (Last, First, Middle): Michael A. Webster PROJECT SUMMARY/ABSTRACT Cellular and Molecular Imaging Core The goal of the Phase III Cellular and Molecular Imaging (CMI) Core is to provide COBRE investigators at the University of Nevada, Reno (UNR) state-of-the-art imaging tools and training in neuroscience-oriented research. The Core provides equipment and expertise in two-photon, confocal and wide-field Thunder microscopy with advanced computational capabilities in the visualization, quantification and interpretation of cells, tissues, whole organs, and small animals. The Core also provides molecular imaging equipment for the detection, quantification, and analysis of biomolecular samples such as DNA, RNA, and protein. The Core will assist investigators with collecting image data and analysis to further their research, facilitate publication and to enhance their competitiveness for extramural grant funding. The Core equipment is centrally located in the Biology Department, in close proximity to COBRE investigators, and has successfully developed a wide user base (19 research labs and counting) with high level of interactions among users. The Core will be directed by Dr. van der Linden, who developed the core in Phase II. Building on its success during Phase II, the Core will expand and accelerate research productivity of COBRE investigators and others with the following specific aims: 1) To provide continued access and technical support to investigators whose research requires the acquisition of high-quality microscopy and molecular data using our existing and recently added technologies and imaging platforms. The Core will also leverage a modern renovated Tissue Culture facility supported by the former Cell Biology COBRE center; 2) To provide access, training and education in cellular and molecular imaging research and resources, together with imaging expertise and experimental assistance needed for image data collection, analysis, and interpretation. As such, the Core will recruit a new Imaging Specialist to meet the increasing needs of microscopy imaging; 3) To establish cost-effective services towards a sustainable core facility. In sum, CMI Core resources and services will create a path for COBRE investigators and others to exploit advanced imaging technologies, allowing them to take their research into new directions and discoveries with an emphasis on gaining fundamental knowledge in nervous system function and development, and its associated diseases. OMB No. 0925-0001/0002 (Rev. 03/2020 Approved Through 02/28/2023) Page Continuation Format Page

计划主任/首席研究员（最后，第一，中间）：迈克尔·A·韦伯斯特项目摘要/摘要细胞和分子成像核心 III期细胞和分子成像（CMI）核心的目的是为毛病研究者提供内华达大学，里诺（UNR）的最先进的成像工具和面向神经科学的研究的培训。该核心提供了两光子，共聚焦和广阔的雷电显微镜的设备和专业知识高级计算能力在可视化，定量和解释细胞，组织，整个中器官和小动物。核心还提供了用于检测，定量的分子成像设备分析生物分子样品，例如DNA，RNA和蛋白质。核心将协助调查人员收集图像数据和分析以进一步研究，促进出版并增强其授权授予资助的竞争力。核心设备位于生物学系中心靠近毛茸茸的调查员，并成功地开发了一个广泛的用户群（19个研究实验室和计数）用户之间的交互水平很高。核心将由Van der Linden博士执导，他在第二阶段发展了核心。基于第二阶段的成功，核心将扩展和加速具有以下特定目的的毛to研究者和其他人的研究生产率：1）持续对研究人员的访问和技术支持，他们的研究需要获取高质量显微镜以及分子数据，使用我们现有的和最近添加的技术和成像平台。核心意愿还利用了由以前的细胞生物学中心支持的现代化的经过翻新的组织培养设施； 2）提供有关细胞和分子成像研究和资源的访问，培训和教育，一起图像数据收集，分析和解释。因此，核心将招募新的成像专家以满足显微镜的需求不断增长成像； 3）为可持续核心设施建立具有成本效益的服务。总之，CMI核心资源服务将为毛绒研究人员和其他人创造一条途径，以利用高级成像技术，允许他们将研究带入新的方向和发现，重点是获得神经系统功能和发育及其相关疾病的基本知识。 OMB No. 0925-0001/0002（Rev. 03/2020通过02/28/2023批准）页面延续格式页面