Cell Isolation and Organ Function Core

细胞分离和器官功能核心

基本信息

批准号：
10630737
负责人：
Hongwei Yao
金额：
$ 26.97万
依托单位：
OCEAN STATE RESEARCH INSTITUTE, INC.
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-06-01 至 2028-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10630737
关键词：
Acute Respiratory Distress Syndrome Arterial Fatty Streak Asthma Atherosclerosis Biology Blood Vessels Bronchopulmonary Dysplasia COVID-19/ARDS Cardiac Cardiac Myocytes Cardiopulmonary Cell Separation Cell physiology Cells Centers of Research Excellence Collaborations Communities Congenital Heart Defects Consumption Coronary Coronary Arteriosclerosis Data Analyses Development Disease Endothelial Cells Endothelium Experimental Designs Fibroblasts Fostering Functional disorder Generations Goals Heart Heart Diseases Hypoxemia Individual Inflammation Injury Interruption Ischemia Lung Lung Diseases Measurement Mutation Myocardial Reperfusion Injury Natural regeneration Obstruction Organ Pathogenesis Perfusion Phase Pilot Projects Prevention Productivity Pulmonary Hypertension Reperfusion Injury Reproducibility Research Research Personnel Rhode Island Services Standardization Systemic hypertension Technical Expertise Techniques Technology Thrombosis Tissues Training Translating Vascular Diseases Vascular remodeling Vascular resistance Vascularization Vasomotor Work cost data acquisition idiopathic pulmonary fibrosis injury and repair insight instrument lung vascular injury pandemic disease programs quality assurance repaired response to injury restoration skills temporal measurement tool transcription factor vascular injury ventilation vessel regression

项目摘要

Cell Isolation and Organ Function (CIOF) Core The central theme of the CardioPulmonary Vascular Biology (CPVB) COBRE is to facilitate high impact vascular biology research and enable investigators to develop insights into basic mechanisms of vasculopathies and translate these findings into prevention or treatment for heart, lung, and vessel diseases. An increased understanding of the mechanisms regulating vascularization and repair may lead to unique approaches to interrupt disease pathogenesis or to promote or enhance cardiopulmonary vascular and vessel restoration. The Cell Isolation and Organ Function (CIOF) core provides a unique skill set and expertise to Rhode Island vascular biologists by providing quality assurance in isolation, characterization, and propagation of vascular derived cells and fibroblasts and cardiopulmonary ex vivo organ function analyses. The centralization of the cell and ex vivo organ isolation, characterization, and function measurements has helped by standardizing technique and reproducibility, and in turn provided investigators the technical expertise, thus, minimizing variability and providing uniformity in data acquisition for CPVB investigators. The services provided by the CIOF Core have permitted the Investigators to focus their efforts on aspects of their research endeavors related to experimental design, execution and interpretation. During Phase I and II, the CIOF core assisted 78 unique users, provided 5366 services, and performed ~$53,000 worth of services in year 9. Further, CIOF provided training for 36 individuals and provided data analyses for 370 requests (despite pandemic shutdown). The specific aims of the CIOF core are to: i) offer effective and reproducible services in cell isolation and ex vivo organ function; ii) acquire, establish, and disseminate technologies and instrument capabilities to provide state of the art research tools for the scientific community; iii) work with IDeA programs in Rhode Island (RI) and other states to foster scientific networks and collaborations by providing technical services to enhance research productivity.; and iv) maintain a robust line of services that meet the research needs of the scientific community. The CIOF core will continue to support and enhance vascular biology research, including for new pilot project investigators and the scientific community, in developing, facilitating, implementing, and performing technical services to impact the research productivity of our IDeA colleagues and collaborators.

细胞分离和器官功能 (CIOF) 核心心肺血管生物学 (CPVB) COBRE 的中心主题是促进高影响血管研究生物学研究，使研究人员能够深入了解血管病的基本机制和将这些发现转化为心脏、肺和血管疾病的预防或治疗。增加了了解调节血管形成和修复的机制可能会导致独特的方法中断疾病发病机制或促进或增强心肺血管和血管的恢复。这细胞分离和器官功能 (CIOF) 核心为罗德岛血管提供独特的技能和专业知识生物学家通过提供血管衍生细胞的分离、表征和繁殖的质量保证以及成纤维细胞和心肺离体器官功能分析。细胞的集中化和离体器官分离、表征和功能测量通过标准化技术和重现性，反过来又为研究人员提供了技术专业知识，从而最大限度地减少了变异性和为 CPVB 研究人员提供数据采集的一致性。 CIOF Core提供的服务有允许研究人员将精力集中在与实验相关的研究工作方面设计、执行和解释。在第一阶段和第二阶段，CIOF 核心协助了 78 个独立用户，前提是 5366 项服务，并在第 9 年提供了价值约 53,000 美元的服务。此外，CIOF 还为 36 名员工提供了培训个人并为 370 项请求提供了数据分析（尽管因大流行而关闭）。该计划的具体目标 CIOF 的核心是： i) 在细胞分离和离体器官功能方面提供有效且可重复的服务；二）获取、建立和传播技术和仪器能力，以提供最先进的研究科学界的工具； iii) 与罗德岛州 (RI) 和其他州的 IDeA 项目合作，促进通过提供技术服务来提高研究生产力，建立科学网络和合作。和四）维持强大的服务系列，以满足科学界的研究需求。 CIOF 核心将继续支持和加强血管生物学研究，包括新的试点项目研究人员和科学界在开发、促进、实施和提供技术服务以影响我们 IDeA 同事和合作者的研究生产力。