Supplement for temporal bone histological tissue scanning equipment

颞骨组织学组织扫描设备补充品

• 批准号: 10671303
• 负责人: Andrew J. Oxenham
• 金额: $ 22.15万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-03-01 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10671303
• 关键词: Accidents; Acoustics; Affect; Age; Archives; Auditory; Auditory Perception; Behavioral; Bone Tissue; Clinical; Clinical Research; Cochlea; Cochlear Implants; Cochlear Nerve; Collection; Communities; Data; Databases; Distal; Equipment; Funding Opportunities; Future; Goals; Hair Cells; Hearing; Hearing Aids; Histologic; Housing; Human; Image; Institution; Laboratories; Labyrinth; National Institute on Deafness and Other Communication Disorders; Natural Disasters; Nerve Fibers; Neurons; Organ; Parents; Pathologic; Patients; Performance; Property; Registries; Research; Research Personnel; Resolution; Resources; Scanning; Slide; Specimen; Speech Perception; Stria Vascularis; Temporal bone structure; Tissues; Universities; biological specimen archives; digital; digital repositories; hearing impairment; high resolution imaging; improved; interest; meetings; middle ear; neuroimaging; parent grant; parent project; relating to nervous system; searchable database; sex; spiral ganglion

Project summary This proposal is for the purchase of temporal bone histological tissue scanning equipment as a supplement to the parent proposal, R01 DC012262, entitled “Spectro-temporal interactions in electric and acoustic auditory perception”. The long-term goal of the parent proposal is to improve auditory and speech perception via cochlear implants (CIs) and hearing aids, through a greater understanding of the basic mechanisms that contribute to, and limit, the perception of speech in challenging acoustic conditions. The long-term goals of the parent project will benefit greatly from the use of otopathological specimens. Our university is fortunate to have one of the three largest human temporal bone laboratories in the US, housing a well-diversified collection of 2,252 human temporal bones. Our plan is to use digitized human temporal bone slides to relate the behavioral and neuroimaging results gathered under the parent grant with otopathological data, providing a unique opportunity to relate our perceptual findings to the cellular level within the inner ear and thereby assess the pathological underpinnings of altered speech perception. Our plan is to search the database of our otopathology laboratory and select specimens of special interest to our research (from patients who have similar background as ones in our clinical study: age- and sex- matched, with and without hearing loss). These specimens will be scanned using a high-resolution, high-throughput tissue scanner, which will be acquired through this funding opportunity (Zeiss Axioscan 7). We will then scrutinize the middle and inner ears of temporal bones for properties that relate to the perceptual performance of patients with CIs and hearing aids. We will perform quantitative analysis of cochlear hair cells, stria vascularis, spiral ganglion neurons, and dendritic distal cochlear nerve fibers. The benefits of having digital versions of the archival temporal bones will extend far beyond the parent project and beyond researchers at our institution, as these digital versions will protect the information from these irreplaceable specimens from being damaged in the case of accidents or natural disasters. In addition, by making the digitized collection of archival specimens widely available, they will become an invaluable long-term resource to the wider clinical and scientific communities involved with auditory and temporal bone research, meeting one of the fundamental objectives of the NIDCD Registry. The images will ultimately be made available to the scientific community through NIDCD approved databases.

项目概要 本提案为采购颞骨组织学组织扫描设备，作为对 父提案 R01 DC012262，标题为“电声听觉中的谱时相互作用” 家长提案的长期目标是通过人工耳蜗改善听觉和言语感知。 植入物（CI）和助听器，通过更好地了解有助于的基本机制， 和限制，在具有挑战性的声学条件下的语音感知是父项目的长期目标。 将从使用耳病理学标本中受益匪浅，我们大学很幸运拥有这三个标本之一。 美国最大的人类颞骨实验室，拥有 2,252 名多样化的人类标本 我们的计划是使用数字化的人类颞骨幻灯片来关联行为和行为。 在家长资助下收集的神经影像结果和耳病理学数据，提供了独特的机会 将我们的感知发现与内耳内的细胞水平联系起来，从而评估病理学 我们的计划是搜索我们的耳病理学实验室的数据库。 并选择我们的研究特别感兴趣的标本（来自与其他国家有相似背景的患者） 我们的临床研究：年龄和性别匹配，有或没有听力损失）。 使用高分辨率、高通量组织扫描仪，该扫描仪将通过本次融资机会获得 （Zeiss Axioscan 7）然后我们将检查颞骨的中耳和内耳的相关属性。 我们将对佩戴 CI 和助听器的患者的感知表现进行定量分析。 耳蜗毛细胞、血管纹、螺旋神经节神经元和树突状远端耳蜗神经纤维。 拥有档案颞骨数字版本的好处将远远超出父项目的范围 超出了我们机构的研究人员的范围，因为这些数字版本将保护信息免受这些人的侵害 不可替代的标本在发生事故或自然灾害时不会被损坏。 档案标本的数字化收藏广泛可用，它们将成为宝贵的长期资源 参与听觉和颞骨研究的更广泛的临床和科学界，会议一 NIDCD 登记处的基本目标是，这些图像最终将提供给 NIDCD 登记处。 科学界通过 NIDCD 批准的数据库。