Molecular Interactions and Imaging Core

分子相互作用和成像核心

基本信息

批准号：
10647704
负责人：
HENRY N HIGGS
金额：
$ 44.63万
依托单位：
DARTMOUTH COLLEGE
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-05-15 至 2026-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10647704
关键词：
Actins Adopted Area Award Awareness Binding Biochemical Biological Assay Biology Calorimetry Cells Centers of Research Excellence Collaborations Collection Communities Consultations Core Facility Cryoelectron Microscopy DNA Repair Data Data Analyses Development Drosophila genus Electron Microscopy Electron Microscopy Facility Embryo Endocytosis Ensure Environment Equipment Experimental Designs Extramural Activities Faculty Fluorescence Microscopy Funding Goals Grant Histone H3 Human Image Image Analysis Imaging technology In Situ In Vitro Individual Influenza Hemagglutinin Infrastructure Institution Laboratories Lead Location Mammalian Cell Mass Spectrum Analysis Measures Melanoma Cell Methods Microscopy Mission Modeling Molecular Molecular Structure Monitor Monoclonal Antibodies Norris Cotton Cancer Center Nuclear Organelles Participant Phase Pilot Projects Play Polymers Population Positioning Attribute Process Production Program Reviews Protein Dynamics Protein Isoforms Proteins Proteomics Recovery Research Research Infrastructure Research Personnel Research Project Grants Resolution Resources Role Scientist Services Specialist Spectrum Analysis Strategic Planning Structure Surface Plasmon Resonance Surveys T-Lymphocyte Talents Techniques Technology Time Titrations Training Validation Virus Visualization Water Work Workload biophysical properties biophysical techniques cell motility cellular imaging cost cost effective design experimental study flexibility fluorescence microscope imaging study improved instrument instrumentation interest intravital imaging light microscopy member method development molecular imaging operation polymerization pressure protein purification recruit success superresolution microscopy tool tumor

Actins Adopted Area Award Awareness Binding Biochemical Biological Assay Biology Calorimetry Cells Centers of Research Excellence Collaborations Collection Communities Consultations Core Facility Cryoelectron Microscopy DNA Repair Data Data Analyses Development Drosophila genus Electron Microscopy Electron Microscopy Facility Embryo Endocytosis Ensure Environment Equipment Experimental Designs Extramural Activities Faculty Fluorescence Microscopy Funding Goals Grant Histone H3 Human Image Image Analysis Imaging technology In Situ In Vitro Individual Influenza Hemagglutinin Infrastructure Institution Laboratories Lead Location Mammalian Cell Mass Spectrum Analysis Measures Melanoma Cell Methods Microscopy Mission Modeling Molecular Molecular Structure Monitor Monoclonal Antibodies Norris Cotton Cancer Center Nuclear Organelles Participant Phase Pilot Projects Play Polymers Population Positioning Attribute Process Production Program Reviews Protein Dynamics Protein Isoforms Proteins Proteomics Recovery Research Research Infrastructure Research Personnel Research Project Grants Resolution Resources Role Scientist Services Specialist Spectrum Analysis Strategic Planning Structure Surface Plasmon Resonance Surveys T-Lymphocyte Talents Techniques Technology Time Titrations Training Validation Virus Visualization Water Work Workload biophysical properties biophysical techniques cell motility cellular imaging cost cost effective design experimental study flexibility fluorescence microscope imaging study improved instrument instrumentation interest intravital imaging light microscopy member method development molecular imaging operation polymerization pressure protein purification recruit success superresolution microscopy tool tumor

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项目摘要

The Molecular Interactions and Imaging Core (MIIC) increases the rigor and sophistication of bioMT research projects by facilitating the use of molecular binding assays and imaging technologies. One goal of the MIIC is to eliminate barriers to these technologies and provide advice in the optimal collection and analysis of the data. In phase I of this grant, MIIC has centralized oversight of existing resources and participated in the acquisition of new equipment, both within the bioMT facility and in conjunction with two existing microscopy cores at Dartmouth. We have hired staff to provide training, guidance, and experimental support in the characterization of molecular interactions. We have also hired two imaging navigators to help investigators with experimental design, image acquisition, and image analysis. These activities significantly enhanced the ability of our phase I research project leaders (RPLs) and all bioMT investigators to conduct quantitative binding and imaging studies at Dartmouth, and thus contributed to their extramural and academic success. Our plans for the next funding period have been guided by an inclusive and intensive program review process. In phase II, MIIC has four specific aims. Specific Aim 1 is to provide customized support to help the four RPLs attain their research goals and achieve independence. As active participants in the development of the four research projects, the director and research navigators engaged with the RPLs to comprehensively assess the methods required by each. From this survey we identified key technologies to quantify binding interactions, to visualize molecular locations and dynamics of proteins and organelles in cells, and to assess distribution of cells within tumors in situ. Specific Aim 2 is to expand our services to three key areas: mass spectrometry/proteomics, higher resolution cellular imaging by super-resolution microscopy and electron microscopy, and cryo-electron microscopy analysis of macromolecular structure. In all three areas, MIIC will continue the cost-effective practice of leveraging existing equipment at Dartmouth and elsewhere to accomplish these goals. Specific Aim 3 is to extend support for image analysis, which is an increasingly pressing need for many investigators. Specific Aim 4 prepares for sustainability of the Core in phase 3. The MIIC works closely with the Molecular Tools Core, which is housed in the same, newly renovated bioMT Core Facilities Suite. In addition, the MIIC has established strong working relationships with existing cores at Dartmouth (including several supported by other COBRE awards) as well as at other IDeA partner institutions. Within all four aims, the MIIC has been designed to adapt flexibly to technological needs that emerge as bioMT investigators make scientific progress. The MIIC will also share best practices and ensure up-to-date training for all bioMT investigators interested in monitoring, and in most cases quantifying, binding interactions of their biomolecules, whether as purified components or within cells. It will thus directly support all four bioMT Research Projects as well as affiliated bioMT faculty, and will enhance the research infrastructure available at Dartmouth and among our regional IDeA partners.

分子相互作用和成像核（MIIC）增加了生物T研究的严格和复杂性通过促进分子结合测定和成像技术的使用项目。 MIIC的一个目标是消除这些技术的障碍，并在数据的最佳收集和分析中提供建议。在这笔赠款的第一阶段，MIIC集中了对现有资源的监督，并参与了收购新设备，包括在生物T设施内以及与两个现有显微镜核的结合达特茅斯。我们雇用了员工来提供培训，指导和实验支持。分子相互作用。我们还雇用了两个成像导航器来帮助调查人员进行实验设计，图像采集和图像分析。这些活动大大提高了我们第一阶段的能力研究项目负责人（RPLS）和所有BIOMT研究人员进行定量结合和成像研究在达特茅斯（Dartmouth），因此为他们的壁外和学术成就做出了贡献。我们的下一个资金计划期间以包容性和密集的计划审查过程为指导。在第二阶段，MIIC有四个具体目标。具体目标1是提供定制的支持，以帮助四个RPL实现其研究目标并实现独立。作为四个研究项目开发的积极参与者，董事和研究导航器与RPL互动，以全面评估每种方法所需的方法。从这项调查中，我们确定了关键技术以量化结合相互作用，以可视化分子位置细胞中蛋白质和细胞器的动力学，并评估原位肿瘤内细胞的分布。具体的 AIM 2是将我们的服务扩展到三个关键领域：质谱/蛋白质组学，较高的分辨率蜂窝通过超分辨率显微镜和电子显微镜进行成像，以及冷冻电子显微镜分析大分子结构。在这三个领域，MIIC将继续利用现有的经济高效的做法达特茅斯和其他地方的设备来实现这些目标。特定目标3是扩展对图像的支持分析，这是许多研究人员越来越紧迫的需求。特定目标4为可持续发展做准备第3阶段中的核心。MIIC与分子工具核心密切合作，该工具核心构成相同的分子工具核心新装修的Biomt核心设施套件。此外，MIIC建立了牢固的工作关系在达特茅斯的现有核心（包括其他山毛奖的支持）以及其他想法合作伙伴机构。在所有四个目标中，MIIC旨在灵活地适应技术需求随着BIOMT研究人员取得了科学进步，这一兴起。 MIIC还将分享最佳实践，并确保对所有对监视感兴趣的生物群的研究人员的最新培训，并且在大多数情况下进行量化，约束力它们的生物分子的相互作用，无论是纯化的成分还是在细胞内。因此，它将直接支持所有四个BIOMT研究项目以及附属的BIOMT教师，并将增强研究基础设施可在达特茅斯（Dartmouth）以及我们的区域思想合作伙伴中找到。