Cellular and Molecular Imaging Core

细胞和分子成像核心

基本信息

批准号：
10666523
负责人：
Alexander Martinus Van der Linden
金额：
$ 12.51万
依托单位：
UNIVERSITY OF NEVADA RENO
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-07-15 至 2027-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10666523
关键词：
3-Dimensional Acceleration Administrative Supplement Animals Automation Award Biology Cell Separation Cells Cellular biology Centers of Research Excellence Consult Contract Services Core Facility DNA Data Data Collection Detection Development Disease Education Emerging Technologies Ensure Environment Equipment Extramural Activities Faculty Fee-for-Service Plans Fees Funding Gel Goals Grant Human Resources Hybrids Image Imaging Device Imaging technology Individual Institution Knowledge Laboratories Microfluidics Microscope Microscopy Modeling Modernization Molecular Molecular Biology Techniques Nervous System Physiology Neurosciences Neurosciences Research Nevada Organ Organism Phase Pilot Projects Postdoctoral Fellow Principal Investigator Process Productivity Proteins Publications RNA Research Research Infrastructure Research Personnel Research Priority Research Support Resources Robot Sampling Services Specialist Structure Students Technology Tilia Time Tissues Training Training and Education Translating Universities Visualization Wages base cellular imaging cost effective imager imaging platform instrument microscopic imaging model organism molecular imaging nervous system development nervous system disorder operation parent grant programs recruit satisfaction success technology platform tissue culture tool two-photon ultra high resolution voucher

项目摘要

Program Director/Principal Investigator (Last, First, Middle): Michael A. Webster PROJECT SUMMARY/ABSTRACT Cellular and Molecular Imaging Core The goal of the Phase III Cellular and Molecular Imaging (CMI) Core is to provide COBRE investigators at the University of Nevada, Reno (UNR) state-of-the-art imaging tools and training in neuroscience-oriented research. The Core provides equipment and expertise in two-photon, confocal and wide-field Thunder microscopy with advanced computational capabilities in the visualization, quantification and interpretation of cells, tissues, whole organs, and small animals. The Core also provides molecular imaging equipment for the detection, quantification, and analysis of biomolecular samples such as DNA, RNA, and protein. The Core will assist investigators with collecting image data and analysis to further their research, facilitate publication and to enhance their competitiveness for extramural grant funding. The Core equipment is centrally located in the Biology Department, in close proximity to COBRE investigators, and has successfully developed a wide user base (19 research labs and counting) with high level of interactions among users. The Core will be directed by Dr. van der Linden, who developed the core in Phase II. Building on its success during Phase II, the Core will expand and accelerate research productivity of COBRE investigators and others with the following specific aims: 1) To provide continued access and technical support to investigators whose research requires the acquisition of high-quality microscopy and molecular data using our existing and recently added technologies and imaging platforms. The Core will also leverage a modern renovated Tissue Culture facility supported by the former Cell Biology COBRE center; 2) To provide access, training and education in cellular and molecular imaging research and resources, together with imaging expertise and experimental assistance needed for image data collection, analysis, and interpretation. As such, the Core will recruit a new Imaging Specialist to meet the increasing needs of microscopy imaging; 3) To establish cost-effective services towards a sustainable core facility. In sum, CMI Core resources and services will create a path for COBRE investigators and others to exploit advanced imaging technologies, allowing them to take their research into new directions and discoveries with an emphasis on gaining fundamental knowledge in nervous system function and development, and its associated diseases. OMB No. 0925-0001/0002 (Rev. 03/2020 Approved Through 02/28/2023) Page Continuation Format Page

项目总监/首席研究员（最后、第一、中间）：Michael A. Webster 项目概要/摘要细胞和分子成像核心 III 期细胞和分子成像 (CMI) 核心的目标是为 COBRE 研究人员提供内华达大学里诺分校 (UNR) 最先进的成像工具和面向神经科学的研究培训。核心提供双光子、共焦和宽视场雷电显微镜的设备和专业知识细胞、组织、整体的可视化、量化和解释方面的先进计算能力器官和小动物。该核心还提供分子成像设备，用于检测、定量、以及 DNA、RNA 和蛋白质等生物分子样品的分析。核心将协助调查人员收集图像数据和分析以进一步研究、促进出版并提高他们的能力外部赠款资金的竞争力。核心设备位于生物系的中心，与 COBRE 研究人员非常接近，并已成功发展了广泛的用户群（19 个研究实验室和计数），用户之间具有高水平的交互。核心将由范德林登博士执导，他在第二阶段开发了核心。在第二阶段的成功基础上，核心将扩大并加速 COBRE 研究人员和其他人员的研究生产力，其具体目标如下： 1) 提供持续的为研究需要获得高质量显微镜的研究人员提供访问和技术支持和分子数据使用我们现有的和最近添加的技术和成像平台。核心将还利用由前细胞生物学 COBRE 中心支持的现代化翻新组织培养设施； 2) 共同提供细胞和分子成像研究和资源的访问、培训和教育具有图像数据收集、分析和分析所需的成像专业知识和实验协助解释。因此，核心将招募一名新的成像专家，以满足不断增长的显微镜需求成像； 3) 建立具有成本效益的服务，以实现可持续的核心设施。总而言之，CMI 核心资源和服务将为 COBRE 研究人员和其他人利用先进的成像技术创造一条道路，使他们能够将研究带入新的方向和发现，重点是获得神经系统功能和发育及其相关疾病的基础知识。 OMB 编号 0925-0001/0002（修订版 03/2020 已批准至 02/28/2023）页面延续格式页面