Pulvinar cellular and network dynamics in cognitive control of sensory processes.

感觉过程认知控制中的枕细胞和网络动力学。

• 批准号: 10633806
• 负责人: W MARTIN USREY
• 金额: $ 48.52万
• 依托单位: PRINCETON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-04-03 至 2028-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10633806
• 关键词: Address; Affect; Animal Model; Animals; Area; Attention; Automobile Driving; Axon; Behavioral; Biological Assay; Brain; Brain imaging; Cerebral cortex; Cognition; Cognitive; Communication; Complement; Data; Decision Making; Disease; Electrodes; Ensure; Environment; Frequencies; Human; Laboratories; Lateral; Mediating; Mediator; Modeling; Monkeys; Neurons; Output; Pathway interactions; Periodicity; Peripheral; Play; Positioning Attribute; Process; Property; Pulvinar structure; Regulation; Role; Sensory; Sensory Process; Shock; Signal Transduction; Stimulus; Structure; Synapses; Testing; Thalamic Nuclei; Thalamic structure; Toes; Translating; Visual Cortex; analytical tool; area V1; attentional control; behavior measurement; behavioral study; biophysical model; cognitive control; cognitive task; experimental study; extrastriate visual cortex; human disease; imaging approach; neurotransmission; operation; optogenetics; response; retinotopic; sensory cortex; synergism

PROJECT 1 SUMMARY P1 is aligned with each of the Center’s 3 overarching aims: 1. Identify cognitive functional organization principles of higher order thalamic nuclei in interacting with cortex; 2. Translate thalamic functionality from animal models to healthy and diseased human brain; 3. Develop a biologically plausible model for human higher order thalamus. Specifically, P1 will investigate regions of the pulvinar associated with early visual cortical areas (V1, V2, and V4) to test specific hypotheses about cognition and circuit dynamics in higher order thalamus. This project takes advantage of the retinotopic relationship between pulvinar and cortex to enable precise electrode placement for studying the influence of cognitive control on circuit dynamics at a synaptic, cellular, and network level. In alignment with Center Aim 1, results from P1 will be compared to those from P2, P3, and P4 to establish a comprehensive and mechanistic understanding of the role of higher order thalamus in cognitive control. In alignment with Center Aim 2, results from P1 will provide a framework for understanding the impact of disease in (P5). In alignment with Center Aim 3, results from P1 will inform Core B as its models evolve. Core C will provide essential analytical tools for P1 as well as ensure that data can be exchanged and compared between projects. In P1/Aim 1, we will record from optogenetically identified regions of pulvinar associated with V1, V2, and V4 in monkeys performing the APU task. We will then optogenetically silence the V1 inputs to these PUL regions to assess their role in decision making and spatial attention. In P1/Aim 2, we will use an assay developed in our laboratory, the “dual-neuron shock-spike assay”, to determine the spatial organization of attention effects in the pulvinar and the influence of attention on the efficacy of pulvino-cortical communication. Lastly, in P1/Aim 3, we will use optogenetics to inactivate V1 and V2 axons within the pulvinar to determine the influence of cognitive factors on pulvinar regulation of cortico-cortical communication.

项目1摘要 P1与中心的3个总体目的一致：1。确定认知功能组织原理 高阶丘脑核与皮质相互作用； 2。翻译动物模型的丘脑功能 到健康的人脑； 3。为人类高阶丘脑开发一个生物学上合理的模型。 具体而言，P1将研究与早期视觉皮质区域相关的pulvinar区域（V1，V2和 v4）以高阶丘脑测试有关认知和电路动态的特定假设。这个项目需要 Pulvinar和Cortex之间的视网膜关系的优势使精确的电极放置 研究认知控制对突触，细胞和网络水平上电路动力学的影响。在 与中心AIM 1的对齐，将P1的结果与P2，P3和P4的结果进行比较，以建立一个 对高级丘脑在认知控制中的作用的全面和机械理解。在 与中心AIM 2的一致性，P1的结果将为理解疾病的影响提供一个框架 在（P5）中。在与中心AIM 3的一致性中，P1的结果将告知核心B作为模型的演变。 Core C Will 为P1提供基本的分析工具，并确保可以更改和比较数据 项目。 在P1/AIM 1中，我们将从与V1，V2和 V4在执行APU任务的猴子中。然后，我们将光源使V1输入到这些脉冲区域 评估他们在决策和空间关注中的作用。在P1/AIM 2中，我们将使用我们的评估 实验室，“双神经元冲击尖峰测定”，以确定注意力的空间组织 普尔维纳尔和注意力对普尔维诺 - 皮质通信效率的影响。最后，在P1/AIM 3中，我们 将使用光遗传学使Pulvinar内的V1和V2轴突灭活，以确定认知的影响 皮质皮质通信的脉冲调节因素。