Pulvinar cellular and network dynamics in cognitive control of sensory processes.

感觉过程认知控制中的枕细胞和网络动力学。

• 批准号: 10633806
• 负责人: W MARTIN USREY
• 金额: $ 48.52万
• 依托单位: PRINCETON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-04-03 至 2028-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10633806
• 关键词: Address; Affect; Animal Model; Animals; Area; Attention; Automobile Driving; Axon; Behavioral; Biological Assay; Brain; Brain imaging; Cerebral cortex; Cognition; Cognitive; Communication; Complement; Data; Decision Making; Disease; Electrodes; Ensure; Environment; Frequencies; Human; Laboratories; Lateral; Mediating; Mediator; Modeling; Monkeys; Neurons; Output; Pathway interactions; Periodicity; Peripheral; Play; Positioning Attribute; Process; Property; Pulvinar structure; Regulation; Role; Sensory; Sensory Process; Shock; Signal Transduction; Stimulus; Structure; Synapses; Testing; Thalamic Nuclei; Thalamic structure; Toes; Translating; Visual Cortex; analytical tool; area V1; attentional control; behavior measurement; behavioral study; biophysical model; cognitive control; cognitive task; experimental study; extrastriate visual cortex; human disease; imaging approach; neurotransmission; operation; optogenetics; response; retinotopic; sensory cortex; synergism

PROJECT 1 SUMMARY P1 is aligned with each of the Center’s 3 overarching aims: 1. Identify cognitive functional organization principles of higher order thalamic nuclei in interacting with cortex; 2. Translate thalamic functionality from animal models to healthy and diseased human brain; 3. Develop a biologically plausible model for human higher order thalamus. Specifically, P1 will investigate regions of the pulvinar associated with early visual cortical areas (V1, V2, and V4) to test specific hypotheses about cognition and circuit dynamics in higher order thalamus. This project takes advantage of the retinotopic relationship between pulvinar and cortex to enable precise electrode placement for studying the influence of cognitive control on circuit dynamics at a synaptic, cellular, and network level. In alignment with Center Aim 1, results from P1 will be compared to those from P2, P3, and P4 to establish a comprehensive and mechanistic understanding of the role of higher order thalamus in cognitive control. In alignment with Center Aim 2, results from P1 will provide a framework for understanding the impact of disease in (P5). In alignment with Center Aim 3, results from P1 will inform Core B as its models evolve. Core C will provide essential analytical tools for P1 as well as ensure that data can be exchanged and compared between projects. In P1/Aim 1, we will record from optogenetically identified regions of pulvinar associated with V1, V2, and V4 in monkeys performing the APU task. We will then optogenetically silence the V1 inputs to these PUL regions to assess their role in decision making and spatial attention. In P1/Aim 2, we will use an assay developed in our laboratory, the “dual-neuron shock-spike assay”, to determine the spatial organization of attention effects in the pulvinar and the influence of attention on the efficacy of pulvino-cortical communication. Lastly, in P1/Aim 3, we will use optogenetics to inactivate V1 and V2 axons within the pulvinar to determine the influence of cognitive factors on pulvinar regulation of cortico-cortical communication.

项目 1 摘要 P1 与中心的 3 个总体目标保持一致： 1. 确定认知功能组织原则 2. 从动物模型中翻译丘脑功能； 健康和患病的人类大脑； 3. 开发人类高级丘脑的生物学模型。 具体来说，P1 将研究与早期视觉皮质区域（V1、V2 和 V4）测试有关高阶丘脑认知和电路动力学的具体假设。 利用枕丘和皮质之间的视网膜专题关系，实现精确的电极放置 研究认知控制对突触、细胞和网络水平的回路动力学的影响。 与中心目标 1 保持一致，P1 的结果将与 P2、P3 和 P4 的结果进行比较，以确定 对高阶丘脑在认知控制中的作用的全面和机械的理解。 与中心目标 2 一致，P1 的结果将为理解疾病的影响提供一个框架 在（P5）中，与中心目标 3 一致，P1 的结果将随着其模型的发展而通知核心 B。 为 P1 提供必要的分析工具，并确保数据可以在 P1 之间交换和比较 项目。 在 P1/Aim 1 中，我们将从光遗传学鉴定的与 V1、V2 和 V1、V2 相关的 pulvinar 区域进行记录 执行 APU 任务的猴子中的 V4 然后我们将通过光遗传学沉默这些 PUL 区域的 V1 输入。 为了评估他们在决策和空间注意力中的作用，在 P1/Aim 2 中，我们将使用我们开发的分析方法。 实验室，“双神经元冲击尖峰测定”，以确定注意力效应的空间组织 最后，在 P1/Aim 3 中，我们讨论了枕髓和注意力对枕髓皮质沟通功效的影响。 将使用光遗传学使枕丘内的 V1 和 V2 轴突失活，以确定认知的影响 皮质-皮质通讯枕调节的因素。