Metal Exposure and Early Cardiovascular Risk in Adult E-Cigarette Users

成人电子烟使用者的金属暴露和早期心血管风险

• 批准号: 10650778
• 负责人: Ana Navas-Acien
• 金额: $ 64.05万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-01 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10650778
• 关键词: Accounting; Adult; Aerosols; Age Years; Antimony; Arsenic; Atherosclerosis; Biological; Biological Availability; Biological Markers; Blood; Blood Pressure; Blood Vessels; Blood specimen; C-reactive protein; Cadmium; Calcium; Cardiac; Cardiotoxicity; Cardiovascular Diseases; Cardiovascular system; Characteristics; Chromium; Clinical; Clinical Research; Consumption; Cross-Over Studies; Data; Devices; Disease Marker; Dose; Electronic cigarette; Endothelial Cells; Endothelium; Engineering; Ethnic Origin; Future; Health; Heart Rate; Heating; Image; Impairment; Inflammation; Interleukin-6; Knowledge; Laboratories; Lead; Long-Term Effects; Longitudinal Studies; Manganese; Measures; Mediating; Mediation; Medicine; Metal exposure; Metals; Modeling; Myocardial Infarction; New York City; Nickel; Nicotine; Nitric Oxide; Outcome Assessment; Oxidative Stress; Participant; Pathway interactions; Pattern; Persons; Questionnaires; Regulation; Risk; Role; Sampling; Scanning; Smoke; Smoker; Smoking; Source; Testing; Tobacco smoke; Toxic effect; Tungsten; United States National Academy of Sciences; Urine; Vasodilation; Visit; Woman; X-Ray Computed Tomography; Zinc; blood lead; cardiovascular disorder risk; cardiovascular effects; cardiovascular health; cardiovascular risk factor; cell injury; clinical practice; coronary artery calcification; e-cigarette aerosols; electronic cigarette use; electronic cigarette user; electronic liquid; epidemiology study; experience; experimental study; former smoker; men; metallicity; multidisciplinary; never smoker; particle; public health intervention; racial diversity; recruit; smartphone based assessment; smoking cessation; tobacco regulatory science; tobacco smokers; tool; toxic metal; toxicant; vaper; vaping; young adult

PROJECT SUMMARY Despite limited knowledge about their toxicity and health effects, the use of electronic cigarettes (e-cigs) has increased among former smokers and young adults who have never smoked. Increasing evidence shows that e-cig aerosol contains relatively high levels of toxic metals, including lead and nickel, metals with strong evidence for a role in cardiovascular (CV) disease. The objective of this proposal is to evaluate (1) the association of e-cig use with metal exposure, (2) the association of e-cig use with coronary artery calcification and other relevant pathways (endothelial cell health, blood pressure and inflammation), and (3) the potential role of e-cig metals to explain, at least in part, the CV effects of e-cigs. In preliminary studies, we found marked increases in metal levels in the generated aerosol compared to e-liquid from the dispenser from devices of daily e-cig users, demonstrating that the heating coil is a source of metal exposure. Metals in e-cig aerosols, moreover, were positively associated with metal biomarker levels. We will conduct a longitudinal study of men and women 18 to 50 years of age of diverse race/ethnic backgrounds from New York City (130 never tobacco smokers or e-cig users (controls), 130 e-cig users who are never tobacco smokers, 130 e-cig users who are former smokers, and 130 e-cig users who are current smokers and evaluate each participant 3 times over a 1.5-year period (month 1, 9, and 18). For exposure assessment, we will collect data on e-cig use and use patterns from a questionnaire and 4-weekly mobile assessments (month 3, 6, 12, 15), an aerosol sample from the participants' e-cig device and urine and blood samples to measure metals. For outcome assessment, we will measure coronary artery calcification (CAC) using cardiac CT-scan at baseline estimating the spatially weighted calcium score (SWCS). The SWCS provides a valid, continuous measure of CAC suitable for quantifying the extent of atherosclerosis without a threshold. At each visit will also measure endothelial cell health through endothelium-dependent arterial vasodilation using EndoPAT2000 device and endothelial cells- derived microparticles (EMPs) (a marker of endothelial cell injury), blood pressure, and biomarkers of inflammation (C-reactive protein and interleukin-6). We will estimate differences in metal biomarkers and in CV risk comparing e-cig users to non-users and by e-cigarette use patterns (e.g., type of device, number of puffs, e-liquid consumed per week, type of coil). In a formal mediation analysis, we will assess the association of e- cig use with the CV measures accounting for the mediating role of metals. The proposed longitudinal study utilizes interdisciplinary expertise in the characterization of CV disease, assessment of metals, the recruitment and examination of e-cig users, and the general field of tobacco regulatory science, to fill a critical gap in the knowledge about the possible long-term effects of e-cig use and to help inform e-cig regulatory action.

项目概要 尽管对其毒性和健康影响的了解有限，但电子烟的使用已 在前吸烟者和从未吸烟的年轻人中有所增加。越来越多的证据表明 电子烟气溶胶含有相对较高含量的有毒金属，包括铅和镍，这些金属具有强 在心血管（CV）疾病中发挥作用的证据。本提案的目的是评估 (1) 电子烟使用与金属暴露的关联，(2) 电子烟使用与冠状动脉钙化的关联 和其他相关途径（内皮细胞健康、血压和炎症），以及（3）潜力 电子烟金属的作用至少部分解释了电子烟的CV效应。在初步研究中，我们发现显着 与来自设备的分配器的电子液体相比，产生的气溶胶中的金属含量增加 日常电子烟用户，证明加热线圈是金属暴露的来源。电子烟气溶胶中的金属， 此外，与金属生物标志物水平呈正相关。我们将对男性进行纵向研究 以及来自纽约市的 18 至 50 岁不同种族/民族背景的女性（130 名从不吸烟 吸烟者或电子烟用户（对照）、130 名从不吸烟的电子烟用户、130 名吸烟者 前吸烟者和 130 名当前吸烟者的电子烟用户，并对每个参与者进行 3 次评估 1.5 年期间（第 1、9 和 18 个月）。为了进行暴露评估，我们将收集电子烟使用和使用的数据 调查问卷和每 4 周移动评估（第 3、6、12、15 个月）的模式，气溶胶样本来自 参与者的电子烟设备以及尿液和血液样本来测量金属。对于结果评估，我们 将在基线时使用心脏 CT 扫描测量冠状动脉钙化 (CAC)，估计空间 加权钙评分（SWCS）。 SWCS 提供了有效、连续的 CAC 测量，适用于 无阈值地量化动脉粥样硬化的程度。每次访视时还将测量内皮细胞 使用 EndoPAT2000 装置和内皮细胞通过内皮依赖性动脉血管舒张来促进健康 - 衍生微粒 (EMP)（内皮细胞损伤的标志物）、血压和生物标志物 炎症（C 反应蛋白和白细胞介素 6）。我们将估计金属生物标志物和 CV 的差异 通过电子烟使用模式（例如设备类型、抽吸次数、 每周消耗的电子液体，线圈类型）。在正式的中介分析中，我们将评估电子的关联性 cig 的使用与 CV 措施一起解释了金属的中介作用。拟议的纵向研究 利用跨学科专业知识来表征心血管疾病、金属评估、招募 对电子烟用户以及烟草监管科学的一般领域进行检查，以填补这一领域的关键空白 了解电子烟使用可能产生的长期影响，并帮助为电子烟监管行动提供信息。

项目成果

Semi-Automatic Graphical Tool for Measuring Coronary Artery Spatially Weighted Calcium Score from Gated Cardiac Computed Tomography Images.

用于从门控心脏计算机断层扫描图像测量冠状动脉空间加权钙分数的半自动图形工具。

• DOI: 10.3791/65458
• 发表时间: 2023
• 期刊: Journal of visualized experiments : JoVE
• 作者: Patel,HeliJ;Kaufman,AudreyE;Pereañez,Marco;Soultanidis,Georgios;Ramachandran,Sarayu;Naidu,Sonum;Mani,Venkatesh;Fayad,ZahiA;Robson,PhilipM
• 通讯作者: Robson,PhilipM